Adhere cells onto slides for immunostaining

Susan Hubchak hubchak at nwu.edu
Fri Feb 28 03:24:18 EST 1997


Assuming you mean cultured mammalian cells, try coating glass with 1 mg/ml
tissue culture grade gelatin (dissolved in high quality water) at 37C for
30-60 min. before plating.  Just remove the gelatin solution before adding the
cells, no rinsing needed.

Susan Hubchak
hubchak at nwu.edu




In article <5f68cg$s0a at nuscc.nus.sg>, imazhq at leonis.nus.sg (Zheng
Huanquan) wrote:

> Hi, everyone, Would you please provide me with the best method for cell 
> adhere onto slide for immunofluorecence staining? I tried to polysine 
> coated slides, alginic acid, but still lose a lot of cells after staining.
> 
> Hugo ZHENG



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