Methods February 1997 Archives by date

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Starting: Sat Feb 1 00:52:13 EST 1997
Ending: Fri Feb 28 22:10:36 EST 1997
Messages: 1105

SYPRO Orange Quenching by 35-S Dick Haugland
PCR product into northern probe ?? u8251012 at cc.kmc.edu.tw
pGL3 / pGL2 dilemma: anybody working with these ? Dr. J. Miano
Gene Finder Dr. J. Miano
SV40 poly(A) signal works bidirectionally? Hiroyoshi Ohba
digesting the ends of PCR products Martin Hughes
MMLV and rNTP's Matt Thomas
Oligo problems Arthur Wohlwill
Short seq search? Chihara
PCR product into northern probe ?? Youhua Xie
Question: Cytoplasmic and nuclear fractionation of cells. John Pawlowski
cDNA product separation Yang Y.L.
RT-PCR question ! Yang Y.L.
Hydropathy plot software wanted Oliver S. DePeyer
Need tips for sequencing through poly-c tail Scott Hodgson
HOW to Eliminate PCR primer dimer u8251012 at cc.kmc.edu.tw
(no subject) Joan Boyce
Monoclonal CAT Joan Boyce
Boehringer DIG system experiences Karen Ferri
HOW to Eliminate PCR primer dimer Huang Ke-xue
Protein Labeling? Casein Kinase? Thomas J. Albert
Image Analysis Shareware Ted M.
Image analysis help needed SCROOD
Need tips for sequencing through poly-c tail ku at helix.nih.gov
Emission wavelength of EtBr/DNA for a CCD camera filter Dr. Duncan Clark
Boehringer DIG system experiences Georg Kroeger
Q: How doing partial RE digests? Sanjay Shirke
Vol of ligation mix to use in transformation Keith Warner
Thermotoga maritima DNA/cells alan friedman
HOW to Eliminate PCR primer dimer Natalie Bloch
Neomycin/Kanamycin Resistance C. J. Fields
Q: inducible promoters for plants Dr. K. Eimert
Boehringer DIG system experiences Martin Offterdinger
Boehringer DIG system experiences Mike Challen
Which Email reader? Graham Atherton
Subtractive Probe charles lawrie
Protein Labeling? Casein Kinase? Phillip Robinson
Phenol Protocol Alexandre Noël
RNA standards for quantitative PCR Adrian Allan
Selling a used Beckmann rotor Roger C. Green
pH dependence of DNA hydrolysis Shuiliang Shi
Q re: PCR from agarose plugs Steven Sullivan
Vol of ligation mix to use in transformation Karl Fischer
PCR primer-dimer elimination Robert B. Chadwick
HPRT targeting vector Constanze Bonifer
Phenol Protocol David L. Haviland, Ph.D.
roller bottle culture shanthi adimoolam
TEST HARIHARAN P.EASWARAN
P.E. 9600 thermocyclers-the cooling fix... Christopher G. Alpha
Growth and transformation of XL1-Blue Kevin A. Mulcahy
Image Analysis Shareware Dima Klenchin
DGGE Gel Drying...? Dr Steve Griffiths Molecular Biology Group
pH for RNA work Dr Banwari Lal
digesting the ends of PCR products Steven Goldberg
Transfecting CV1 cells Lourdes Rodgers
Pointer to FAQ lists Paul N Hengen
Membrane preps Brenda
Want to hook DNA/RNA to solid matrix HARIHARAN P.EASWARAN
cleaning pipettes David Voduc
Help with PCR adding sp6 sequence william wu
digesting the ends of PCR products Ashok Aiyar
roller bottle culture Rolf Marteijn
Primer Design Mirna Perez
Subtracted cDNA Probe Barbara
Emission wavelength of EtBr/DNA for a CCD camera filter Karl Voss
Which Email reader? Matthew L. Brown, Ph.D
DNA extract with CTAB Brendan Scott
Cloning problem Brendan Scott
Q re: PCR from agarose plugs Bob Steinberg
Number of Bacteria per plate? TJ
DNA-free RNA Francois Hatey
pH for RNA work John Morlan
Transfecting CV1 cells Dima Klenchin
Sigma Monoclonal Anti-polyHis David Ackerley
Protein Labeling? Casein Kinase? Thomas J. Albert
pH for RNA work Bernard Murray
Transfecting CV1 cells Bernard Murray
DNA-based assay of apoptosis needed Jerry Kropp
Quntifying small amounts of DNA reliably Natalie Bloch
FISH protocols Paul Lee
FISH protocols Paul Lee
Primer Design Natalie Bloch
I can't get my multichannel pipette into my DNA engine.....:-( Gordon Munro
HOW to Eliminate PCR primer dimer Frank O. Fackelmayer
sequencing signal dies after 100 bases?! Jan Hoolwerf
cleaning pipettes Keld Sorensen
Image Analysis Shareware Juha Saharinen
Boehringer DIG experiences Andrew Bettany
Preparation of rifampicine C E Pical
Na-acetate in RNA RUTH MCMAHON
Which Email reader? Joao V. Costa
peptide hormone assays Rory Morty
I need more infor about versene Richard P Grant
Calculation of transformation efficiency Kevin A. Mulcahy
Growth and transformation of XL1-Blue Fraser Dr N J
Transfecting B cells Erik Kul| Hagen
HOW to Eliminate PCR primer dimer Hedley Carr
E.Coli Plasmid<--->Chromosome Insertion/Recovery? Mark Blight
I need more infor about versene David L. Haviland, Ph.D.
Q: How doing partial RE digests? Mark Blight
Calculation of transformation efficiency David L. Haviland, Ph.D.
Imidazole buffer has esterase activity? Steven Goldberg
Which Email reader? Labor 1
Restriction Enzymes in DNA Strider? Namjin Chung
GST-fusion protein extraction Philippe Pognonec
XGAL & IPTG -- how low can you go? Glen Shearer
Molecular biology software suggestions Debbie Moore
Primer Design Michael Morales
Growth and transformation of XL1-Blue Alexander N. Kukushkin
Calculation of transformation efficiency Marieke R. Koedood Zhao
Need tips for sequencing through poly-c tail
Km calculation web site Huang Ke-xue
DNA-based assay of apoptosis needed Jerry Kropp
Boehringer DIG system experiences Ed Rybicki
220 RNA peak Wendy Stewart
Dissolve my pellet, please! Josh Berke
subcloning large fragments Frederic G. Barr Lab
pH for RNA work George Rutherford
Molecular biology software suggestions Matthew L. Brown, Ph.D
Which Email reader? Michael J. Prigge
GFP and FITC Danny Altschuler
retroviral particle size? Danny Altschuler
subcloning large fragments brett
Calculation of transformation efficiency brett
contamination of glycine by other amino acids ??? Queen of Molecular Biology
Restriction Enzymes in DNA Strider? Harry Mangalam
subcloning large fragments David L. Haviland, Ph.D.
subcloning large fragments pmiguel at bilbo.bio.purdue.edu
Measuring DNA Concentration: UV absorption shift? Yvonne Thorstenson
radioactive actinomycin D William Sun
How to perform a DNA Methyltransferase assay? Francisco J. Alarcon
Q: How doing partial RE digests? Lee Fradkin
retroviral particle size? Dima Klenchin
Need to find Supplier of Anti-Neo Blair Jennings
I need more infor about versene Forget-me-not
Article Search Martin Beaulieu
subcloning large fragments Bernard Murray
Dissolve my pellet, please! George Rutherford
contamination of glycine by other amino acids ??? George Rutherford
Growth and transformation of XL1-Blue Martin Offterdinger
test - please ignore Granny
DNA extract with CTAB Tim Barnett
High G/C PCR Dr Koen A.L. De Smet
HDC antibody Yoshitaka Konda
(none) Zhuang
nucler extract doering_t at mpimg-berlin-dahlem.mpg.de
Heparin in shift assays doering_t at mpimg-berlin-dahlem.mpg.de
Efficient method for analysis of recombinants? Greg Nattrass
High G/C PCR Greg Nattrass
How much DNA can be "crowded" into a solution? E.Hillery
Fragility of plasmids: size limit for shaking/vortexing? Kevin A. Mulcahy
electrophoresis at pH 8,0 Peter
DNA-based assay of apoptosis needed Julie Roberts
Calculation of transformation efficiency Alexander N. Kukushkin
DNA curvature software subtilis
Revs vs. g's in centrifugation Mr. G. Morley
Cloning problem Nick Jacobsen
Helix pomatia cDNA Ute Wittstock
<<test>> ignore Glen Moore
SV40 poly(A) signal works bidirectionally? Alan KAY
Methods for in vitro mutagenesis Kellie Ann Lugenbeel
fos messenger size W. Schechinger
Emission wavelength of EtBr/DNA for a CCD camera filter Dr. Duncan Clark
digesting the ends of PCR products David D Morgan
fos mRNA size Wolfgang Schechinger
How much DNA can be "crowded" into a solution? Ludvig Mortberg
pH for RNA work H. Yim
Trcky vectors Ed Schwartz
Which Email reader? John Watson
pH for RNA work Shi-Jye Chu
Luciferase Yuriko Suzuki
Fragility of plasmids: size limit for shaking/vortexing? Alexander Kraev
Na-acetate in RNA Chris Patil
TEST -- ignore Glen Moore
Revs vs. g's in centrifugation Darren Natale
mAb Purification Difficulties Patricia Keely
pH for RNA work David L. Haviland, Ph.D.
E.Coli Plasmid<--->Chromosome Insertion/Recovery? Michael Benedik
retroviral particle size? Jurgen Seppen
Tris base vs. Trizma ege
GST-fusion protein extraction Cornelius Krasel
sources of hsp90? Sai
Methods for in vitro mutagenesis Bernard Murray
RNA from tiny amounts of tissue? John Watson
recombinant protein labelling with methionine curmi at infobiogen.fr
Revs vs. g's in centrifugation Darren Tyson
fos mRNA size Darren Tyson
Calcium Phosphate Transient Transfection of NIH/3T3??? john storring
E.coli for Electroporation Helen McBride
large amount of mRNA is needed Rafael Maldonado
RNA extraction from little tissue. Hiranya Roychowdhury
Pfu polymerase in PCR Bob Steinberg
digesting the ends of PCR products AMICVS CONSTANTINI
Crustacean ITS Phylogeny and PCR Ari Kahn
Co-immunoprecipitation of nuclear proteins Richard Dahl
Electroelution Steven Sanyal
GFP and FITC Dick Haugland
imidazole buffer has esterase activity Dick Haugland
RNA from tiny amounts of tissue? Alex Dobrovic
Restriction Enzymes in DNA Strider? Ned Mantei
GFP and FITC Andrew Doherty
bright specks in EtBr stained gels - out, damn'd spot! David Winterbourne
DNA SOFTWARE INFORMATION PLEASE CLARKEY
imidazole buffer has esterase activity Zophonias O. Jonsson
Deep Vent Pol (ssDNA) Moez TORKI
Probing of an expression library with oligopeptides. CLARKEY
Which Email reader? Gabriele Kerber
mAb Purification Difficulties Walter Woith
Restriction Enzymes in DNA Strider? Mark Walberg
Pins microplates cover Grognet Pierre
Quntifying small amounts of DNA reliably Gerome Breen
bright specks in EtBr stained gels - out, damn'd spot! Walter Woith
Qustion on Ammonium sulfate and other precipitation methods Cornelius Krasel
Electroelution taguebw at wfu.edu
Electroelution taguebw at wfu.edu
Electroelution taguebw at wfu.edu
Which Email reader? - Results Graham Atherton
Dissolve my pellet, please! MELANSOND
Pfu polymerase in PCR Fraser Dr N J
quantifying small amounts of DNA Mr. G. Morley
[Q]What is PMA converted to ? Hisanori Yamamoto
Methods for in vitro mutagenesis Troels Wind
Which Email reader? Dennis Summerbell
Pins microplates cover Troels Wind
antigenic algorythms software/Fusion prot.s darren cikaluk
Q: inducible promoters for plants Curt Ashendel
paranitrophenyl acetate Bernard Dumas
Which Email reader? Julie Roberts
Q: inducible promoters for plants Hiranya Roychowdhury
DNA Sequencing DSF
DNA-based assay of apoptosis needed Robert W. St. G. Fisher, IV
220 RNA peak Shelley Cole
bright specks in EtBr stained gels - out, damn'd spot! Sam Michaelson
High G/C PCR Minh Luu
Probing of an expression library with oligopeptides. Sai
Which Email reader? Louis Hom
Q: inducible promoters... retraction!! Hiranya Roychowdhury
bright specks in EtBr stained gels - out, damn'd spot! Jeff Velten
Linscott's Directory .
GFP and FITC eric anderson
Transfecting CV1 cells Victor Levenson
48-Well microplates Alfred Zimmerlin
[Q]What is PMA converted to ? David L. Haviland, Ph.D.
Hybrizab libraries Daniel Durocher
GFP and FITC Bernard Murray
sequencing signal dies after 100 bases?! jfh
Yeast Multicopy Library Needed Toby Freedman
Which quantitative RT-PCR? Linda Perry
HOW to Eliminate PCR primer dimer Mary_Ann_Brow at TWT.COM
Tris base vs. Trizma Karl Voss
vector pMT22 anne borycki
Co-immunoprecipitation of nuclear proteins ege
PC restriction mapping software??? Brandon Harder
Deep Vent Pol (ssDNA) Fiberhead
Dissolve my pellet, please! Christoph.Lorra at urz.uni-heidelberg.de
Silver Staining Reveals Contaminating Bands in Sample Buffer Tom Buckley
Cloning problem Brendan Scott
Oligo problems The Great American Gene Company
Two-Hybrid System for E coli ? ajduclos at NEXUS.MICROIMM.MCGILL.CA
Transgenics Breandan Kennedy
Tris base vs. Trizma Dima Klenchin
Gradient Thermocyclers Kiley R. Prilliman
mAb Purification Difficulties Tom Thatcher
Expression of 2 proteins from different vectors Bret Sellman
Q: purifying lambda phage from extraneous DNA Greg Stuart
bright specks in EtBr stained gels - out, damn'd spot! Martin Goodson
48-Well microplates Keld Sorensen
sequencing signal dies after 100 bases?! Jan Hoolwerf
Q: purifying lambda phage from extraneous DNA David Winterbourne
Which Email reader? - Results Cornelius Krasel
Receptor Ligand Blot Pierre Hubert
Silver Staining Reveals Contaminating Bands in Sample B David C. Logan
Deep Vent Pol (ssDNA) Nick Jacobsen
Transfection of EBV-transformed B cell lines Kevin A. Mulcahy
High G/C PCR Dr. Duncan Clark
Q: purifying lambda phage from extraneous DNA Dr. Duncan Clark
Fragility of plasmids: size limit for shaking/vortexing? Frank O. Fackelmayer
Bordetella FHA James Good
Bordetella FHA James Good
Silver Staining Reveals Contaminating Bands in Sample Buffer Achim Recktenwald, PhD
Stratagene Site-directed mutagenesis zxiong at ag.arizona.edu
Restriction Enzymes in DNA Strider? Jim Woodgett
Co-immunoprecipitation of nuclear proteins jstrassw at OPAL.TUFTS.EDU
Bordetella FHA James Good
Q: purifying lambda phage from extraneous DNA John Dixon
Tris base vs. Trizma Alexander N. Kukushkin
Mini Mu bacteriophage albano renee
Q: purifying lambda phage from extraneous DNA Hiranya Roychowdhury
Q: purifying lambda phage from extraneous DNA Dr. Duncan Clark
electrophoresis at pH 8,0 Alexander N. Kukushkin
Silver Staining Reveals Contaminating Bands in Sample Buffer Huang Ke-xue
Calcium Phosphate Transient Transfection of NIH/3T3??? Alexander N. Kukushkin
painful gel loading???!!!!!! natldiagnos at mindspring.com
acid enzyme substrate?? ITABAJARA DA SILVA VAZ JUNIOR
Oligo problems Julie Roberts
superantigen genes.help.alternative method to Southern hybridisation sought Frans and Magda van Gils
Which Email reader? - Results Susan Jane Hogarth
Silver Staining Reveals Contaminating Bands in Sample Paul N Hengen
Q: purifying lambda phage from extraneous DNA Paul Mitsis
Pfu polymerase in PCR John Watson
Na-acetate in RNA Laura Woody
unsubscribe kwilson at DARWIN.UCSC.EDU
antigenic algorythms software/Fusion prot.s Ross Turbyfill
Na-acetate in RNA Dr. Peter Gegenheimer
Pfu polymerase in PCR Dr. Peter Gegenheimer
Oligo problems Jerry Kropp
superantigen genes.help.alternative method to Southern hybridisation sought Frans and Magda van Gils
Q: How to remove small size RNA from total RNA in bacteria? Jinling Xie
Laboratory Robotics Interest Group meeting Andy Zaayenga
superantigen genes.help.alternative method to Southern hybridisation sought Frans and Magda van Gils
Silver Staining Reveals Contaminating Bands in Sample David C. Logan
Q: How to remove small size RNA from total RNA in bacteria? Chi-kuang Wen
Stratagene Site-directed mutagenesis Greg
Stratagene QuikChange mutagenesis? Greg
Qustion on Ammonium sulfate and other precipitation methods Dima Klenchin
FOA problems-Help John Stebbins
LA PCR smuck Wayne M. Barnes
painful gel loading???!!!!!! Mike Gruidl
Q: purifying lambda phage from extraneous DNA Karl Fischer
help-stable horse blood agar-recipes Michael Heuzenroeder
48-Well microplates RManns7
Pins microplates cover RManns7
Mark.Ebdon@lifesciences.com Mark Ebdon
Cleaning up Transcriptions Alexander N. Kukushkin
Pfu polymerase in PCR Dr. Duncan Clark
DNA recovery by phenol/chloroform extraction or GeneClean Wilson
Enhancement of HRP substrate systems?? Joep
Stripping Northerns Martin Cann
Which Email reader? Juha Saharinen
Transfection of EBV-transformed B cell lines John Ladasky
Error rate in Taq pol. 1:270? derk at hubrecht.nl
Streptavidin and Biotin interactions Diane Webster
Transfection of EBV-transformed B cell lines Kiley R. Prilliman
Transfection of EBV-transformed B cell lines Dima Klenchin
T4 RNA Ligase Thomas J. Albert
Error rate in Taq pol. 1:270? Dr. Duncan Clark
Stratagene QuikChange mutagenesis? Troels Wind
E.Coli Plasmid<--->Chromosome Insertion/Recovery? Brad Nicholson
PCR/cloning of bacterial communities Bill Burnett
Stripping Northerns Klaus Salger
Aliquoting Antibodies for freezing - HELP! Pete
Antibody against GFP? David C. Logan
RNA mobility shift assay. Morten Andreas Geday
painful gel loading???!!!!!! Mark S. Rose
Seperation of NAD and nicotinamide by HPLC Heiko Koch
Error rate in Taq pol. 1:270? ICSC
Error rate in Taq pol. 1:270? Tom Chappell
E.Coli Plasmid<--->Chromosome Insertion/Recovery? Chris Boyd
Error rate in Taq pol. 1:270? Frank O. Fackelmayer
DNA recovery by phenol/chloroform extraction or GeneClean Frank O. Fackelmayer
ssDNA (M13) extract. with silica? Pascal Mertens
Cleaning up Transcriptions brett
Na-acetate in RNA David F. Spencer
fun with footprinting Heidi Moss
Pfu polymerase in PCR Frank O. Fackelmayer
Pins microplates cover martin LEACH
A solution for DNA or RNA contamination Dnacleaner
The solution for contamination Dnacleaner
Problems of contaminations? Think DNACleaner... Dnacleaner
PCR, NASBA, bDNA. Contaminations disturb your works? Dnacleaner
Simple, economic, DNAcleaner: a solution for contaminations Dnacleaner
You use frequently PCR or other technics...This is for you!!! Dnacleaner
DNAcleaner, french laboratories already use it against contaminations. Dnacleaner
Na-acetate in RNA Chi-kuang Wen
mean stacking temp. algorithm Les Hoffman
RNA extraction from small samples... Hiranya Roychowdhury
painful gel loading???!!!!!! martin LEACH
Where to buy E. coli NP66 for in vivo phagemid release Christian P. Sommerhoff
Pierce ECL and BioRad's phosphoimager system? Robert W. St. G. Fisher, IV
DNA recovery by phenol/chloroform extraction or GeneClean Sai
Two-Hybrid System for E coli ? Toumy Guettouche
DNA-free RNA Michelle Gleeson
Streptavidin and Biotin interactions Toumy Guettouche
adult-specific transgene promoter; dimattia@julian.uwo.ca Gabriel E. DiMattia
Chromosomal DNA carry-over in plasmid mini-preps Michael Mangan
Q: inducible promoters for plants Paul A Bucciaglia
pCR-Blunt vs pCR-Script bernielu at ERE.UMontreal.CA
Fusarium & NORTHERN BLOTS Cristina Placinta
[Q] : insect hemocyte culture Min-Ho Lee
Oligos: Who is cheapest? cynthia stryker
DNAcleaner, french laboratories already use it against contaminations. sunfish
sticky surfaces (fwd) Pradeep M Joshi
Chromosomal DNA carry-over in plasmid mini-preps martin LEACH
Using a PC as an assay counter-timer... Richard T. Timmer
SEARLE/MONSANTO JOINS ELECTRONIC NOTEBOOKS CONSORTIUM TO DEVELOP SYSTEMS TO PROTECT AND MANAGE INTELLECTUAL PROPERTY RECORDS FOR R&D Rich Lysakowski, Ph.D.
SEARLE/MONSANTO JOINS ELECTRONIC NOTEBOOKS CONSORTIUM TO DEVELOP SYSTEMS TO PROTECT AND MANAGE INTELLECTUAL PROPERTY RECORDS FOR R&D Rich Lysakowski, Ph.D.
sticky surfaces (fwd) Karl Fischer
ZAP systems F Dong
shrimp allergen Huangxu at space.ihw.co.cn
Stratagene QuikChange mutagenesis? Keith Rand
SEARLE/MONSANTO JOINS ELECTRONIC NOTEBOOKS CONSORTIUM Lysakowski at aol.com
SEARLE/MONSANTO JOINS ELECTRONIC NOTEBOOKS CONSORTIUM Lysakowski at aol.com
Transfection of EBV-transformed B cell lines John Ladasky
ZAP systems Andy Phillips
Pfu polymerase in PCR Dr. Duncan Clark
Error rate in Taq pol. 1:270? Dr. Duncan Clark
Thermal Cyclers, Evaluation Mark Hogben
HELP: where to obtain rat small intestine cDNA library?? Guido Hooiveld
Error rate in Taq pol. 1:270? Dr. Duncan Clark
ssDNA (M13) extract. with silica? Alexander Kraev
DNA recovery by phenol/chloroform extraction or GeneClean Wilson
subscribe Henry A. Daum, III
DNA recovery by phenol/chloroform extraction or GeneClean Wilson
subscribe Henry A. Daum, III
Error rate in Taq pol. 1:270? Martin Offterdinger
Error rate in Taq pol. 1:270? Dr. Duncan Clark
Oligos: Who is cheapest? Dr. Michael T. MacDonell
Oligos: Who is cheapest? Greg
Need gp160 clones for HIV IIIB, MN, BRU luscher at immune.med.utoronto.ca
Oligos: Who is cheapest? J. Pat Martinez
Using a PC as an assay counter-timer... Paul N Hengen
Problems with One Shot INValphaF' from Invitrogen sunfish
How many exonucleases have been discovered? Alexander N. Kukushkin
Error rate in Taq pol. 1:270? Paul N Hengen
LA PCR smuck Paul N Hengen
How many exonucleases have been discovered? Ludvig Mortberg
Stratagene QuikChange mutagenesis? Huang Ke-xue
Enhancement of HRP substrate systems?? Per Mygind
Using a PC as an assay counter-timer... Susan Jane Hogarth
Sequence of pT7-5 Philip Heacock
pCYPAC2 jcorral at gm.iro.es
Problems with One Shot INValphaF' from Invitrogen Martin Cann
Screening for Amp(s) bacteria. Rafael Maldonado
pGL3 / pGL2 dilemma: anybody working with these ? Alt Zantema
Room Sharing at 41st Biophysical Meeting Newswatcher
Cloning problem John Watson
Probing of an expression library with oligopeptides. Thomas
what kind of film? Michael Fitch
PCR-Select cDNA Subtraction Kit Victor Han
DD-RTPCR Victor Han
Inosine in degenerate primers Dr. Douglas A. Johnson
mean stacking temp. algorithm Jerry Kropp
Effect of DMSO on dsDNA? Jerry Kropp
Screening for Amp(s) bacteria. Paul N Hengen
anti-vinculin antibody u.381 at inserm.u-strasbg.fr
DNA recovery by phenol/chloroform extraction or GeneClean David Hackos
how to identify a gene on human chromsome 11 K-John Cheung
Problems with synthetic gene synthesis Gijs van Rooijen
Screening for Amp(s) bacteria. Susan Jane Hogarth
Cryostat sectioning... Jen Robertson
Streptavidin and Biotin interactions Toumy Guettouche
DNASpammer John Ladasky
Two-Hybrid System for E coli ? Toumy Guettouche
Problems with synthetic gene synthesis The Great American Gene Company
Anyone have a source for bovine cTnT? Jil Tardiff
Error rate in Taq pol. 1:270? Bryan L. Ford
Hirt Extractions Lawrence Mayer
(none) Hung-yiu Ho
DNA-free RNA Martin Offterdinger
(none) Hung-yiu Ho
Are these primer dimers? Frank O. Fackelmayer
Using a PC as an assay counter-timer... Frank O. Fackelmayer
Error rate in Taq pol. 1:270? Dr. Duncan Clark
The solution for contamination K.James
test... Gary Kucera
Inosine in degenerate primers Fraser Dr N J
Reference needed Dr Banwari Lal
Error rate in Taq pol. 1:270? Tom Chappell
Molecular clone of dehydroepiandrosterone Mary P. Remington
How to purify periplasmic fraction? Hayssam Zakaria
Using a PC as an assay counter-timer... Richard T. Timmer
DD-RTPCR Lou Alarcon
Collaboration offered: non-LTR retrotransposones study Alexander Blinov
Operon primer sequences Anita Burger
Error rate in Taq pol. 1:270? Richard P Grant
"Oligonucleotide purification method??" Balwi
pAb against GFP / EGFP Gunther Rezniczek
Checking labelling oligo Balwi
nylon mesh for hybridizations Marc Cortese
what kind of film? Alexander Kraev
Reference needed Elise Palmer
plasmid DNA needed (50-100 mg) Paul N Hengen
nylon mesh for hybridizations Derek Parsonage
PCR and poly a sequences. - wai1.doc [1/2] wlkl1j at clinmed.gla.ac.uk
2mL Concave Gradients??? Lou Cantolupo
PCR and poly A sequences, Wai Kwong Lee
beads for cell culture xxx
Immunoblotting on colony lifts Stephane Pasteau
Plant m-gfp5-ER (Haseloff) Mark Cock
Smearing of PCR product XIAO-MIN SU
I need graphics of the number of IgG (y) in function of time (x) ballet at enib.fr
Pfu polymerase in PCR Victor Levenson
beads for cell culture Jess H. Leber
RNA Splicing Software Jose Gonzales
Oligos: Who is cheapest? Victor Levenson
Limited digestion Daniel Auerbach
Screening for Amp(s) bacteria. Bernard Murray
Problems with One Shot INValphaF' from Invitrogen sunfish
plasmid DNA needed (50-100 mg) martin LEACH
Are these primer dimers? Hopkins, Charlotte
Spectrophotometers Karl A. Wilson
Inosine in degenerate primers The Great American Gene Company
Oligos: Who is cheapest? The Great American Gene Company
Molecular biology software suggestions Josh Goldstein
GFP expressing cell lines Jean-Philippe Hugnot
High G/C PCR Josh Goldstein
Pierce ECL and BioRad's phosphoimager system? Ann L. Williams
Pfu polymerase in PCR Colin Dolphin
2mL Concave Gradients??? David Micklem
PCR Techniques Workshop Mr. Ken Carter
rDNA Techniques Workshop Mr. Ken Carter
Quantitative RT-PCR J Kennie
nylon mesh for hybridizations Michelle Gleeson
FITC-Guard source? Richard Vernell
Pfu polymerase in PCR test
Application notes on respirometery of living organisms JanCzek
epitope tagging Toai T. Nguyen
nylon mesh for hybridizations EHeilweil
DNA analysis software WILL H SCOVILL
test Richard Daines
Screening for Amp(s) bacteria. Soeren G. Gatermann
Streptavidin and Biotin interactions John Richard Seavitt
CapFinder PCR cDNA library construction kit swathi padmanabhan
Disposal of Hoechst waste J. G. Gibbings
plasmid DNA needed (50-100 mg) Vincent Mulholland
Operon primer sequences Dr Alok Adholaya
GFP/time-lapse Neil Hotchin
in search of "Stains-All" staining protocol doering_t at mpimg-berlin-dahlem.mpg.de
Amplification from a single algal colony, any thoughts? Airlie Sattler
Need gp160 clones for HIV IIIB, MN, BRU Mark Luscher
Looking for ColV plasmid Steven Goldberg
Application notes on respirometery of living organisms JanCzek
Smearing of PCR product xudong huang
XGAL & IPTG -- how low can you go? Ann L. Williams
Smearing of PCR product Ann L. Williams
Are these primer dimers? Hopkins, Charlotte
Quick transformation protocol wanted Charles Brockhouse
"Oligonucleotide purification method??" Youhua Xie
stability of GFP in E.coli? Wolfgang Schechinger
XGAL & IPTG -- how low can you go? martin LEACH
Q: How to remove small size RNA from total RNA in Bacteria? Jinling Xie
Mechanism of EMS Action? Namjin Chung
How to purify periplasmic fraction? Troels Wind
Request for pUC7 Miss S.L. Stowell
beads for cell culture Troels Wind
DNA recovery by phenol/chloroform extraction or GeneClean Steven Goldberg
Non-specific binding of avidin woes. CLARKEY
Quick transformation protocol wanted Troels Wind
Smearing of PCR product Landry Pierre-Alexandre
Transposon mutagenesis john.sokatch at CCLINK.NET.UOKHSC.EDU
A freezer inventory software? vcore
XGAL & IPTG -- how low can you go? David L. Haviland, Ph.D.
DNA-free RNA orlando dominguez
Chromosomal DNA carry-over in plasmid mini-preps DSF
HELP:PAGE Probes Steve Scherer
sequence data anomaly-PCR clone jmorrey
nylon mesh for hybridizations martin LEACH
optimizing RT-PCR? Stefano casalotti
XGAL & IPTG -- how low can you go? martin LEACH
Q: How to remove small size RNA from total RNA in Bacteria? martin LEACH
Quick transformation protocol wanted Jeremy Marcus
Screening for Amp(s) bacteria. Szatmari George
High G/C PCR Nick Jacobsen
pCYPAC2 Nick Jacobsen
Mechanism of EMS Action? martin LEACH
Smearing of PCR product Nick Jacobsen
High G/C PCR Jerry Kropp
in search of "Stains-All" staining protocol Hiranya Roychowdhury
what kind of film? Cornelius Krasel
"Oligonucleotide purification method??" Cornelius Krasel
XGAL & IPTG -- how low can you go? David L. Haviland, Ph.D.
Quick transformation protocol wanted A.P.N. de Boer
Lamda phage - RIP? David L. Haviland, Ph.D.
beads for cell culture Jurgen Seppen
DNA analysis software Matthew L. Brown, Ph.D
3'RACE PCR czlq at musica.mcgill.ca
Inosine in degenerate primers John Ladasky
GCG Gurcharan Pahwa
Transfection Steve Cohen
Human cDNA libraries for two-hybrid system Sanjeeva Reddy
LPS-free DNA Thomas Adam
nylon mesh for hybridizations Marilyn Bakker
Lamda phage - RIP? martin LEACH
Quick transformation protocol wanted Colin Rasmussen
PCR and poly A sequences, Frank Beraud
Smearing of PCR product Frank Beraud
availability of cell lines with t(11;14) translocation Alex Dobrovic
Low infection of WEHI231 cell line with eco/ampho retrovirus tim
(none) cjvs at PUBLIC.CC.JL.CN
Wheat germ agglutinin staining. A.D. Delduca
Transfection brett
Test..Just Ignore Chang-Muk Lee
Circular permutation assay subtilis
Phi 29 DNA polymerase Gordon Munro
LPS-free DNA Sujeong Yun
protein phosphorylation wellman
e. coli expressing hsp90 David R. Bell
2D peptide mapping Roney Graf
Are there single letter codes for phosphoamino acids Peter Friedhoff
Non-specific binding of avidin woes. Keld Sorensen
Non-specific binding of avidin woes. Keld Sorensen
Pierce ECL and BioRad's phosphoimager system? Keld Sorensen
DNA analysis software Andrew Millar
Transposon mutagenesis Jeffrey David Bray
Transfection Lou Cantolupo
IL-4 assay on PBMC, help ! Jens Rainer Hansen
paraformalduhyde fixation and membrane integrity Andrew Doherty
A freezer inventory software? Susan Hubchak
paraformalduhyde fixation and membrane integrity Richard P Grant
inositol phosphate enzymatic hydrolysis Michel RIVIERE
rubidium chloride for making competent cells? Sai
yeast two hybrid R. Woodward
Real time PCR measurement Dr. J.P. Clewley
protein's pI czlq at musica.mcgill.ca
direct genomic sequencing hendrikb
mutation analysis hendrikb
3'RACE PCR Matthew L. Brown, Ph.D
Fusion Protein Expression Systems Steve
Are there single letter codes for phosphoamino acids Bernard Murray
Has anyone tried SNuPE ? Bernard Murray
protein's pI Randy Willis
Fusion Protein Expression Systems Randy Willis
FLAG epitope in yeast Jose Bonner
Disposal of Hoechst waste David F. Spencer
DNA recovery by phenol/chloroform extraction or GeneClean Bernard Murray
Sequencing???? Darren Mark Manley
Fusion Protein Expression Systems Ian A. York
electrophoresis units recommendations? peter
Are there single letter codes for phosphoamino acids Shaun D. Black
Inexpensive Oligo Purification? Namjin Chung
3' RACE PC Dr. N.A. Salazar
electrophoresis units recommendations? Michelle Gleeson
mean stacking temp. algorithm Michael Finney
how to identify a gene on human chromsome 11 Soonmyoung Paik, M.D.
RNA Splicing Software Soonmyoung Paik, M.D.
how much lambda vector do I need to use Ken Soderstrom
Quick transformation protocol wanted A.P.N. de Boer
luciferase Your Name
DNA analysis software Betsy L. Alberty
DNA recovery by phenol/chloroform extraction or GeneClean Betsy L. Alberty
Inexpensive Oligo Purification? The Great American Gene Company
epitope tagging Thomas R. Anderson
Calcium Phosphate Precipitation mennow at ruly46.medfac.leidenuniv.nl
paraformalduhyde fixation and membrane integrity Tsunehisa Namba
large amount of mRNA is needed hendrikb
Need gp160 clones for HIV IIIB, MN, BRU George M. Carter
Disposal of Hoechst waste Wolfgang Schechinger
A killer is needed Alex Borovkov
"Oligonucleotide purification method??" Paul Dunman
DNA recovery by phenol/chloroform extraction or GeneClean Paul Dunman
(none) Matthew David Petroski
My biology site (fwd) Sudeesha Kunjibettu, by way of C. S. Prakash
Fusion Protein Expression Systems Huang Ke-xue
How can I assay translational rate ?? u8251012 at cc.kmc.edu.tw
translational control ?? Yang Y.L.
translational control ?? Karl Fischer
e.mail subscription Rubina Shafi
AFLP plant mapping kit Jimmy
Quick transformation protocol wanted Eyck Poetter
Transposon mutagenesis Dr Banwari Lal
Soluene-350 composition/source? Mark
IMPACT Victor Han
cDNA Library Patrick HJ Falckh
Peptide nucleic acids (PNA) Ludla
Calcium Phosphate Precipitation Andrew Doherty
rubidium chloride for making competent cells? Nick Jacobsen
XGAL & IPTG -- how low can you go? Chris Boyd
Pfu polymerase in PCR Dr. Duncan Clark
bright specks in EtBr stained gels - out, damn'd spot! natldiagnos at mindspring.com
pEE14 Bjšrn Olde
myelin Sandra Supraha
myelin Sandra Supraha
Translational control Mr. G. Morley
Help! PCR linearity Joanne Rogers
Help! PCR linearity Joanne Rogers
Ecdysone Inducible System Chris Williams
Invitation to BioMOO Gustavo Glusman
Pfu polymerase in PCR Tom Duncan
Using a PC as an assay counter-timer... R. John Lye
Pfu polymerase in PCR Dr. Duncan Clark
Washing slides for in situs GILLIAN NI BHARLO BOTANY PG
HELP:PAGE Probes Wolfgang Schechinger
Calcium Phosphate Precipitation brett
Kozak concensus sequence jovermeyer at shrsys.hslc.org
PCR detection of RETROVIRUSES Raymond Henderson
2D peptide mapping Darren Tyson
protein's pI Darren Tyson
I need graphics of the number of IgG (y) in function of time (x) martin LEACH
Transfection martin LEACH
direct genomic sequencing martin LEACH
luciferase martin LEACH
Disposal of Hoechst waste Rolf Marteijn
Antisense transcripts Zhiyong Zhao
(none) vduke at ANCON.UP.AC.PA
(none) vduke at ANCON.UP.AC.PA
yeast two hybrid Martin Gossen
Stop codon read-through? Harris Laboratory
Histone H1 size? Rizwan Haq
E. coli (Bl21DE3) lyses automatically Huang Ke-xue
Gene organization Sigurd Ørstavik
epitope mapping kit Jimmy Loh
Kozak concensus sequence brett
acid phenol extractions sunfish
E. coli (Bl21DE3) lyses automatically Greg
293 cells in serum-free medium? Lloyd Graham
Kozak concensus sequence Gary Kucera
Knockout mice advice Karen
Knockout mice advice Karen
WANTED: Src construct Mr. G. Morley
RNA Ligase Matt Thomas
E. coli (Bl21DE3) lyses automatically Dr. Duncan Clark
DNA recovery by phenol/chloroform extraction or GeneClean Chris Boyd
Kozak concensus sequence Deborah A. Samac
address Jenny Allsop
Help suppliers of biotinylated magnetic beads a.molloy
AATAAA signal czlq at musica.mcgill.ca
acid phenol extractions brett
IMPORTANT: Eukaryotic vector sequences! Ole Gjoerup
single tube nested pcr gzk at ollamh.ucd.ie
Transient transfection of Cos-7 (electroporation v DEAE dextran) Kevin A. Mulcahy
Request for integrin plasmids or antibodies. PLEASE. CLARKEY
E. coli (Bl21DE3) lyses automatically Justinian
fluorescent sequencing of cosmids David D Morgan
anti-prion protein Julie Roberts
Protein Expression Klaus Lun
Protein Expression Klaus Lun
E. coli (Bl21DE3) lyses automatically Albert Crescenzo
Pfu polymerase in PCR Martin Hughes
E. coli (Bl21DE3) lyses automatically Randy Willis
Protein Expression Randy Willis
bright specks in EtBr stained gels - out, damn'd spot! Klaus Salger
Transposon mutagenesis Paul N Hengen
AATAAA signal martin LEACH
Help suppliers of biotinylated magnetic beads martin LEACH
Quick transformation protocol wanted Paul N Hengen
translational control ?? Bernard Murray
Protein Expression Dima Klenchin
Screening for Amp(s) bacteria. Paul N Hengen
Stop codon read-through? Rafael Maldonado
How much thrombin for GST fusions ? Tom Haenel
multiplying bands in gel purification John G. Luz
pH of water; dissolving RNA Hildur V. Colot
DNA recovery by phenol/chloroform extraction or GeneClean William Coetzee
epitope tagging background in plants chen0183 at maroon.tc.umn.edu
Monoclonal vs polyclonal antibody Levon Fendekian
Transient transfection of Cos-7 (electroporation v DEAE dextran) Rick Nicholson
Low RNA yield after Qiaquick column purification. Zhongtang Yu
Pfu polymerase in PCR John Ladasky
Anyone Using Competimer (TM) Technology for RT-PCR? Richard Rohan
expression vector problem wliu at receptor.pharm.uwa.edu.au
Stop codon read-through? C. J. Fields
Stop codon read-through? C. J. Fields
pH of water; dissolving RNA Alex Dobrovic
acid phenol extractions Ben Haskell
Primer design strategy? hcorbett at garnet.berkeley.edu
293 cells in serum-free medium? Jarkko Kortesmaa
Pfu polymerase in PCR Tom Chappell
Checking labelling oligo Marcus Rattray
removal of PEG from DNA disolved in TE? Venkat Pisupati
How much thrombin for GST fusions ? Curt Ashendel
pH of water; dissolving RNA Curt Ashendel
nonspecific primer extension products Barr Lab
Strange contaminant in cell culture Obaid Y. Khan
learning to use newsgroup boukhb01 at mcrcr6.med.nyu.edu
removal of PEG from DNA disolved in TE? Richard P Grant
Q:Measurement of protein's surface hydrophobicity? Achim Recktenwald, PhD
Cosmid cloning recommendations? Volker Knoop
refolding from inclusion bodies juhi juneja
Scanalytics RFLPscan David Ley
Strange contaminant in cell culture Richard P Grant
Pfu polymerase in PCR Dr. Duncan Clark
dam-/dcm- bugs and mutations?? Dr. Duncan Clark
Strange contaminant in cell culture brett
Stop codon read-through? Dr. Duncan Clark
removal of PEG from DNA disolved in TE? Dr. Duncan Clark
Westerns and estrogen receptors Aideen O Doherty
SR-B1 (scanvenger receptor B1) required Benoit Gauthier
refolding from inclusion bodies Gouzel Karimova
multiplying bands in gel purification William Coetzee
DNA-quantitation Joern Schwede
refolding from inclusion bodies bipin k. dalmia
no-BglI plasmid Peter Cheung
Smoking and smoke interactions with medications MHPERTG at iris.rfmh.org
3'RACE PCR John Watson
3-Amino triazole conc.? Martin Gossen
Free webzine for BioMedNet members Huang Ke-xue
pH of water; dissolving RNA Achim Recktenwald, PhD
Conductance Standards Leon Scott
Strange contaminant in cell culture Dima Klenchin
DNA sequence comparision wjc at acsu.buffalo.edu
DNA sequence comparision martin LEACH
Deglycosylation of proteins: questions Dale Karlson
GFP S65T vectors Hildur V. Colot
E. coli (Bl21DE3) lyses automatically Huang Ke-xue
?-easiest clean-up for sequencing Tom Duda
epitope mapping kit Ross Turbyfill
Monoclonal vs polyclonal antibody Ross Turbyfill
multiplying bands in gel purification Jill Kellogg
refolding from inclusion bodies Randy Willis
Pfu polymerase in PCR Tom Duncan
Summary: Screening for Amp(s) bacteria. Rafael Maldonado
DNA quantitation Dick Haugland
Q:Measurement of protein's surface hydrophobicity? "Uncle Al" Schwartz
Stable Thp1 transfections Guriq Basi
Transient transfection of Cos-7 (electroporation v DEAE dextran) George
Q: Source of terminating ribonucleotides Igor Sidorenkov
bright specks in EtBr stained gels - out, damn'd spot! Rich Showalter
5'-psoralen-labelled primers Karel Wernars
Custom DNA-Sequencing in Europe FABIAN MOEBIUS
hot PCR Rob Jordan
northern blotting a.mccormick
hyperfilm-MP/hyperfilm-ECL Federici
A review on bacterial promoter consensus sequence Jean-Francois Mayaux
SDF-1 antibodies to get or buy Morgane BOMSEL
SDF-1 antibodies Morgane BOMSEL
Pfu polymerase in PCR Dr. Duncan Clark
Help suppliers of biotinylated magnetic beads Nick Jacobsen
S.aureus primers laban
Long PCR & Joining 5' of sequence to the rest Harry Witchel
multiplying bands in gel purification vilimf01 at mcrcr6.med.nyu.edu
Reamplification pcr products after Ag-staining Michael Vork
How can I assay translational rate ?? Dr E. Buxbaum
multiplying bands in gel purification T.S. Grewal
biometra vs perkin elmer thermal cycler Di James
Available statistical programs Mark Lamkin
DNA sequence comparision Ashok Aiyar
Pepsin: IU to FIP unit ???? H Chang
protein solubility problems Malcolm M. Campbell
double labeling with BrDU Danny Altschuler
Wanted: Tie2 expressing cell line. XXX
YAC libraries J. G. Gibbings
Wanted: Tie2 expressing cell line Medvinsky
Problems with synthetic gene synthesis (Summary of replies) Gijs van Rooijen
dam-/dcm- bugs and mutations?? Victor Levenson
northern blotting Bernard Murray
Sequencing and sharkteeth Jørgen Stenvik
Protein Extraction Buffers...What's a good homogenization buffer for westerns? Rob Wesley
refolding from inclusion bodies Bob Steinberg
differentiating between genomic & plasmid DNA E.Hillery
double labeling with BrDU Jorg Kirberg
Conductance Standards Frank R. Gorga
hot PCR Caroline Szymeczek-Seay
Desire recommendation re 2-D gel apparatus Jerry Kropp
multiplying bands in gel purification Jurgen Seppen
Reamplification pcr products after Ag-staining John Watson
Sequencing and sharkteeth Michelle Gleeson
Sequencing and sharkteeth Randy Willis
nonspecific primer extension products martin LEACH
nonspecific primer extension products martin LEACH
trypsin magnetic beads Mick Foley
DNA sequence comparision Matthew L. Brown, Ph.D
Jurkatt cell induction rayj
Immunohistochemistry help melvnr at hg.uleth.ca
beads for cell culture Dlh4image
A review on bacterial promoter consensus sequence Gerd Nilsen
Protein Extraction Buffers...What's a good homogenization buffer for westerns? Malcolm M. Campbell
dam-/dcm- bugs and mutations?? Dr. Duncan Clark
hyperfilm Federici
dam-/dcm- bugs and mutations?? Chris Boyd
Strange contaminant in cell culture Tom Vink
Looking for ColV plasmid Chris Boyd
SSC conc. in prehyb. Denni Schnapp
hot PCR Nick Jacobsen
N-terminal sequence Emile Schiltz
Tris base vs. Trizma Denni Schnapp
dam-/dcm- bugs and mutations?? Dr. Duncan Clark
screening of cDNA library: custom services Myriam Onno
Molecular modelling capabilities Dr. Duncan Clark
differentiating between genomic & plasmid DNA Hiranya Roychowdhury
Help: Bacterial Genomic DNA extraction and library construction Joel Bader
Geneskipper vs DNASIS FABIAN MOEBIUS
differentiating between genomic & plasmid DNA Alexander Kraev
Q:Measurement of protein's surface hydrophobicity? lgbell at liverpool.ac.uk
Primer design Yang Y.L.
Taq polymerase inhibition reference Michael Douglas
Proteinase K and detergent Jill Kellogg
SSC conc. in prehyb. Chi-kuang Wen
test Ray Boykiw
refolding from inclusion bodies Sourav Ghosh
subscription to a newsgroup Francis ROBERT
pH of water; dissolving RNA Chi-kuang Wen
anti-prion protein martin LEACH
Transposon mutagenesis Rafael Maldonado
Pfu polymerase in PCR Tom Duncan
Na+/K+ ATPase assay J Hance
Q: How to remove small size RNA from total RNA in bacteria? Dr. Peter Gegenheimer
skatron? Nancy Linford
Deglycosylation of proteins: questions hadi al-hasani
RNase Protection Assay mennow at ruly46.medfac.leidenuniv.nl
Pfu polymerase in PCR Dr. Duncan Clark
INDEX MEDICUS address Jenny Williams
PYMS Diagram Jenny Williams
Transposon mutagenesis Brad Nicholson
quick question Rani Geha
error rate reverse transcriptases F.Schaap at FYS.UNIMAAS.NL
oligo biotinylation? Yi Zhang (BIO)
Molecular modelling capabilities George A. Heavner
beads for cell culture Robertoalf
beads for cell culture Zhongtang Yu
quick question Karl Fischer
quick question Hiranya Roychowdhury
what kind of film? Phillip Robinson
best total RNA and mRNA isolation kits Karim Hyder
transfection/transformation E.Hillery
LB contains glucose? hbkg at hitel.kol.co.kr
Expression of bcl-2 homologue bad Reinhard Hoffmann
Stability of Doxycycline? Stephan Geley
Q:measurement of protein's surface hydrophobicity Kurt L Brillhart
Na+/K+ ATPase assay lincoln at indigo.ie
jwc-nondenaturing geo of protein Loesch-Fries Lab
Limited digestion Thon de Boer
phosphatase detection in yeast ... Daniel O. Espinoza
Help!Mouse fibroblast cell line dying! Tori D. Williams
The Antibody Resource Page Kevin Shreder
Fusion Protein Expression Systems LouPass
RNase Protection Assay Antonin Tutter
Sequencing and sharkteeth martin LEACH
quick question martin LEACH
quick question Bernard Murray
best total RNA and mRNA isolation kits Timo Hiltunen
Hirt extractions Graeme J. Dougherty
Long PCR & Joining 5' of sequence to the rest John Ladasky
Desire recommendation re 2-D gel apparatus John Ladasky
help with pcr for dna from sediments Guenter Jost
BIOSCI/bionet miniFAQ & Fundraiser BIOSCI Administrator
LB contains glucose? Cornelius Krasel
Immunoblotting on colony lifts Pascal Mertens
INDEX MEDICUS address Volker Knoop
quick question Ulrich Melcher
mRNA isolation kits for plant culture tissue nhwu at PUBLIC3.BTA.NET.CN
PCR czlq at musica.mcgill.ca
quick question Karl Fischer
Gancylovir Supplier David Hills
Primer design John Watson
best total RNA and mRNA isolation kits Karim Hyder
transfection/transformation Alan KAY
Adhesive temperature indicator / warning for reagants & buffers Charles_Lasitter
oligo biotinylation? Darren Tyson
address STEEVE VERONNEAU
Transient transfection of Cos-7 (electroporation v DEAE dextran) Steeve Veronneau
Antibody against T7 RNA Polymerase Alain Kohl
Acetamide in PCR reaction? Anton Scott Goustin
pLAFR1 cosmid restr. maps, anyone? weezer Mccheese
transfection/transformation Anton Scott Goustin
How can I assay translational rate ?? Anton Scott Goustin
Where to be published Michael Almeida
Q:Measurement of protein's surface hydrophobicity? Bill Van Antwerp
Na+/K+ ATPase assay J Hance
Cutting DNA from non-E.coli Eileen Hickey
Antisense oligonucleotides Sandra Pena De Ortiz
Reamplification pcr products after Ag-staining rebecca slayton
long PCR with dUTP Michael C. Gorry
Jurkatt cell induction Anton Scott Goustin
RNA from urine nik
constitutive gene in bacteria NG WAI LEUNG
quick question Kiley R. Prilliman
3-Amino triazole conc.? Andy
Seeking secretion vector to express MFalpha-fusion protein James Irving
screening of cDNA library: custom services --
Green Fluorescent Protein Purificaton Short Course Daniel Gonzalez
long PCR with dUTP Timo Hiltunen
long PCR with dUTP Dr. Duncan Clark
Custom DNA-Sequencing in Europe H.J. Sluiman
Q:Measurement of protein's surface hydrophobicity? Dr S. Robertson
WANTED: pTrc99 plasmids Ed Schwartz
Adhesive temperature indicator / warning for reagants & buff Wolfgang Schechinger
Help on bacterial analysis? l95mn at river.tay.ac.uk
Looking for cDNA sequence or accession number of IRS-2 Wolfgang Schechinger
dot blot with bacteria Stephane Pasteau
quick question John Watson
removal of PEG from DNA disolved in TE? vilimf01 at mcrcr6.med.nyu.edu
best total RNA and mRNA isolation kits Julie Roberts
ATP SARAH LOUISE RAWLINGS
rRNA contamination after RNA poli-A purification with oligo-dT CARLOS ALEXANDRE SANCHEZ FERREIRA - 218
?-easiest clean-up for sequencing vilimf01 at mcrcr6.med.nyu.edu
Cutting DNA from non-E.coli Michael Benedik
Kodak Biomax Vs Fuji RX frogs 'r' us
PVP/PVPP Hiranya Roychowdhury
Q: PCR cloning Jinling Xie
Primer design Michael Kertesz
test XiaoYin Zhong
blotting acrylamide gels Constable Julie Lynn
nonspecific primer extension products Rafael Maldonado
Gridded Membranes Rory O'Brien. Biotechnology.
meaning of acronym Regina Shaw
Help: cleaning sintered glass funnels Guy Oshiro
Sea Urchin and Manduca cDNA libraries Greg Beck
anti-prion protein sas96igg at rdg.ac.uk
blotting acrylamide gels Michelle Gleeson
Custom DNA-Sequencing in Europe Francois Coulier
RER+ mouse cell lines Bob Steinberg
Gridded Membranes Hiranya Roychowdhury
Problem with nonspecific binding DiverNLB
Mac-based commercial mapping program GEN282E at CCS1.cc.monash.edu.au
quick question martin LEACH
Where to be published martin LEACH
Looking for cDNA sequence or accession number of IRS-2 martin LEACH
Gridded Membranes martin LEACH
WANTED: Human Genomic (Cosmid) Library jxie at bi-pharm.com
Rodent (mouse or rat) microsat. primers wanted.... Dave Edwards
(none) Takeshi SAITO
Help: cleaning sintered glass funnels Bernard Murray
Protein-protein interaction of His-tag of pET14b? Jimmy S.H. Tsang
quick question Bernard Murray
**************Gancyclovir********************** David Hills
T7select phage display Liselotte Kahns
Help: cleaning sintered glass funnels Keld Sorensen
Disposable counting chamber Eike
Dissociating agents to prevent protein aggregation newera at plaza.snu.ac.kr
Looking for cDNA sequence or accession number of IRS-2 Peter Pichler
VHD strain request Stephane Pasteau
Looking for cDNA sequence or accession number of IRS-2 - whole text Peter Pichler
Questions about DNA recovery and cloning Wilson
monoclonal antibody for HA Krista Fehr
Question about genomic library construction Wilson
best total RNA and mRNA isolation kits Beisvåg Vidar
?-easiest clean-up for sequencing David L. Haviland, Ph.D.
Horseradish Peroxidase assay Tom Parish/Cambridge/Biogen
differentiating between genomic & plasmid DNA Marieke R. Koedood Zhao
multiplying bands in gel purification QIAGEN
**************Gancyclovir********************** Toumy Guettouche
Horseradish Peroxidase assay Sanjay Krishnaswamy
TRAP assay xin wei
Northern blot RNA degradation Jos
Questions about DNA recovery and cloning Alexander N. Kukushkin
tempest on a toothpick Tom Cooper
Question about genomic library construction Alexander N. Kukushkin
Breakthroughs in SPM Mark Flowers
blotting acrylamide gels John Watson
Clontech GFP Alex Harvey
Q: PCR cloning Jan Michel
Source of bismuth subcitrate in N. America? REW
TRAP ASSAY Xin Wei
WANTED: Vector to express MF-alpha fusion protein James Irving
Single Copy Plasmids Brett Beitzel
microsatellite loci amplification kfeldh1 at ICARUS.CC.UIC.EDU
PCR contamination Stsarev
yeast 2 hybrid screen on heart cDNA library Malathi Hari
does anyone have a spac-promoter vector Neil Mattatall
How to use BLAST Zhencai Wu
Making Nickel resin?? Stephen W Doughty
**************Gancyclovir********************** Jurgen Seppen
Apparati for Northern Blotting DiAnna L. Hynds
Questions about DNA recovery and cloning Hiranya Roychowdhury
PCR contamination Dr. J.P. Clewley
Primary embryo fibroblasts transfection Rizwan Haq
Agrobacterium M Allen
Agrobacterium M Allen
Molecular Modellers - Wanted Michael Parkinson
Question about genomic library construction Wilson
Question about genomic library construction Huang Ke-xue
monoclonal antibody for HA Thomas R. Anderson
ADVANE IMMUNOFLUORESCENE COURSE Paolo Castano
**************Gancyclovir********************** Przemko Tylzanowski
anti-prion protein Andy Hill
Question about genomic library construction K.James
T7 or SP6 RNA Pol promoter sequence Gordon Munro
?-easiest clean-up for sequencing Alexander Kraev
Test Post Ed Wang
Mycoplasm test by PCR S Von
Questions about DNA recovery and cloning Steven Goldberg
Making Nickel resin?? Curt Ashendel
does anyone have a spac-promoter vector H. Yim
Single Copy Plasmids Karl Fischer
Limited digestion Marieke R. Koedood Zhao
RNase Protection Assay Marieke R. Koedood Zhao
hydroxymethyl dCTP Les Hoffman
Making Nickel resin?? wegloehner at mpimg-berlin-dahlem.mpg.de
Question about genomic library construction Steven Goldberg
Cambridge Healthtech Institute’s MOLECULAR EVOLUTION Cambridge Healthtech Institute
Transformation protocol for Bacillus needed Arul Jayaraman
A BiaCore-like system Randy Willis
microsatellite loci amplification Charles Brockhouse
(none) Benoit Gauthier
guanylyl cyclase antibody m hayden
Reamplification pcr products after Ag-staining C.K. Chen
2-D software for Mac? Jerry Kropp
RNA secondary structure A. Kumar
Random peptide library Richard Vernell
Hot plasmid + Cold Vector = Insert-specific probe???? Michael J. Prigge
blotting acrylamide gels Jerry Kropp
Northern blot RNA degradation Klaus Salger
integrin antibody ANNE GEORGE
Best commercial Factor Xa? alan friedman
Hirt procedure pl. Patil J G
Help Guy McDonnell
Adhere cells onto slides for immunostaining Susan Hubchak
Adhere cells onto slides for immunostaining Zheng Huanquan
degenerate PCR Dr Koen A.L. De Smet
Question about genomic library construction Wilson
transgenic bacteria Stephane Pasteau
Sequencing and sharkteeth Edwin Abeln
RNA secondary structure Tom Vink
Hot plasmid + Cold Vector = Insert-specific probe???? Alexander Kraev
degenerate PCR Tim Chipman
Biorad Quantum minipreps re ALF sequencers Oliver S. DePeyer
Ph.D. Assistantship Available Dr. Jeffrey Dean
northern blotting Sam Gray
best total RNA and mRNA isolation kits Sam Gray
Q: Clones from genomic libraries? Bart Nelissen
heart 2hybrid screen Malathi Hari
Methicillin suppliers? Derron Blakely
Dexamethasone-inducible pMAMneo vector Marit Anthonsen
How to separate vegf dimer into monomer? Ziping Yang
PCR Darren Mark Manley
differentiating between genomic & plasmid DNA J. Pat Martinez
?-easiest clean-up for sequencing Dr S.K. Coleman
gel doc/photo imaging system Hoyun_Lee at octrf.on.ca
Sectioning mouse eyes Help! Kevin Rattray
test Ray Boykiw
Effect of Phenol degradation prodcust on bacterial bioluminescence corbisip at vito.be
biotinylation of BBMV Edwin Alcantara
Check Shiwani Arora
Dexamethasone-inducible pMAMneo vector Bernard Murray
A little help here... The Kindly One

Last message date: Fri Feb 28 22:10:36 EST 1997
Archived on: Sun Mar 6 21:22:10 EST 2005

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