differentiating between genomic & plasmid DNA

[J. Pat Martinez]

One way to eliminate non-supercoiled DNA is to use Dnase Dependent DNAse
from epicentre.  This enzyme will only degrade linear DNA, leaving the
supercoiled DNA intact.

Pat M.


> E.Hillery (e.hillery at ic.ac.uk) wrote:
> : Would anybody out there have any thoughts on how I could differentiate
> : between eukaryotic genomic and pg amounts of plasmid DNA.  In an ideal
> : world, I wouls like to separate the two species so that I could then
> : quantify the latter
> 
> : many thanks
> : Liz Hillery
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