Methods February 1997 Archives by author

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Starting: Sat Feb 1 00:52:13 EST 1997
Ending: Fri Feb 28 22:10:36 EST 1997
Messages: 1105

• oligo biotinylation?   Yi Zhang (BIO)
• Need tips for sequencing through poly-c tail  
• screening of cDNA library: custom services   --
• Linscott's Directory   .
• Which Email reader?   Labor 1
• rRNA contamination after RNA poli-A purification with oligo-dT   CARLOS ALEXANDRE SANCHEZ FERREIRA - 218
• Sequencing and sharkteeth   Edwin Abeln
• Silver Staining Reveals Contaminating Bands in Sample Buffer   Achim Recktenwald, PhD
• Q:Measurement of protein's surface hydrophobicity?   Achim Recktenwald, PhD
• pH of water; dissolving RNA   Achim Recktenwald, PhD
• Sigma Monoclonal Anti-polyHis   David Ackerley
• LPS-free DNA   Thomas Adam
• Operon primer sequences   Dr Alok Adholaya
• BIOSCI/bionet miniFAQ & Fundraiser   BIOSCI Administrator
• digesting the ends of PCR products   Ashok Aiyar
• DNA sequence comparision   Ashok Aiyar
• How to perform a DNA Methyltransferase assay?   Francisco J. Alarcon
• DD-RTPCR   Lou Alarcon
• Protein Labeling? Casein Kinase?   Thomas J. Albert
• Protein Labeling? Casein Kinase?   Thomas J. Albert
• T4 RNA Ligase   Thomas J. Albert
• DNA analysis software   Betsy L. Alberty
• DNA recovery by phenol/chloroform extraction or GeneClean   Betsy L. Alberty
• biotinylation of BBMV   Edwin Alcantara
• RNA standards for quantitative PCR   Adrian Allan
• Agrobacterium   M Allen
• Agrobacterium   M Allen
• address   Jenny Allsop
• Where to be published   Michael Almeida
• P.E. 9600 thermocyclers-the cooling fix...   Christopher G. Alpha
• GFP and FITC   Danny Altschuler
• retroviral particle size?   Danny Altschuler
• double labeling with BrDU   Danny Altschuler
• epitope tagging   Thomas R. Anderson
• monoclonal antibody for HA   Thomas R. Anderson
• 3-Amino triazole conc.?   Andy
• Dexamethasone-inducible pMAMneo vector   Marit Anthonsen
• Q:Measurement of protein's surface hydrophobicity?   Bill Van Antwerp
• Check   Shiwani Arora
• Q: inducible promoters for plants   Curt Ashendel
• How much thrombin for GST fusions ?   Curt Ashendel
• pH of water; dissolving RNA   Curt Ashendel
• Making Nickel resin??   Curt Ashendel
• Which Email reader?   Graham Atherton
• Which Email reader? - Results   Graham Atherton
• Limited digestion   Daniel Auerbach
• SDF-1 antibodies to get or buy   Morgane BOMSEL
• SDF-1 antibodies   Morgane BOMSEL
• Help: Bacterial Genomic DNA extraction and library construction   Joel Bader
• nylon mesh for hybridizations   Marilyn Bakker
• "Oligonucleotide purification method??"   Balwi
• Checking labelling oligo   Balwi
• Subtracted cDNA Probe   Barbara
• LA PCR smuck   Wayne M. Barnes
• DNA extract with CTAB   Tim Barnett
• Stable Thp1 transfections   Guriq Basi
• Article Search   Martin Beaulieu
• Sea Urchin and Manduca cDNA libraries   Greg Beck
• Single Copy Plasmids   Brett Beitzel
• e. coli expressing hsp90   David R. Bell
• E.Coli Plasmid<--->Chromosome Insertion/Recovery?   Michael Benedik
• Cutting DNA from non-E.coli   Michael Benedik
• PCR and poly A sequences,   Frank Beraud
• Smearing of PCR product   Frank Beraud
• Dissolve my pellet, please!   Josh Berke
• Boehringer DIG experiences   Andrew Bettany
• contamination of glycine by other amino acids ???   Queen of Molecular Biology
• Gridded Membranes   Rory O'Brien. Biotechnology.
• Are there single letter codes for phosphoamino acids   Shaun D. Black
• Methicillin suppliers?   Derron Blakely
• E.Coli Plasmid<--->Chromosome Insertion/Recovery?   Mark Blight
• Q: How doing partial RE digests?   Mark Blight
• Collaboration offered: non-LTR retrotransposones study   Alexander Blinov
• HOW to Eliminate PCR primer dimer   Natalie Bloch
• Quntifying small amounts of DNA reliably   Natalie Bloch
• Primer Design   Natalie Bloch
• Quick transformation protocol wanted   A.P.N. de Boer
• Quick transformation protocol wanted   A.P.N. de Boer
• Limited digestion   Thon de Boer
• HPRT targeting vector   Constanze Bonifer
• FLAG epitope in yeast   Jose Bonner
• A killer is needed   Alex Borovkov
• (no subject)   Joan Boyce
• Monoclonal CAT   Joan Boyce
• E.Coli Plasmid<--->Chromosome Insertion/Recovery?   Chris Boyd
• XGAL & IPTG -- how low can you go?   Chris Boyd
• DNA recovery by phenol/chloroform extraction or GeneClean   Chris Boyd
• dam-/dcm- bugs and mutations??   Chris Boyd
• Looking for ColV plasmid   Chris Boyd
• test   Ray Boykiw
• test   Ray Boykiw
• Transposon mutagenesis   Jeffrey David Bray
• Quntifying small amounts of DNA reliably   Gerome Breen
• Membrane preps   Brenda
• Q:measurement of protein's surface hydrophobicity   Kurt L Brillhart
• Quick transformation protocol wanted   Charles Brockhouse
• microsatellite loci amplification   Charles Brockhouse
• Q: inducible promoters for plants   Paul A Bucciaglia
• Silver Staining Reveals Contaminating Bands in Sample Buffer   Tom Buckley
• Operon primer sequences   Anita Burger
• PCR/cloning of bacterial communities   Bill Burnett
• How can I assay translational rate ??   Dr E. Buxbaum
• DNA SOFTWARE INFORMATION PLEASE   CLARKEY
• Probing of an expression library with oligopeptides.   CLARKEY
• Non-specific binding of avidin woes.   CLARKEY
• Request for integrin plasmids or antibodies. PLEASE.   CLARKEY
• digesting the ends of PCR products   AMICVS CONSTANTINI
• protein solubility problems   Malcolm M. Campbell
• Protein Extraction Buffers...What's a good homogenization buffer for westerns?   Malcolm M. Campbell
• Stripping Northerns   Martin Cann
• Problems with One Shot INValphaF' from Invitrogen   Martin Cann
• 2mL Concave Gradients???   Lou Cantolupo
• Transfection   Lou Cantolupo
• HOW to Eliminate PCR primer dimer   Hedley Carr
• Need gp160 clones for HIV IIIB, MN, BRU   George M. Carter
• PCR Techniques Workshop   Mr. Ken Carter
• rDNA Techniques Workshop   Mr. Ken Carter
• ADVANE IMMUNOFLUORESCENE COURSE   Paolo Castano
• PCR primer-dimer elimination   Robert B. Chadwick
• Boehringer DIG system experiences   Mike Challen
• Pepsin: IU to FIP unit ????   H Chang
• Error rate in Taq pol. 1:270?   Tom Chappell
• Error rate in Taq pol. 1:270?   Tom Chappell
• Pfu polymerase in PCR   Tom Chappell
• Adhesive temperature indicator / warning for reagants & buffers   Charles_Lasitter
• Reamplification pcr products after Ag-staining   C.K. Chen
• how to identify a gene on human chromsome 11   K-John Cheung
• no-BglI plasmid   Peter Cheung
• Short seq search?   Chihara
• degenerate PCR   Tim Chipman
• Dissolve my pellet, please!   Christoph.Lorra at urz.uni-heidelberg.de
• pH for RNA work   Shi-Jye Chu
• Restriction Enzymes in DNA Strider?   Namjin Chung
• Mechanism of EMS Action?   Namjin Chung
• Inexpensive Oligo Purification?   Namjin Chung
• Emission wavelength of EtBr/DNA for a CCD camera filter   Dr. Duncan Clark
• Emission wavelength of EtBr/DNA for a CCD camera filter   Dr. Duncan Clark
• High G/C PCR   Dr. Duncan Clark
• Q: purifying lambda phage from extraneous DNA   Dr. Duncan Clark
• Q: purifying lambda phage from extraneous DNA   Dr. Duncan Clark
• Pfu polymerase in PCR   Dr. Duncan Clark
• Error rate in Taq pol. 1:270?   Dr. Duncan Clark
• Pfu polymerase in PCR   Dr. Duncan Clark
• Error rate in Taq pol. 1:270?   Dr. Duncan Clark
• Error rate in Taq pol. 1:270?   Dr. Duncan Clark
• Error rate in Taq pol. 1:270?   Dr. Duncan Clark
• Error rate in Taq pol. 1:270?   Dr. Duncan Clark
• Pfu polymerase in PCR   Dr. Duncan Clark
• Pfu polymerase in PCR   Dr. Duncan Clark
• E. coli (Bl21DE3) lyses automatically   Dr. Duncan Clark
• Pfu polymerase in PCR   Dr. Duncan Clark
• dam-/dcm- bugs and mutations??   Dr. Duncan Clark
• Stop codon read-through?   Dr. Duncan Clark
• removal of PEG from DNA disolved in TE?   Dr. Duncan Clark
• Pfu polymerase in PCR   Dr. Duncan Clark
• dam-/dcm- bugs and mutations??   Dr. Duncan Clark
• dam-/dcm- bugs and mutations??   Dr. Duncan Clark
• Molecular modelling capabilities   Dr. Duncan Clark
• Pfu polymerase in PCR   Dr. Duncan Clark
• long PCR with dUTP   Dr. Duncan Clark
• Real time PCR measurement   Dr. J.P. Clewley
• PCR contamination   Dr. J.P. Clewley
• Plant m-gfp5-ER (Haseloff)   Mark Cock
• DNA recovery by phenol/chloroform extraction or GeneClean   William Coetzee
• multiplying bands in gel purification   William Coetzee
• Transfection   Steve Cohen
• 220 RNA peak   Shelley Cole
• ?-easiest clean-up for sequencing   Dr S.K. Coleman
• pH of water; dissolving RNA   Hildur V. Colot
• GFP S65T vectors   Hildur V. Colot
• Oligo problems   The Great American Gene Company
• Problems with synthetic gene synthesis   The Great American Gene Company
• Inosine in degenerate primers   The Great American Gene Company
• Oligos: Who is cheapest?   The Great American Gene Company
• Inexpensive Oligo Purification?   The Great American Gene Company
• tempest on a toothpick   Tom Cooper
• nylon mesh for hybridizations   Marc Cortese
• Which Email reader?   Joao V. Costa
• Custom DNA-Sequencing in Europe   Francois Coulier
• E. coli (Bl21DE3) lyses automatically   Albert Crescenzo
• DNA Sequencing   DSF
• Chromosomal DNA carry-over in plasmid mini-preps   DSF
• Co-immunoprecipitation of nuclear proteins   Richard Dahl
• test   Richard Daines
• Phenol Protocol   David L. Haviland, Ph.D.
• I need more infor about versene   David L. Haviland, Ph.D.
• Calculation of transformation efficiency   David L. Haviland, Ph.D.
• subcloning large fragments   David L. Haviland, Ph.D.
• pH for RNA work   David L. Haviland, Ph.D.
• [Q]What is PMA converted to ?   David L. Haviland, Ph.D.
• XGAL & IPTG -- how low can you go?   David L. Haviland, Ph.D.
• XGAL & IPTG -- how low can you go?   David L. Haviland, Ph.D.
• Lamda phage - RIP?   David L. Haviland, Ph.D.
• ?-easiest clean-up for sequencing   David L. Haviland, Ph.D.
• Hydropathy plot software wanted   Oliver S. DePeyer
• Biorad Quantum minipreps re ALF sequencers   Oliver S. DePeyer
• Ph.D. Assistantship Available   Dr. Jeffrey Dean
• Wheat germ agglutinin staining.   A.D. Delduca
• adult-specific transgene promoter; dimattia@julian.uwo.ca   Gabriel E. DiMattia
• Problem with nonspecific binding   DiverNLB
• Q: purifying lambda phage from extraneous DNA   John Dixon
• beads for cell culture   Dlh4image
• A solution for DNA or RNA contamination   Dnacleaner
• The solution for contamination   Dnacleaner
• Problems of contaminations? Think DNACleaner...   Dnacleaner
• PCR, NASBA, bDNA. Contaminations disturb your works?   Dnacleaner
• Simple, economic, DNAcleaner: a solution for contaminations   Dnacleaner
• You use frequently PCR or other technics...This is for you!!!   Dnacleaner
• DNAcleaner, french laboratories already use it against contaminations.   Dnacleaner
• RNA from tiny amounts of tissue?   Alex Dobrovic
• availability of cell lines with t(11;14) translocation   Alex Dobrovic
• pH of water; dissolving RNA   Alex Dobrovic
• Westerns and estrogen receptors   Aideen O Doherty
• GFP and FITC   Andrew Doherty
• paraformalduhyde fixation and membrane integrity   Andrew Doherty
• Calcium Phosphate Precipitation   Andrew Doherty
• Pfu polymerase in PCR   Colin Dolphin
• ZAP systems   F Dong
• Hirt extractions   Graeme J. Dougherty
• Making Nickel resin??   Stephen W Doughty
• Taq polymerase inhibition reference   Michael Douglas
• ?-easiest clean-up for sequencing   Tom Duda
• paranitrophenyl acetate   Bernard Dumas
• Pfu polymerase in PCR   Tom Duncan
• Pfu polymerase in PCR   Tom Duncan
• Pfu polymerase in PCR   Tom Duncan
• "Oligonucleotide purification method??"   Paul Dunman
• DNA recovery by phenol/chloroform extraction or GeneClean   Paul Dunman
• Hybrizab libraries   Daniel Durocher
• How much DNA can be "crowded" into a solution?   E.Hillery
• differentiating between genomic & plasmid DNA   E.Hillery
• transfection/transformation   E.Hillery
• nylon mesh for hybridizations   EHeilweil
• Mark.Ebdon@lifesciences.com   Mark Ebdon
• Rodent (mouse or rat) microsat. primers wanted....   Dave Edwards
• Disposable counting chamber   Eike
• Q: inducible promoters for plants   Dr. K. Eimert
• phosphatase detection in yeast ...   Daniel O. Espinoza
• error rate reverse transcriptases   F.Schaap at FYS.UNIMAAS.NL
• HOW to Eliminate PCR primer dimer   Frank O. Fackelmayer
• Fragility of plasmids: size limit for shaking/vortexing?   Frank O. Fackelmayer
• Error rate in Taq pol. 1:270?   Frank O. Fackelmayer
• DNA recovery by phenol/chloroform extraction or GeneClean   Frank O. Fackelmayer
• Pfu polymerase in PCR   Frank O. Fackelmayer
• Are these primer dimers?   Frank O. Fackelmayer
• Using a PC as an assay counter-timer...   Frank O. Fackelmayer
• cDNA Library   Patrick HJ Falckh
• hyperfilm-MP/hyperfilm-ECL   Federici
• hyperfilm   Federici
• monoclonal antibody for HA   Krista Fehr
• Monoclonal vs polyclonal antibody   Levon Fendekian
• Boehringer DIG system experiences   Karen Ferri
• Deep Vent Pol (ssDNA)   Fiberhead
• Neomycin/Kanamycin Resistance   C. J. Fields
• Stop codon read-through?   C. J. Fields
• Stop codon read-through?   C. J. Fields
• mean stacking temp. algorithm   Michael Finney
• Vol of ligation mix to use in transformation   Karl Fischer
• Q: purifying lambda phage from extraneous DNA   Karl Fischer
• sticky surfaces (fwd)   Karl Fischer
• translational control ??   Karl Fischer
• quick question   Karl Fischer
• quick question   Karl Fischer
• Single Copy Plasmids   Karl Fischer
• what kind of film?   Michael Fitch
• Breakthroughs in SPM   Mark Flowers
• trypsin magnetic beads   Mick Foley
• Error rate in Taq pol. 1:270?   Bryan L. Ford
• I need more infor about versene   Forget-me-not
• Q: How doing partial RE digests?   Lee Fradkin
• Yeast Multicopy Library Needed   Toby Freedman
• Are there single letter codes for phosphoamino acids   Peter Friedhoff
• Hirt procedure pl.   Patil J G
• Mac-based commercial mapping program   GEN282E at CCS1.cc.monash.edu.au
• integrin antibody   ANNE GEORGE
• Screening for Amp(s) bacteria.   Soeren G. Gatermann
• SR-B1 (scanvenger receptor B1) required   Benoit Gauthier
• (none)   Benoit Gauthier
• RNA mobility shift assay.   Morten Andreas Geday
• Na-acetate in RNA   Dr. Peter Gegenheimer
• Pfu polymerase in PCR   Dr. Peter Gegenheimer
• Q: How to remove small size RNA from total RNA in bacteria?   Dr. Peter Gegenheimer
• quick question   Rani Geha
• Stability of Doxycycline?   Stephan Geley
• Transient transfection of Cos-7 (electroporation v DEAE dextran)   George
• Screening for Amp(s) bacteria.   Szatmari George
• refolding from inclusion bodies   Sourav Ghosh
• Disposal of Hoechst waste   J. G. Gibbings
• YAC libraries   J. G. Gibbings
• superantigen genes.help.alternative method to Southern hybridisation sought   Frans and Magda van Gils
• superantigen genes.help.alternative method to Southern hybridisation sought   Frans and Magda van Gils
• superantigen genes.help.alternative method to Southern hybridisation sought   Frans and Magda van Gils
• IMPORTANT: Eukaryotic vector sequences!   Ole Gjoerup
• DNA-free RNA   Michelle Gleeson
• nylon mesh for hybridizations   Michelle Gleeson
• electrophoresis units recommendations?   Michelle Gleeson
• Sequencing and sharkteeth   Michelle Gleeson
• blotting acrylamide gels   Michelle Gleeson
• Invitation to BioMOO   Gustavo Glusman
• digesting the ends of PCR products   Steven Goldberg
• Imidazole buffer has esterase activity?   Steven Goldberg
• Looking for ColV plasmid   Steven Goldberg
• DNA recovery by phenol/chloroform extraction or GeneClean   Steven Goldberg
• Questions about DNA recovery and cloning   Steven Goldberg
• Question about genomic library construction   Steven Goldberg
• Molecular biology software suggestions   Josh Goldstein
• High G/C PCR   Josh Goldstein
• RNA Splicing Software   Jose Gonzales
• Green Fluorescent Protein Purificaton Short Course   Daniel Gonzalez
• Bordetella FHA   James Good
• Bordetella FHA   James Good
• Bordetella FHA   James Good
• bright specks in EtBr stained gels - out, damn'd spot!   Martin Goodson
• Conductance Standards   Frank R. Gorga
• long PCR with dUTP   Michael C. Gorry
• yeast two hybrid   Martin Gossen
• 3-Amino triazole conc.?   Martin Gossen
• Acetamide in PCR reaction?   Anton Scott Goustin
• transfection/transformation   Anton Scott Goustin
• How can I assay translational rate ??   Anton Scott Goustin
• Jurkatt cell induction   Anton Scott Goustin
• 2D peptide mapping   Roney Graf
• 293 cells in serum-free medium?   Lloyd Graham
• test - please ignore   Granny
• I need more infor about versene   Richard P Grant
• Error rate in Taq pol. 1:270?   Richard P Grant
• paraformalduhyde fixation and membrane integrity   Richard P Grant
• removal of PEG from DNA disolved in TE?   Richard P Grant
• Strange contaminant in cell culture   Richard P Grant
• northern blotting   Sam Gray
• best total RNA and mRNA isolation kits   Sam Gray
• Selling a used Beckmann rotor   Roger C. Green
• Stratagene Site-directed mutagenesis   Greg
• Stratagene QuikChange mutagenesis?   Greg
• Oligos: Who is cheapest?   Greg
• E. coli (Bl21DE3) lyses automatically   Greg
• multiplying bands in gel purification   T.S. Grewal
• DGGE Gel Drying...?   Dr Steve Griffiths Molecular Biology Group
• painful gel loading???!!!!!!   Mike Gruidl
• Two-Hybrid System for E coli ?   Toumy Guettouche
• Streptavidin and Biotin interactions   Toumy Guettouche
• Streptavidin and Biotin interactions   Toumy Guettouche
• Two-Hybrid System for E coli ?   Toumy Guettouche
• **************Gancyclovir**********************   Toumy Guettouche
• DNA recovery by phenol/chloroform extraction or GeneClean   David Hackos
• How much thrombin for GST fusions ?   Tom Haenel
• Transfecting B cells   Erik Kul| Hagen
• PCR-Select cDNA Subtraction Kit   Victor Han
• DD-RTPCR   Victor Han
• IMPACT   Victor Han
• Na+/K+ ATPase assay   J Hance
• Na+/K+ ATPase assay   J Hance
• IL-4 assay on PBMC, help !   Jens Rainer Hansen
• Histone H1 size?   Rizwan Haq
• Primary embryo fibroblasts transfection   Rizwan Haq
• PC restriction mapping software???   Brandon Harder
• yeast 2 hybrid screen on heart cDNA library   Malathi Hari
• heart 2hybrid screen   Malathi Hari
• Clontech GFP   Alex Harvey
• acid phenol extractions   Ben Haskell
• DNA-free RNA   Francois Hatey
• SYPRO Orange Quenching by 35-S   Dick Haugland
• GFP and FITC   Dick Haugland
• imidazole buffer has esterase activity   Dick Haugland
• DNA quantitation   Dick Haugland
• Sequence of pT7-5   Philip Heacock
• Molecular modelling capabilities   George A. Heavner
• PCR detection of RETROVIRUSES   Raymond Henderson
• Pointer to FAQ lists   Paul N Hengen
• Silver Staining Reveals Contaminating Bands in Sample   Paul N Hengen
• Using a PC as an assay counter-timer...   Paul N Hengen
• Error rate in Taq pol. 1:270?   Paul N Hengen
• LA PCR smuck   Paul N Hengen
• Screening for Amp(s) bacteria.   Paul N Hengen
• plasmid DNA needed (50-100 mg)   Paul N Hengen
• Transposon mutagenesis   Paul N Hengen
• Quick transformation protocol wanted   Paul N Hengen
• Screening for Amp(s) bacteria.   Paul N Hengen
• subscribe   Henry A. Daum, III
• subscribe   Henry A. Daum, III
• help-stable horse blood agar-recipes   Michael Heuzenroeder
• Cutting DNA from non-E.coli   Eileen Hickey
• anti-prion protein   Andy Hill
• Gancylovir Supplier   David Hills
• **************Gancyclovir**********************   David Hills
• best total RNA and mRNA isolation kits   Timo Hiltunen
• long PCR with dUTP   Timo Hiltunen
• (none)   Hung-yiu Ho
• (none)   Hung-yiu Ho
• Need tips for sequencing through poly-c tail   Scott Hodgson
• mean stacking temp. algorithm   Les Hoffman
• hydroxymethyl dCTP   Les Hoffman
• Expression of bcl-2 homologue bad   Reinhard Hoffmann
• Which Email reader? - Results   Susan Jane Hogarth
• Using a PC as an assay counter-timer...   Susan Jane Hogarth
• Screening for Amp(s) bacteria.   Susan Jane Hogarth
• Thermal Cyclers, Evaluation   Mark Hogben
• Which Email reader?   Louis Hom
• HELP: where to obtain rat small intestine cDNA library??   Guido Hooiveld
• sequencing signal dies after 100 bases?!   Jan Hoolwerf
• sequencing signal dies after 100 bases?!   Jan Hoolwerf
• Are these primer dimers?   Hopkins, Charlotte
• Are these primer dimers?   Hopkins, Charlotte
• GFP/time-lapse   Neil Hotchin
• gel doc/photo imaging system   Hoyun_Lee at octrf.on.ca
• shrimp allergen   Huangxu at space.ihw.co.cn
• Adhere cells onto slides for immunostaining   Zheng Huanquan
• A freezer inventory software?   Susan Hubchak
• Adhere cells onto slides for immunostaining   Susan Hubchak
• Receptor Ligand Blot   Pierre Hubert
• digesting the ends of PCR products   Martin Hughes
• Pfu polymerase in PCR   Martin Hughes
• GFP expressing cell lines   Jean-Philippe Hugnot
• best total RNA and mRNA isolation kits   Karim Hyder
• best total RNA and mRNA isolation kits   Karim Hyder
• Apparati for Northern Blotting   DiAnna L. Hynds
• Error rate in Taq pol. 1:270?   ICSC
• Cambridge Healthtech Institute’s MOLECULAR EVOLUTION   Cambridge Healthtech Institute
• Seeking secretion vector to express MFalpha-fusion protein   James Irving
• WANTED: Vector to express MF-alpha fusion protein   James Irving
• Growth and transformation of XL1-Blue   Fraser Dr N J
• Pfu polymerase in PCR   Fraser Dr N J
• Inosine in degenerate primers   Fraser Dr N J
• acid enzyme substrate??   ITABAJARA DA SILVA VAZ JUNIOR
• Cloning problem   Nick Jacobsen
• Deep Vent Pol (ssDNA)   Nick Jacobsen
• High G/C PCR   Nick Jacobsen
• pCYPAC2   Nick Jacobsen
• Smearing of PCR product   Nick Jacobsen
• rubidium chloride for making competent cells?   Nick Jacobsen
• Help suppliers of biotinylated magnetic beads   Nick Jacobsen
• hot PCR   Nick Jacobsen
• biometra vs perkin elmer thermal cycler   Di James
• Application notes on respirometery of living organisms   JanCzek
• Application notes on respirometery of living organisms   JanCzek
• Transformation protocol for Bacillus needed   Arul Jayaraman
• Need to find Supplier of Anti-Neo   Blair Jennings
• AFLP plant mapping kit   Jimmy
• Enhancement of HRP substrate systems??   Joep
• Inosine in degenerate primers   Dr. Douglas A. Johnson
• imidazole buffer has esterase activity   Zophonias O. Jonsson
• hot PCR   Rob Jordan
• Northern blot RNA degradation   Jos
• sticky surfaces (fwd)   Pradeep M Joshi
• help with pcr for dna from sediments   Guenter Jost
• E. coli (Bl21DE3) lyses automatically   Justinian
• The solution for contamination   K.James
• Question about genomic library construction   K.James
• SV40 poly(A) signal works bidirectionally?   Alan KAY
• transfection/transformation   Alan KAY
• Crustacean ITS Phylogeny and PCR   Ari Kahn
• T7select phage display   Liselotte Kahns
• Knockout mice advice   Karen
• Knockout mice advice   Karen
• refolding from inclusion bodies   Gouzel Karimova
• Deglycosylation of proteins: questions   Dale Karlson
• HOW to Eliminate PCR primer dimer   Huang Ke-xue
• Km calculation web site   Huang Ke-xue
• Silver Staining Reveals Contaminating Bands in Sample Buffer   Huang Ke-xue
• Stratagene QuikChange mutagenesis?   Huang Ke-xue
• Fusion Protein Expression Systems   Huang Ke-xue
• E. coli (Bl21DE3) lyses automatically   Huang Ke-xue
• Free webzine for BioMedNet members   Huang Ke-xue
• E. coli (Bl21DE3) lyses automatically   Huang Ke-xue
• Question about genomic library construction   Huang Ke-xue
• mAb Purification Difficulties   Patricia Keely
• multiplying bands in gel purification   Jill Kellogg
• Proteinase K and detergent   Jill Kellogg
• Transgenics   Breandan Kennedy
• Quantitative RT-PCR   J Kennie
• Which Email reader?   Gabriele Kerber
• Primer design   Michael Kertesz
• Strange contaminant in cell culture   Obaid Y. Khan
• double labeling with BrDU   Jorg Kirberg
• Image Analysis Shareware   Dima Klenchin
• Transfecting CV1 cells   Dima Klenchin
• retroviral particle size?   Dima Klenchin
• Tris base vs. Trizma   Dima Klenchin
• Qustion on Ammonium sulfate and other precipitation methods   Dima Klenchin
• Transfection of EBV-transformed B cell lines   Dima Klenchin
• Protein Expression   Dima Klenchin
• Strange contaminant in cell culture   Dima Klenchin
• Cosmid cloning recommendations?   Volker Knoop
• INDEX MEDICUS address   Volker Knoop
• Seperation of NAD and nicotinamide by HPLC   Heiko Koch
• Antibody against T7 RNA Polymerase   Alain Kohl
• HDC antibody   Yoshitaka Konda
• 293 cells in serum-free medium?   Jarkko Kortesmaa
• Fragility of plasmids: size limit for shaking/vortexing?   Alexander Kraev
• ssDNA (M13) extract. with silica?   Alexander Kraev
• what kind of film?   Alexander Kraev
• differentiating between genomic & plasmid DNA   Alexander Kraev
• ?-easiest clean-up for sequencing   Alexander Kraev
• Hot plasmid + Cold Vector = Insert-specific probe????   Alexander Kraev
• GST-fusion protein extraction   Cornelius Krasel
• Qustion on Ammonium sulfate and other precipitation methods   Cornelius Krasel
• Which Email reader? - Results   Cornelius Krasel
• what kind of film?   Cornelius Krasel
• "Oligonucleotide purification method??"   Cornelius Krasel
• LB contains glucose?   Cornelius Krasel
• Horseradish Peroxidase assay   Sanjay Krishnaswamy
• Boehringer DIG system experiences   Georg Kroeger
• DNA-based assay of apoptosis needed   Jerry Kropp
• DNA-based assay of apoptosis needed   Jerry Kropp
• Oligo problems   Jerry Kropp
• mean stacking temp. algorithm   Jerry Kropp
• Effect of DMSO on dsDNA?   Jerry Kropp
• High G/C PCR   Jerry Kropp
• Desire recommendation re 2-D gel apparatus   Jerry Kropp
• 2-D software for Mac?   Jerry Kropp
• blotting acrylamide gels   Jerry Kropp
• test...   Gary Kucera
• Kozak concensus sequence   Gary Kucera
• Growth and transformation of XL1-Blue   Alexander N. Kukushkin
• Calculation of transformation efficiency   Alexander N. Kukushkin
• Tris base vs. Trizma   Alexander N. Kukushkin
• electrophoresis at pH 8,0   Alexander N. Kukushkin
• Calcium Phosphate Transient Transfection of NIH/3T3???   Alexander N. Kukushkin
• Cleaning up Transcriptions   Alexander N. Kukushkin
• How many exonucleases have been discovered?   Alexander N. Kukushkin
• Questions about DNA recovery and cloning   Alexander N. Kukushkin
• Question about genomic library construction   Alexander N. Kukushkin
• RNA secondary structure   A. Kumar
• Pins microplates cover   martin LEACH
• painful gel loading???!!!!!!   martin LEACH
• Chromosomal DNA carry-over in plasmid mini-preps   martin LEACH
• plasmid DNA needed (50-100 mg)   martin LEACH
• XGAL & IPTG -- how low can you go?   martin LEACH
• nylon mesh for hybridizations   martin LEACH
• XGAL & IPTG -- how low can you go?   martin LEACH
• Q: How to remove small size RNA from total RNA in Bacteria?   martin LEACH
• Mechanism of EMS Action?   martin LEACH
• Lamda phage - RIP?   martin LEACH
• I need graphics of the number of IgG (y) in function of time (x)   martin LEACH
• Transfection   martin LEACH
• direct genomic sequencing   martin LEACH
• luciferase   martin LEACH
• AATAAA signal   martin LEACH
• Help suppliers of biotinylated magnetic beads   martin LEACH
• DNA sequence comparision   martin LEACH
• nonspecific primer extension products   martin LEACH
• nonspecific primer extension products   martin LEACH
• anti-prion protein   martin LEACH
• Sequencing and sharkteeth   martin LEACH
• quick question   martin LEACH
• quick question   martin LEACH
• Where to be published   martin LEACH
• Looking for cDNA sequence or accession number of IRS-2   martin LEACH
• Gridded Membranes   martin LEACH
• constitutive gene in bacteria   NG WAI LEUNG
• nonspecific primer extension products   Barr Lab
• subcloning large fragments   Frederic G. Barr Lab
• jwc-nondenaturing geo of protein   Loesch-Fries Lab
• Stop codon read-through?   Harris Laboratory
• Transfection of EBV-transformed B cell lines   John Ladasky
• Transfection of EBV-transformed B cell lines   John Ladasky
• DNASpammer   John Ladasky
• Inosine in degenerate primers   John Ladasky
• Pfu polymerase in PCR   John Ladasky
• Long PCR & Joining 5' of sequence to the rest   John Ladasky
• Desire recommendation re 2-D gel apparatus   John Ladasky
• pH for RNA work   Dr Banwari Lal
• Reference needed   Dr Banwari Lal
• Transposon mutagenesis   Dr Banwari Lal
• Available statistical programs   Mark Lamkin
• beads for cell culture   Jess H. Leber
• Test..Just Ignore   Chang-Muk Lee
• [Q] : insect hemocyte culture   Min-Ho Lee
• FISH protocols   Paul Lee
• FISH protocols   Paul Lee
• PCR and poly A sequences,   Wai Kwong Lee
• Transfecting CV1 cells   Victor Levenson
• Pfu polymerase in PCR   Victor Levenson
• Oligos: Who is cheapest?   Victor Levenson
• dam-/dcm- bugs and mutations??   Victor Levenson
• Scanalytics RFLPscan   David Ley
• skatron?   Nancy Linford
• Silver Staining Reveals Contaminating Bands in Sample B   David C. Logan
• Silver Staining Reveals Contaminating Bands in Sample   David C. Logan
• Antibody against GFP?   David C. Logan
• epitope mapping kit   Jimmy Loh
• Fusion Protein Expression Systems   LouPass
• Peptide nucleic acids (PNA)   Ludla
• Methods for in vitro mutagenesis   Kellie Ann Lugenbeel
• Protein Expression   Klaus Lun
• Protein Expression   Klaus Lun
• Need gp160 clones for HIV IIIB, MN, BRU   Mark Luscher
• High G/C PCR   Minh Luu
• multiplying bands in gel purification   John G. Luz
• Using a PC as an assay counter-timer...   R. John Lye
• blotting acrylamide gels   Constable Julie Lynn
• SEARLE/MONSANTO JOINS ELECTRONIC NOTEBOOKS CONSORTIUM   Lysakowski at aol.com
• SEARLE/MONSANTO JOINS ELECTRONIC NOTEBOOKS CONSORTIUM   Lysakowski at aol.com
• Image Analysis Shareware   Ted M.
• Na-acetate in RNA   RUTH MCMAHON
• Dissolve my pellet, please!   MELANSOND
• Smoking and smoke interactions with medications   MHPERTG at iris.rfmh.org
• Custom DNA-Sequencing in Europe   FABIAN MOEBIUS
• Geneskipper vs DNASIS   FABIAN MOEBIUS
• Oligos: Who is cheapest?   Dr. Michael T. MacDonell
• large amount of mRNA is needed   Rafael Maldonado
• Screening for Amp(s) bacteria.   Rafael Maldonado
• Stop codon read-through?   Rafael Maldonado
• Summary: Screening for Amp(s) bacteria.   Rafael Maldonado
• Transposon mutagenesis   Rafael Maldonado
• nonspecific primer extension products   Rafael Maldonado
• Restriction Enzymes in DNA Strider?   Harry Mangalam
• Chromosomal DNA carry-over in plasmid mini-preps   Michael Mangan
• Sequencing????   Darren Mark Manley
• PCR   Darren Mark Manley
• Restriction Enzymes in DNA Strider?   Ned Mantei
• Quick transformation protocol wanted   Jeremy Marcus
• Soluene-350 composition/source?   Mark
• roller bottle culture   Rolf Marteijn
• Disposal of Hoechst waste   Rolf Marteijn
• Oligos: Who is cheapest?   J. Pat Martinez
• differentiating between genomic & plasmid DNA   J. Pat Martinez
• HOW to Eliminate PCR primer dimer   Mary_Ann_Brow at TWT.COM
• does anyone have a spac-promoter vector   Neil Mattatall
• Which Email reader?   Matthew L. Brown, Ph.D
• Molecular biology software suggestions   Matthew L. Brown, Ph.D
• DNA analysis software   Matthew L. Brown, Ph.D
• 3'RACE PCR   Matthew L. Brown, Ph.D
• DNA sequence comparision   Matthew L. Brown, Ph.D
• A review on bacterial promoter consensus sequence   Jean-Francois Mayaux
• Hirt Extractions   Lawrence Mayer
• E.coli for Electroporation   Helen McBride
• Help   Guy McDonnell
• pLAFR1 cosmid restr. maps, anyone?   weezer Mccheese
• Wanted: Tie2 expressing cell line   Medvinsky
• quick question   Ulrich Melcher
• ssDNA (M13) extract. with silica?   Pascal Mertens
• Immunoblotting on colony lifts   Pascal Mertens
• pGL3 / pGL2 dilemma: anybody working with these ?   Dr. J. Miano
• Gene Finder   Dr. J. Miano
• bright specks in EtBr stained gels - out, damn'd spot!   Sam Michaelson
• Q: PCR cloning   Jan Michel
• 2mL Concave Gradients???   David Micklem
• DNA analysis software   Andrew Millar
• Q: purifying lambda phage from extraneous DNA   Paul Mitsis
• Molecular biology software suggestions   Debbie Moore
• <<test>> ignore   Glen Moore
• TEST -- ignore   Glen Moore
• Primer Design   Michael Morales
• digesting the ends of PCR products   David D Morgan
• fluorescent sequencing of cosmids   David D Morgan
• pH for RNA work   John Morlan
• Revs vs. g's in centrifugation   Mr. G. Morley
• quantifying small amounts of DNA   Mr. G. Morley
• Translational control   Mr. G. Morley
• WANTED: Src construct   Mr. G. Morley
• How much DNA can be "crowded" into a solution?   Ludvig Mortberg
• How many exonucleases have been discovered?   Ludvig Mortberg
• peptide hormone assays   Rory Morty
• fun with footprinting   Heidi Moss
• Growth and transformation of XL1-Blue   Kevin A. Mulcahy
• Calculation of transformation efficiency   Kevin A. Mulcahy
• Fragility of plasmids: size limit for shaking/vortexing?   Kevin A. Mulcahy
• Transfection of EBV-transformed B cell lines   Kevin A. Mulcahy
• Transient transfection of Cos-7 (electroporation v DEAE dextran)   Kevin A. Mulcahy
• plasmid DNA needed (50-100 mg)   Vincent Mulholland
• I can't get my multichannel pipette into my DNA engine.....:-(   Gordon Munro
• Phi 29 DNA polymerase   Gordon Munro
• T7 or SP6 RNA Pol promoter sequence   Gordon Munro
• pH for RNA work   Bernard Murray
• Transfecting CV1 cells   Bernard Murray
• subcloning large fragments   Bernard Murray
• Methods for in vitro mutagenesis   Bernard Murray
• GFP and FITC   Bernard Murray
• Screening for Amp(s) bacteria.   Bernard Murray
• Are there single letter codes for phosphoamino acids   Bernard Murray
• Has anyone tried SNuPE ?   Bernard Murray
• DNA recovery by phenol/chloroform extraction or GeneClean   Bernard Murray
• translational control ??   Bernard Murray
• northern blotting   Bernard Murray
• quick question   Bernard Murray
• Help: cleaning sintered glass funnels   Bernard Murray
• quick question   Bernard Murray
• Dexamethasone-inducible pMAMneo vector   Bernard Murray
• Enhancement of HRP substrate systems??   Per Mygind
• paraformalduhyde fixation and membrane integrity   Tsunehisa Namba
• luciferase   Your Name
• Revs vs. g's in centrifugation   Darren Natale
• Efficient method for analysis of recombinants?   Greg Nattrass
• High G/C PCR   Greg Nattrass
• Q: Clones from genomic libraries?   Bart Nelissen
• Room Sharing at 41st Biophysical Meeting   Newswatcher
• epitope tagging   Toai T. Nguyen
• E.Coli Plasmid<--->Chromosome Insertion/Recovery?   Brad Nicholson
• Transposon mutagenesis   Brad Nicholson
• Transient transfection of Cos-7 (electroporation v DEAE dextran)   Rick Nicholson
• A review on bacterial promoter consensus sequence   Gerd Nilsen
• Phenol Protocol   Alexandre Noël
• Boehringer DIG system experiences   Martin Offterdinger
• Growth and transformation of XL1-Blue   Martin Offterdinger
• Error rate in Taq pol. 1:270?   Martin Offterdinger
• DNA-free RNA   Martin Offterdinger
• SV40 poly(A) signal works bidirectionally?   Hiroyoshi Ohba
• pEE14   Bjšrn Olde
• A little help here...   The Kindly One
• screening of cDNA library: custom services   Myriam Onno
• Antisense oligonucleotides   Sandra Pena De Ortiz
• Help: cleaning sintered glass funnels   Guy Oshiro
• TEST   HARIHARAN P.EASWARAN
• Want to hook DNA/RNA to solid matrix   HARIHARAN P.EASWARAN
• Washing slides for in situs   GILLIAN NI BHARLO BOTANY PG
• GCG   Gurcharan Pahwa
• Reference needed   Elise Palmer
• Horseradish Peroxidase assay   Tom Parish/Cambridge/Biogen
• Molecular Modellers - Wanted   Michael Parkinson
• nylon mesh for hybridizations   Derek Parsonage
• Immunoblotting on colony lifts   Stephane Pasteau
• dot blot with bacteria   Stephane Pasteau
• VHD strain request   Stephane Pasteau
• transgenic bacteria   Stephane Pasteau
• Na-acetate in RNA   Chris Patil
• Question: Cytoplasmic and nuclear fractionation of cells.   John Pawlowski
• Primer Design   Mirna Perez
• Which quantitative RT-PCR?   Linda Perry
• Aliquoting Antibodies for freezing - HELP!   Pete
• electrophoresis at pH 8,0   Peter
• (none)   Matthew David Petroski
• ZAP systems   Andy Phillips
• Preparation of rifampicine   C E Pical
• Looking for cDNA sequence or accession number of IRS-2   Peter Pichler
• Looking for cDNA sequence or accession number of IRS-2 - whole text   Peter Pichler
• Pins microplates cover   Grognet Pierre
• Smearing of PCR product   Landry Pierre-Alexandre
• removal of PEG from DNA disolved in TE?   Venkat Pisupati
• Fusarium & NORTHERN BLOTS   Cristina Placinta
• Quick transformation protocol wanted   Eyck Poetter
• GST-fusion protein extraction   Philippe Pognonec
• Which Email reader?   Michael J. Prigge
• Hot plasmid + Cold Vector = Insert-specific probe????   Michael J. Prigge
• Gradient Thermocyclers   Kiley R. Prilliman
• Transfection of EBV-transformed B cell lines   Kiley R. Prilliman
• quick question   Kiley R. Prilliman
• multiplying bands in gel purification   QIAGEN
• ATP   SARAH LOUISE RAWLINGS
• Source of bismuth subcitrate in N. America?   REW
• inositol phosphate enzymatic hydrolysis   Michel RIVIERE
• 48-Well microplates   RManns7
• Pins microplates cover   RManns7
• subscription to a newsgroup   Francis ROBERT
• Stratagene QuikChange mutagenesis?   Keith Rand
• Quick transformation protocol wanted   Colin Rasmussen
• Sectioning mouse eyes Help!   Kevin Rattray
• Checking labelling oligo   Marcus Rattray
• Human cDNA libraries for two-hybrid system   Sanjeeva Reddy
• Molecular clone of dehydroepiandrosterone   Mary P. Remington
• pAb against GFP / EGFP   Gunther Rezniczek
• SEARLE/MONSANTO JOINS ELECTRONIC NOTEBOOKS CONSORTIUM TO DEVELOP SYSTEMS TO PROTECT AND MANAGE INTELLECTUAL PROPERTY RECORDS FOR R&D   Rich Lysakowski, Ph.D.
• SEARLE/MONSANTO JOINS ELECTRONIC NOTEBOOKS CONSORTIUM TO DEVELOP SYSTEMS TO PROTECT AND MANAGE INTELLECTUAL PROPERTY RECORDS FOR R&D   Rich Lysakowski, Ph.D.
• DNA-based assay of apoptosis needed   Robert W. St. G. Fisher, IV
• Pierce ECL and BioRad's phosphoimager system?   Robert W. St. G. Fisher, IV
• beads for cell culture   Robertoalf
• DNA-based assay of apoptosis needed   Julie Roberts
• Which Email reader?   Julie Roberts
• Oligo problems   Julie Roberts
• anti-prion protein   Julie Roberts
• best total RNA and mRNA isolation kits   Julie Roberts
• Q:Measurement of protein's surface hydrophobicity?   Dr S. Robertson
• Cryostat sectioning...   Jen Robertson
• Protein Labeling? Casein Kinase?   Phillip Robinson
• what kind of film?   Phillip Robinson
• Transfecting CV1 cells   Lourdes Rodgers
• Help! PCR linearity   Joanne Rogers
• Help! PCR linearity   Joanne Rogers
• Anyone Using Competimer (TM) Technology for RT-PCR?   Richard Rohan
• Problems with synthetic gene synthesis   Gijs van Rooijen
• Problems with synthetic gene synthesis (Summary of replies)   Gijs van Rooijen
• painful gel loading???!!!!!!   Mark S. Rose
• RNA extraction from little tissue.   Hiranya Roychowdhury
• Q: inducible promoters for plants   Hiranya Roychowdhury
• Q: inducible promoters... retraction!!   Hiranya Roychowdhury
• Q: purifying lambda phage from extraneous DNA   Hiranya Roychowdhury
• RNA extraction from small samples...   Hiranya Roychowdhury
• in search of "Stains-All" staining protocol   Hiranya Roychowdhury
• differentiating between genomic & plasmid DNA   Hiranya Roychowdhury
• quick question   Hiranya Roychowdhury
• PVP/PVPP   Hiranya Roychowdhury
• Gridded Membranes   Hiranya Roychowdhury
• Questions about DNA recovery and cloning   Hiranya Roychowdhury
• pH for RNA work   George Rutherford
• Dissolve my pellet, please!   George Rutherford
• contamination of glycine by other amino acids ???   George Rutherford
• Boehringer DIG system experiences   Ed Rybicki
• (none)   Takeshi SAITO
• DNA analysis software   WILL H SCOVILL
• Image analysis help needed   SCROOD
• Smearing of PCR product   XIAO-MIN SU
• Image Analysis Shareware   Juha Saharinen
• Which Email reader?   Juha Saharinen
• sources of hsp90?   Sai
• Probing of an expression library with oligopeptides.   Sai
• DNA recovery by phenol/chloroform extraction or GeneClean   Sai
• rubidium chloride for making competent cells?   Sai
• 3' RACE PC   Dr. N.A. Salazar
• Stripping Northerns   Klaus Salger
• bright specks in EtBr stained gels - out, damn'd spot!   Klaus Salger
• Northern blot RNA degradation   Klaus Salger
• Kozak concensus sequence   Deborah A. Samac
• Electroelution   Steven Sanyal
• Amplification from a single algal colony, any thoughts?   Airlie Sattler
• fos messenger size   W. Schechinger
• fos mRNA size   Wolfgang Schechinger
• stability of GFP in E.coli?   Wolfgang Schechinger
• Disposal of Hoechst waste   Wolfgang Schechinger
• HELP:PAGE Probes   Wolfgang Schechinger
• Adhesive temperature indicator / warning for reagants & buff   Wolfgang Schechinger
• Looking for cDNA sequence or accession number of IRS-2   Wolfgang Schechinger
• HELP:PAGE Probes   Steve Scherer
• N-terminal sequence   Emile Schiltz
• SSC conc. in prehyb.   Denni Schnapp
• Tris base vs. Trizma   Denni Schnapp
• Q:Measurement of protein's surface hydrophobicity?   "Uncle Al" Schwartz
• Trcky vectors   Ed Schwartz
• WANTED: pTrc99 plasmids   Ed Schwartz
• DNA-quantitation   Joern Schwede
• DNA extract with CTAB   Brendan Scott
• Cloning problem   Brendan Scott
• Cloning problem   Brendan Scott
• Conductance Standards   Leon Scott
• Streptavidin and Biotin interactions   John Richard Seavitt
• Expression of 2 proteins from different vectors   Bret Sellman
• retroviral particle size?   Jurgen Seppen
• beads for cell culture   Jurgen Seppen
• multiplying bands in gel purification   Jurgen Seppen
• **************Gancyclovir**********************   Jurgen Seppen
• e.mail subscription   Rubina Shafi
• meaning of acronym   Regina Shaw
• XGAL & IPTG -- how low can you go?   Glen Shearer
• pH dependence of DNA hydrolysis   Shuiliang Shi
• Q: How doing partial RE digests?   Sanjay Shirke
• bright specks in EtBr stained gels - out, damn'd spot!   Rich Showalter
• The Antibody Resource Page   Kevin Shreder
• Q: Source of terminating ribonucleotides   Igor Sidorenkov
• Custom DNA-Sequencing in Europe   H.J. Sluiman
• High G/C PCR   Dr Koen A.L. De Smet
• degenerate PCR   Dr Koen A.L. De Smet
• how much lambda vector do I need to use   Ken Soderstrom
• Where to buy E. coli NP66 for in vivo phagemid release   Christian P. Sommerhoff
• how to identify a gene on human chromsome 11   Soonmyoung Paik, M.D.
• RNA Splicing Software   Soonmyoung Paik, M.D.
• cleaning pipettes   Keld Sorensen
• 48-Well microplates   Keld Sorensen
• Non-specific binding of avidin woes.   Keld Sorensen
• Non-specific binding of avidin woes.   Keld Sorensen
• Pierce ECL and BioRad's phosphoimager system?   Keld Sorensen
• Help: cleaning sintered glass funnels   Keld Sorensen
• Na-acetate in RNA   David F. Spencer
• Disposal of Hoechst waste   David F. Spencer
• FOA problems-Help   John Stebbins
• Q re: PCR from agarose plugs   Bob Steinberg
• Pfu polymerase in PCR   Bob Steinberg
• refolding from inclusion bodies   Bob Steinberg
• RER+ mouse cell lines   Bob Steinberg
• Sequencing and sharkteeth   Jørgen Stenvik
• Fusion Protein Expression Systems   Steve
• 220 RNA peak   Wendy Stewart
• Request for pUC7   Miss S.L. Stowell
• PCR contamination   Stsarev
• Q: purifying lambda phage from extraneous DNA   Greg Stuart
• My biology site (fwd)   Sudeesha Kunjibettu, by way of C. S. Prakash
• Q re: PCR from agarose plugs   Steven Sullivan
• Which Email reader?   Dennis Summerbell
• radioactive actinomycin D   William Sun
• myelin   Sandra Supraha
• myelin   Sandra Supraha
• Luciferase   Yuriko Suzuki
• hot PCR   Caroline Szymeczek-Seay
• Number of Bacteria per plate?   TJ
• Deep Vent Pol (ssDNA)   Moez TORKI
• Anyone have a source for bovine cTnT?   Jil Tardiff
• mAb Purification Difficulties   Tom Thatcher
• Probing of an expression library with oligopeptides.   Thomas
• MMLV and rNTP's   Matt Thomas
• RNA Ligase   Matt Thomas
• Measuring DNA Concentration: UV absorption shift?   Yvonne Thorstenson
• Using a PC as an assay counter-timer...   Richard T. Timmer
• Using a PC as an assay counter-timer...   Richard T. Timmer
• Protein-protein interaction of His-tag of pET14b?   Jimmy S.H. Tsang
• antigenic algorythms software/Fusion prot.s   Ross Turbyfill
• epitope mapping kit   Ross Turbyfill
• Monoclonal vs polyclonal antibody   Ross Turbyfill
• RNase Protection Assay   Antonin Tutter
• **************Gancyclovir**********************   Przemko Tylzanowski
• Revs vs. g's in centrifugation   Darren Tyson
• fos mRNA size   Darren Tyson
• 2D peptide mapping   Darren Tyson
• protein's pI   Darren Tyson
• oligo biotinylation?   Darren Tyson
• address   STEEVE VERONNEAU
• bright specks in EtBr stained gels - out, damn'd spot!   Jeff Velten
• FITC-Guard source?   Richard Vernell
• Random peptide library   Richard Vernell
• Transient transfection of Cos-7 (electroporation v DEAE dextran)   Steeve Veronneau
• best total RNA and mRNA isolation kits   Beisvåg Vidar
• Strange contaminant in cell culture   Tom Vink
• RNA secondary structure   Tom Vink
• cleaning pipettes   David Voduc
• Mycoplasm test by PCR   S Von
• Reamplification pcr products after Ag-staining   Michael Vork
• Emission wavelength of EtBr/DNA for a CCD camera filter   Karl Voss
• Tris base vs. Trizma   Karl Voss
• Restriction Enzymes in DNA Strider?   Mark Walberg
• Test Post   Ed Wang
• Vol of ligation mix to use in transformation   Keith Warner
• Which Email reader?   John Watson
• RNA from tiny amounts of tissue?   John Watson
• Pfu polymerase in PCR   John Watson
• Cloning problem   John Watson
• 3'RACE PCR   John Watson
• Reamplification pcr products after Ag-staining   John Watson
• Primer design   John Watson
• quick question   John Watson
• blotting acrylamide gels   John Watson
• Streptavidin and Biotin interactions   Diane Webster
• TRAP ASSAY   Xin Wei
• Q: How to remove small size RNA from total RNA in bacteria?   Chi-kuang Wen
• Na-acetate in RNA   Chi-kuang Wen
• SSC conc. in prehyb.   Chi-kuang Wen
• pH of water; dissolving RNA   Chi-kuang Wen
• 5'-psoralen-labelled primers   Karel Wernars
• Protein Extraction Buffers...What's a good homogenization buffer for westerns?   Rob Wesley
• Pierce ECL and BioRad's phosphoimager system?   Ann L. Williams
• XGAL & IPTG -- how low can you go?   Ann L. Williams
• Smearing of PCR product   Ann L. Williams
• Ecdysone Inducible System   Chris Williams
• INDEX MEDICUS address   Jenny Williams
• PYMS Diagram   Jenny Williams
• Help!Mouse fibroblast cell line dying!   Tori D. Williams
• protein's pI   Randy Willis
• Fusion Protein Expression Systems   Randy Willis
• E. coli (Bl21DE3) lyses automatically   Randy Willis
• Protein Expression   Randy Willis
• refolding from inclusion bodies   Randy Willis
• Sequencing and sharkteeth   Randy Willis
• A BiaCore-like system   Randy Willis
• DNA recovery by phenol/chloroform extraction or GeneClean   Wilson
• DNA recovery by phenol/chloroform extraction or GeneClean   Wilson
• DNA recovery by phenol/chloroform extraction or GeneClean   Wilson
• Questions about DNA recovery and cloning   Wilson
• Question about genomic library construction   Wilson
• Question about genomic library construction   Wilson
• Question about genomic library construction   Wilson
• Spectrophotometers   Karl A. Wilson
• Methods for in vitro mutagenesis   Troels Wind
• Pins microplates cover   Troels Wind
• Stratagene QuikChange mutagenesis?   Troels Wind
• How to purify periplasmic fraction?   Troels Wind
• beads for cell culture   Troels Wind
• Quick transformation protocol wanted   Troels Wind
• bright specks in EtBr stained gels - out, damn'd spot!   David Winterbourne
• Q: purifying lambda phage from extraneous DNA   David Winterbourne
• Long PCR & Joining 5' of sequence to the rest   Harry Witchel
• Helix pomatia cDNA   Ute Wittstock
• Oligo problems   Arthur Wohlwill
• mAb Purification Difficulties   Walter Woith
• bright specks in EtBr stained gels - out, damn'd spot!   Walter Woith
• Restriction Enzymes in DNA Strider?   Jim Woodgett
• yeast two hybrid   R. Woodward
• Na-acetate in RNA   Laura Woody
• How to use BLAST   Zhencai Wu
• Wanted: Tie2 expressing cell line.   XXX
• Q: How to remove small size RNA from total RNA in bacteria?   Jinling Xie
• Q: How to remove small size RNA from total RNA in Bacteria?   Jinling Xie
• Q: PCR cloning   Jinling Xie
• PCR product into northern probe ??   Youhua Xie
• "Oligonucleotide purification method??"   Youhua Xie
• cDNA product separation   Yang Y.L.
• RT-PCR question !   Yang Y.L.
• translational control ??   Yang Y.L.
• Primer design   Yang Y.L.
• [Q]What is PMA converted to ?   Hisanori Yamamoto
• How to separate vegf dimer into monomer?   Ziping Yang
• pH for RNA work   H. Yim
• does anyone have a spac-promoter vector   H. Yim
• Fusion Protein Expression Systems   Ian A. York
• Low RNA yield after Qiaquick column purification.   Zhongtang Yu
• beads for cell culture   Zhongtang Yu
• LPS-free DNA   Sujeong Yun
• Laboratory Robotics Interest Group meeting   Andy Zaayenga
• How to purify periplasmic fraction?   Hayssam Zakaria
• pGL3 / pGL2 dilemma: anybody working with these ?   Alt Zantema
• Calculation of transformation efficiency   Marieke R. Koedood Zhao
• differentiating between genomic & plasmid DNA   Marieke R. Koedood Zhao
• Limited digestion   Marieke R. Koedood Zhao
• RNase Protection Assay   Marieke R. Koedood Zhao
• Antisense transcripts   Zhiyong Zhao
• test   XiaoYin Zhong
• (none)   Zhuang
• 48-Well microplates   Alfred Zimmerlin
• northern blotting   a.mccormick
• Help suppliers of biotinylated magnetic beads   a.molloy
• roller bottle culture   shanthi adimoolam
• Two-Hybrid System for E coli ?   ajduclos at NEXUS.MICROIMM.MCGILL.CA
• Deglycosylation of proteins: questions   hadi al-hasani
• GFP and FITC   eric anderson
• I need graphics of the number of IgG (y) in function of time (x)   ballet at enib.fr
• pCR-Blunt vs pCR-Script   bernielu at ERE.UMontreal.CA
• vector pMT22   anne borycki
• learning to use newsgroup   boukhb01 at mcrcr6.med.nyu.edu
• subcloning large fragments   brett
• Calculation of transformation efficiency   brett
• Cleaning up Transcriptions   brett
• Transfection   brett
• Calcium Phosphate Precipitation   brett
• Kozak concensus sequence   brett
• acid phenol extractions   brett
• Strange contaminant in cell culture   brett
• optimizing RT-PCR?   Stefano casalotti
• epitope tagging background in plants   chen0183 at maroon.tc.umn.edu
• antigenic algorythms software/Fusion prot.s   darren cikaluk
• (none)   cjvs at PUBLIC.CC.JL.CN
• Effect of Phenol degradation prodcust on bacterial bioluminescence   corbisip at vito.be
• recombinant protein labelling with methionine   curmi at infobiogen.fr
• 3'RACE PCR   czlq at musica.mcgill.ca
• protein's pI   czlq at musica.mcgill.ca
• AATAAA signal   czlq at musica.mcgill.ca
• PCR   czlq at musica.mcgill.ca
• refolding from inclusion bodies   bipin k. dalmia
• Error rate in Taq pol. 1:270?   derk at hubrecht.nl
• nucler extract   doering_t at mpimg-berlin-dahlem.mpg.de
• Heparin in shift assays   doering_t at mpimg-berlin-dahlem.mpg.de
• in search of "Stains-All" staining protocol   doering_t at mpimg-berlin-dahlem.mpg.de
• DNA-free RNA   orlando dominguez
• Tris base vs. Trizma   ege
• Co-immunoprecipitation of nuclear proteins   ege
• Thermotoga maritima DNA/cells   alan friedman
• Best commercial Factor Xa?   alan friedman
• single tube nested pcr   gzk at ollamh.ucd.ie
• guanylyl cyclase antibody   m hayden
• LB contains glucose?   hbkg at hitel.kol.co.kr
• Primer design strategy?   hcorbett at garnet.berkeley.edu
• direct genomic sequencing   hendrikb
• mutation analysis   hendrikb
• large amount of mRNA is needed   hendrikb
• Smearing of PCR product   xudong huang
• pCYPAC2   jcorral at gm.iro.es
• sequencing signal dies after 100 bases?!   jfh
• sequence data anomaly-PCR clone   jmorrey
• Transposon mutagenesis   john.sokatch at CCLINK.NET.UOKHSC.EDU
• Kozak concensus sequence   jovermeyer at shrsys.hslc.org
• Co-immunoprecipitation of nuclear proteins   jstrassw at OPAL.TUFTS.EDU
• refolding from inclusion bodies   juhi juneja
• WANTED: Human Genomic (Cosmid) Library   jxie at bi-pharm.com
• microsatellite loci amplification   kfeldh1 at ICARUS.CC.UIC.EDU
• Need tips for sequencing through poly-c tail   ku at helix.nih.gov
• unsubscribe   kwilson at DARWIN.UCSC.EDU
• Help on bacterial analysis?   l95mn at river.tay.ac.uk
• S.aureus primers   laban
• Subtractive Probe   charles lawrie
• Q:Measurement of protein's surface hydrophobicity?   lgbell at liverpool.ac.uk
• Na+/K+ ATPase assay   lincoln at indigo.ie
• Need gp160 clones for HIV IIIB, MN, BRU   luscher at immune.med.utoronto.ca
• Immunohistochemistry help   melvnr at hg.uleth.ca
• Calcium Phosphate Precipitation   mennow at ruly46.medfac.leidenuniv.nl
• RNase Protection Assay   mennow at ruly46.medfac.leidenuniv.nl
• painful gel loading???!!!!!!   natldiagnos at mindspring.com
• bright specks in EtBr stained gels - out, damn'd spot!   natldiagnos at mindspring.com
• Dissociating agents to prevent protein aggregation   newera at plaza.snu.ac.kr
• mRNA isolation kits for plant culture tissue   nhwu at PUBLIC3.BTA.NET.CN
• RNA from urine   nik
• CapFinder PCR cDNA library construction kit   swathi padmanabhan
• electrophoresis units recommendations?   peter
• subcloning large fragments   pmiguel at bilbo.bio.purdue.edu
• Jurkatt cell induction   rayj
• Mini Mu bacteriophage   albano renee
• anti-prion protein   sas96igg at rdg.ac.uk
• Reamplification pcr products after Ag-staining   rebecca slayton
• Calcium Phosphate Transient Transfection of NIH/3T3???   john storring
• Oligos: Who is cheapest?   cynthia stryker
• DNA curvature software   subtilis
• Circular permutation assay   subtilis
• DNAcleaner, french laboratories already use it against contaminations.   sunfish
• Problems with One Shot INValphaF' from Invitrogen   sunfish
• Problems with One Shot INValphaF' from Invitrogen   sunfish
• acid phenol extractions   sunfish
• Electroelution   taguebw at wfu.edu
• Electroelution   taguebw at wfu.edu
• Electroelution   taguebw at wfu.edu
• Pfu polymerase in PCR   test
• Low infection of WEHI231 cell line with eco/ampho retrovirus   tim
• anti-vinculin antibody   u.381 at inserm.u-strasbg.fr
• PCR product into northern probe ??   u8251012 at cc.kmc.edu.tw
• HOW to Eliminate PCR primer dimer   u8251012 at cc.kmc.edu.tw
• How can I assay translational rate ??   u8251012 at cc.kmc.edu.tw
• Kodak Biomax Vs Fuji RX   frogs 'r' us
• A freezer inventory software?   vcore
• (none)   vduke at ANCON.UP.AC.PA
• (none)   vduke at ANCON.UP.AC.PA
• multiplying bands in gel purification   vilimf01 at mcrcr6.med.nyu.edu
• removal of PEG from DNA disolved in TE?   vilimf01 at mcrcr6.med.nyu.edu
• ?-easiest clean-up for sequencing   vilimf01 at mcrcr6.med.nyu.edu
• Making Nickel resin??   wegloehner at mpimg-berlin-dahlem.mpg.de
• TRAP assay   xin wei
• protein phosphorylation   wellman
• DNA sequence comparision   wjc at acsu.buffalo.edu
• expression vector problem   wliu at receptor.pharm.uwa.edu.au
• PCR and poly a sequences. - wai1.doc [1/2]   wlkl1j at clinmed.gla.ac.uk
• Help with PCR adding sp6 sequence   william wu
• beads for cell culture   xxx
• Stratagene Site-directed mutagenesis   zxiong at ag.arizona.edu
• Gene organization   Sigurd Ørstavik

Last message date: Fri Feb 28 22:10:36 EST 1997
Archived on: Sun Mar 6 21:22:10 EST 2005

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