Hot plasmid + Cold Vector = Insert-specific probe????
kraev at bc.biol.ethz.ch
Fri Feb 28 06:59:41 EST 1997
In article <prigge-ya023680002702971546450001 at pith.uoregon.edu>,
prigge at darkwing.uoregon.edu (Michael J. Prigge) wrote:
> I need to screen a cDNA library with a BAC with a ~100 kb insert.
> I thought I read somewhere that one could label the whole plasmid,
> then add lots of cold vector to the hyb solution. This might
> theoretically result in no hybridization to the vector sequences
> of the plaques.
A good procedure that can be easily adapted for your needs is described in
Nucleic Acids Res., vol. 23(6), 1085 (1995). I used it once with
don't have much statistics ( who has it, other than those in a genome centre).
I hope this helps,
Alexander Kraev, PhD
Biochemie III, ETHZ Zurich
e-mail kraev at bc.biol.ethz.ch
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