A little help here...
The Kindly One
smccull at jurassic.fisher.calpoly.edu
Fri Feb 28 22:10:36 EST 1997
Maybe someone can help me here. I have made Minimum Essential Medium
for a fibroblast cell line and the papers from NIGMS call for
2x essent and non-essen amino acids. If I make the media at
pH 7.2 so that it's ~7.4 after filtering, then addition of
the AA drop sthe medi to a bright yellow color that I'm sure is
too acidic to be healthy. Any ideas(besides adding AA before filtering)
** Scott D. McCulloch(Bunny Lover) | If we knew what we were doing,
** smccull at jurassic.fisher.calpoly.edu | it would not be called
** BioChemical Effects Research | research, now would it?
** http://www.calpoly.edu/~smccullo/ |
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