Hot plasmid + Cold Vector = Insert-specific probe????

Alexander Kraev kraev at
Fri Feb 28 06:59:41 EST 1997

In article <prigge-ya023680002702971546450001 at>,
prigge at (Michael J. Prigge) wrote:

> I need to screen a cDNA library with a BAC with a ~100 kb insert. 
> I thought I read somewhere that one could label the whole plasmid, 
> then add lots of cold vector to the hyb solution. This might 
> theoretically result in no hybridization to the vector sequences 
> of the plaques. 

A good procedure that can be easily adapted for your needs is described in
Nucleic Acids Res.,  vol. 23(6),  1085  (1995).  I used it once with
success, but
don't have much statistics ( who has it, other than those in a genome centre).

I hope this helps,

Alexander Kraev, PhD
Biochemie III, ETHZ Zurich
Phone 41-1-632-31-47
Fax 41-1-632-12-13
e-mail kraev at

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