differentiating between genomic & plasmid DNA

J. Pat Martinez martinez at puccini.CRL.umn.edu
Fri Feb 28 11:44:59 EST 1997


One way to eliminate non-supercoiled DNA is to use Dnase Dependent DNAse
from epicentre.  This enzyme will only degrade linear DNA, leaving the
supercoiled DNA intact.

Pat M.


> E.Hillery (e.hillery at ic.ac.uk) wrote:
> : Would anybody out there have any thoughts on how I could differentiate
> : between eukaryotic genomic and pg amounts of plasmid DNA.  In an ideal
> : world, I wouls like to separate the two species so that I could then
> : quantify the latter
> 
> : many thanks
> : Liz Hillery
> : Ion Transport                                  'phone: 171 352 8121 x3393
> : National Heart & Lung Institute                 fax: 171 351 8340
> : Manresa Road                                    e-mail:E.Hillery at ic.ac.uk
> : London
> : SW3 6LR



More information about the Methods mailing list