Methods February 1997 Archives by thread
Starting: Sat Feb 1 00:52:13 EST 1997
Ending: Fri Feb 28 22:10:36 EST 1997
Messages: 1105
- SYPRO Orange Quenching by 35-S
Dick Haugland
- PCR product into northern probe ??
u8251012 at cc.kmc.edu.tw
- pGL3 / pGL2 dilemma: anybody working with these ?
Dr. J. Miano
- Gene Finder
Dr. J. Miano
- SV40 poly(A) signal works bidirectionally?
Hiroyoshi Ohba
- digesting the ends of PCR products
Martin Hughes
- MMLV and rNTP's
Matt Thomas
- Oligo problems
Arthur Wohlwill
- Short seq search?
Chihara
- PCR product into northern probe ??
Youhua Xie
- Question: Cytoplasmic and nuclear fractionation of cells.
John Pawlowski
- cDNA product separation
Yang Y.L.
- RT-PCR question !
Yang Y.L.
- Hydropathy plot software wanted
Oliver S. DePeyer
- Need tips for sequencing through poly-c tail
Scott Hodgson
- HOW to Eliminate PCR primer dimer
u8251012 at cc.kmc.edu.tw
- (no subject)
Joan Boyce
- Monoclonal CAT
Joan Boyce
- Boehringer DIG system experiences
Karen Ferri
- HOW to Eliminate PCR primer dimer
Huang Ke-xue
- Protein Labeling? Casein Kinase?
Thomas J. Albert
- Image Analysis Shareware
Ted M.
- Image analysis help needed
SCROOD
- Emission wavelength of EtBr/DNA for a CCD camera filter
Dr. Duncan Clark
- Q: How doing partial RE digests?
Sanjay Shirke
- Vol of ligation mix to use in transformation
Keith Warner
- Thermotoga maritima DNA/cells
alan friedman
- Neomycin/Kanamycin Resistance
C. J. Fields
- Q: inducible promoters for plants
Dr. K. Eimert
- Which Email reader?
Graham Atherton
- Subtractive Probe
charles lawrie
- Phenol Protocol
Alexandre Noël
- RNA standards for quantitative PCR
Adrian Allan
- Selling a used Beckmann rotor
Roger C. Green
- pH dependence of DNA hydrolysis
Shuiliang Shi
- Q re: PCR from agarose plugs
Steven Sullivan
- PCR primer-dimer elimination
Robert B. Chadwick
- HPRT targeting vector
Constanze Bonifer
- Phenol Protocol
David L. Haviland, Ph.D.
- roller bottle culture
shanthi adimoolam
- TEST
HARIHARAN P.EASWARAN
- P.E. 9600 thermocyclers-the cooling fix...
Christopher G. Alpha
- Growth and transformation of XL1-Blue
Kevin A. Mulcahy
- DGGE Gel Drying...?
Dr Steve Griffiths Molecular Biology Group
- pH for RNA work
Dr Banwari Lal
- digesting the ends of PCR products
Steven Goldberg
- Transfecting CV1 cells
Lourdes Rodgers
- Pointer to FAQ lists
Paul N Hengen
- Membrane preps
Brenda
- Want to hook DNA/RNA to solid matrix
HARIHARAN P.EASWARAN
- cleaning pipettes
David Voduc
- Help with PCR adding sp6 sequence
william wu
- digesting the ends of PCR products
Ashok Aiyar
- Primer Design
Mirna Perez
- Subtracted cDNA Probe
Barbara
- DNA extract with CTAB
Brendan Scott
- Cloning problem
Brendan Scott
- Q re: PCR from agarose plugs
Bob Steinberg
- Number of Bacteria per plate?
TJ
- DNA-free RNA
Francois Hatey
- pH for RNA work
John Morlan
- Sigma Monoclonal Anti-polyHis
David Ackerley
- pH for RNA work
Bernard Murray
- DNA-based assay of apoptosis needed
Jerry Kropp
- Quntifying small amounts of DNA reliably
Natalie Bloch
- FISH protocols
Paul Lee
- FISH protocols
Paul Lee
- Primer Design
Natalie Bloch
- I can't get my multichannel pipette into my DNA engine.....:-(
Gordon Munro
- sequencing signal dies after 100 bases?!
Jan Hoolwerf
- Boehringer DIG experiences
Andrew Bettany
- Preparation of rifampicine
C E Pical
- Na-acetate in RNA
RUTH MCMAHON
- peptide hormone assays
Rory Morty
- I need more infor about versene
Richard P Grant
- Calculation of transformation efficiency
Kevin A. Mulcahy
- Transfecting B cells
Erik Kul| Hagen
- E.Coli Plasmid<--->Chromosome Insertion/Recovery?
Mark Blight
- I need more infor about versene
David L. Haviland, Ph.D.
- Q: How doing partial RE digests?
Mark Blight
- Calculation of transformation efficiency
David L. Haviland, Ph.D.
- Imidazole buffer has esterase activity?
Steven Goldberg
- Restriction Enzymes in DNA Strider?
Namjin Chung
- GST-fusion protein extraction
Philippe Pognonec
- XGAL & IPTG -- how low can you go?
Glen Shearer
- Molecular biology software suggestions
Debbie Moore
- Growth and transformation of XL1-Blue
Alexander N. Kukushkin
- Km calculation web site
Huang Ke-xue
- DNA-based assay of apoptosis needed
Jerry Kropp
- 220 RNA peak
Wendy Stewart
- Dissolve my pellet, please!
Josh Berke
- subcloning large fragments
Frederic G. Barr Lab
- Molecular biology software suggestions
Matthew L. Brown, Ph.D
- GFP and FITC
Danny Altschuler
- retroviral particle size?
Danny Altschuler
- subcloning large fragments
brett
- Calculation of transformation efficiency
brett
- contamination of glycine by other amino acids ???
Queen of Molecular Biology
- Restriction Enzymes in DNA Strider?
Harry Mangalam
- subcloning large fragments
David L. Haviland, Ph.D.
- subcloning large fragments
pmiguel at bilbo.bio.purdue.edu
- Measuring DNA Concentration: UV absorption shift?
Yvonne Thorstenson
- radioactive actinomycin D
William Sun
- How to perform a DNA Methyltransferase assay?
Francisco J. Alarcon
- retroviral particle size?
Dima Klenchin
- Need to find Supplier of Anti-Neo
Blair Jennings
- I need more infor about versene
Forget-me-not
- Article Search
Martin Beaulieu
- subcloning large fragments
Bernard Murray
- test - please ignore
Granny
- High G/C PCR
Dr Koen A.L. De Smet
- HDC antibody
Yoshitaka Konda
- (none)
Zhuang
- nucler extract
doering_t at mpimg-berlin-dahlem.mpg.de
- Heparin in shift assays
doering_t at mpimg-berlin-dahlem.mpg.de
- Efficient method for analysis of recombinants?
Greg Nattrass
- How much DNA can be "crowded" into a solution?
E.Hillery
- Fragility of plasmids: size limit for shaking/vortexing?
Kevin A. Mulcahy
- electrophoresis at pH 8,0
Peter
- Calculation of transformation efficiency
Alexander N. Kukushkin
- DNA curvature software
subtilis
- Revs vs. g's in centrifugation
Mr. G. Morley
- Helix pomatia cDNA
Ute Wittstock
- <<test>> ignore
Glen Moore
- Methods for in vitro mutagenesis
Kellie Ann Lugenbeel
- fos messenger size
W. Schechinger
- fos mRNA size
Wolfgang Schechinger
- How much DNA can be "crowded" into a solution?
Ludvig Mortberg
- Trcky vectors
Ed Schwartz
- Luciferase
Yuriko Suzuki
- Fragility of plasmids: size limit for shaking/vortexing?
Alexander Kraev
- TEST -- ignore
Glen Moore
- Revs vs. g's in centrifugation
Darren Natale
- mAb Purification Difficulties
Patricia Keely
- pH for RNA work
David L. Haviland, Ph.D.
- Tris base vs. Trizma
ege
- sources of hsp90?
Sai
- RNA from tiny amounts of tissue?
John Watson
- recombinant protein labelling with methionine
curmi at infobiogen.fr
- Calcium Phosphate Transient Transfection of NIH/3T3???
john storring
- E.coli for Electroporation
Helen McBride
- large amount of mRNA is needed
Rafael Maldonado
- RNA extraction from little tissue.
Hiranya Roychowdhury
- Pfu polymerase in PCR
Bob Steinberg
- digesting the ends of PCR products
AMICVS CONSTANTINI
- Crustacean ITS Phylogeny and PCR
Ari Kahn
- Co-immunoprecipitation of nuclear proteins
Richard Dahl
- Electroelution
Steven Sanyal
- GFP and FITC
Dick Haugland
- imidazole buffer has esterase activity
Dick Haugland
- RNA from tiny amounts of tissue?
Alex Dobrovic
- DNA SOFTWARE INFORMATION PLEASE
CLARKEY
- Deep Vent Pol (ssDNA)
Moez TORKI
- Probing of an expression library with oligopeptides.
CLARKEY
- Pins microplates cover
Grognet Pierre
- Qustion on Ammonium sulfate and other precipitation methods
Cornelius Krasel
- Which Email reader? - Results
Graham Atherton
- quantifying small amounts of DNA
Mr. G. Morley
- [Q]What is PMA converted to ?
Hisanori Yamamoto
- antigenic algorythms software/Fusion prot.s
darren cikaluk
- Q: inducible promoters for plants
Curt Ashendel
- paranitrophenyl acetate
Bernard Dumas
- Q: inducible promoters for plants
Hiranya Roychowdhury
- DNA Sequencing
DSF
- bright specks in EtBr stained gels - out, damn'd spot!
Sam Michaelson
- Probing of an expression library with oligopeptides.
Sai
- Q: inducible promoters... retraction!!
Hiranya Roychowdhury
- Linscott's Directory
.
- 48-Well microplates
Alfred Zimmerlin
- [Q]What is PMA converted to ?
David L. Haviland, Ph.D.
- Hybrizab libraries
Daniel Durocher
- Yeast Multicopy Library Needed
Toby Freedman
- Which quantitative RT-PCR?
Linda Perry
- HOW to Eliminate PCR primer dimer
Mary_Ann_Brow at TWT.COM
- vector pMT22
anne borycki
- PC restriction mapping software???
Brandon Harder
- Silver Staining Reveals Contaminating Bands in Sample Buffer
Tom Buckley
- Cloning problem
Brendan Scott
- Two-Hybrid System for E coli ?
ajduclos at NEXUS.MICROIMM.MCGILL.CA
- Transgenics
Breandan Kennedy
- Gradient Thermocyclers
Kiley R. Prilliman
- Expression of 2 proteins from different vectors
Bret Sellman
- Q: purifying lambda phage from extraneous DNA
Greg Stuart
- Receptor Ligand Blot
Pierre Hubert
- Silver Staining Reveals Contaminating Bands in Sample B
David C. Logan
- Transfection of EBV-transformed B cell lines
Kevin A. Mulcahy
- Bordetella FHA
James Good
- Bordetella FHA
James Good
- Stratagene Site-directed mutagenesis
zxiong at ag.arizona.edu
- Bordetella FHA
James Good
- Tris base vs. Trizma
Alexander N. Kukushkin
- Mini Mu bacteriophage
albano renee
- Q: purifying lambda phage from extraneous DNA
Hiranya Roychowdhury
- electrophoresis at pH 8,0
Alexander N. Kukushkin
- Silver Staining Reveals Contaminating Bands in Sample
Buffer
Huang Ke-xue
- Calcium Phosphate Transient Transfection of NIH/3T3???
Alexander N. Kukushkin
- painful gel loading???!!!!!!
natldiagnos at mindspring.com
- acid enzyme substrate??
ITABAJARA DA SILVA VAZ JUNIOR
- superantigen genes.help.alternative method to Southern hybridisation sought
Frans and Magda van Gils
- unsubscribe
kwilson at DARWIN.UCSC.EDU
- antigenic algorythms software/Fusion prot.s
Ross Turbyfill
- superantigen genes.help.alternative method to Southern hybridisation sought
Frans and Magda van Gils
- Q: How to remove small size RNA from total RNA in bacteria?
Jinling Xie
- Laboratory Robotics Interest Group meeting
Andy Zaayenga
- Silver Staining Reveals Contaminating Bands in Sample
David C. Logan
- Stratagene QuikChange mutagenesis?
Greg
- FOA problems-Help
John Stebbins
- LA PCR smuck
Wayne M. Barnes
- help-stable horse blood agar-recipes
Michael Heuzenroeder
- Cleaning up Transcriptions
Alexander N. Kukushkin
- DNA recovery by phenol/chloroform extraction or GeneClean
Wilson
- Enhancement of HRP substrate systems??
Joep
- Stripping Northerns
Martin Cann
- Error rate in Taq pol. 1:270?
derk at hubrecht.nl
- Streptavidin and Biotin interactions
Diane Webster
- T4 RNA Ligase
Thomas J. Albert
- PCR/cloning of bacterial communities
Bill Burnett
- Aliquoting Antibodies for freezing - HELP!
Pete
- Antibody against GFP?
David C. Logan
- RNA mobility shift assay.
Morten Andreas Geday
- Seperation of NAD and nicotinamide by HPLC
Heiko Koch
- ssDNA (M13) extract. with silica?
Pascal Mertens
- Cleaning up Transcriptions
brett
- fun with footprinting
Heidi Moss
- A solution for DNA or RNA contamination
Dnacleaner
- The solution for contamination
Dnacleaner
- Problems of contaminations? Think DNACleaner...
Dnacleaner
- PCR, NASBA, bDNA. Contaminations disturb your works?
Dnacleaner
- Simple, economic, DNAcleaner: a solution for contaminations
Dnacleaner
- You use frequently PCR or other technics...This is for you!!!
Dnacleaner
- DNAcleaner, french laboratories already use it against contaminations.
Dnacleaner
- mean stacking temp. algorithm
Les Hoffman
- RNA extraction from small samples...
Hiranya Roychowdhury
- painful gel loading???!!!!!!
martin LEACH
- Where to buy E. coli NP66 for in vivo phagemid release
Christian P. Sommerhoff
- Pierce ECL and BioRad's phosphoimager system?
Robert W. St. G. Fisher, IV
- adult-specific transgene promoter; dimattia@julian.uwo.ca
Gabriel E. DiMattia
- Chromosomal DNA carry-over in plasmid mini-preps
Michael Mangan
- pCR-Blunt vs pCR-Script
bernielu at ERE.UMontreal.CA
- Fusarium & NORTHERN BLOTS
Cristina Placinta
- [Q] : insect hemocyte culture
Min-Ho Lee
- Oligos: Who is cheapest?
cynthia stryker
- sticky surfaces (fwd)
Pradeep M Joshi
- Chromosomal DNA carry-over in plasmid mini-preps
martin LEACH
- Using a PC as an assay counter-timer...
Richard T. Timmer
- SEARLE/MONSANTO JOINS ELECTRONIC NOTEBOOKS CONSORTIUM TO
DEVELOP SYSTEMS TO PROTECT AND MANAGE INTELLECTUAL PROPERTY RECORDS
FOR R&D
Rich Lysakowski, Ph.D.
- SEARLE/MONSANTO JOINS ELECTRONIC NOTEBOOKS CONSORTIUM TO
DEVELOP SYSTEMS TO PROTECT AND MANAGE INTELLECTUAL PROPERTY RECORDS
FOR R&D
Rich Lysakowski, Ph.D.
- ZAP systems
F Dong
- shrimp allergen
Huangxu at space.ihw.co.cn
- SEARLE/MONSANTO JOINS ELECTRONIC NOTEBOOKS CONSORTIUM
Lysakowski at aol.com
- SEARLE/MONSANTO JOINS ELECTRONIC NOTEBOOKS CONSORTIUM
Lysakowski at aol.com
- Thermal Cyclers, Evaluation
Mark Hogben
- HELP: where to obtain rat small intestine cDNA library??
Guido Hooiveld
- subscribe
Henry A. Daum, III
- subscribe
Henry A. Daum, III
- Need gp160 clones for HIV IIIB, MN, BRU
luscher at immune.med.utoronto.ca
- Problems with One Shot INValphaF' from Invitrogen
sunfish
- How many exonucleases have been discovered?
Alexander N. Kukushkin
- How many exonucleases have been discovered?
Ludvig Mortberg
- Stratagene QuikChange mutagenesis?
Huang Ke-xue
- Sequence of pT7-5
Philip Heacock
- pCYPAC2
jcorral at gm.iro.es
- Problems with One Shot INValphaF' from
Invitrogen
Martin Cann
- Screening for Amp(s) bacteria.
Rafael Maldonado
- pGL3 / pGL2 dilemma: anybody working with these ?
Alt Zantema
- Room Sharing at 41st Biophysical Meeting
Newswatcher
- what kind of film?
Michael Fitch
- PCR-Select cDNA Subtraction Kit
Victor Han
- DD-RTPCR
Victor Han
- Inosine in degenerate primers
Dr. Douglas A. Johnson
- Effect of DMSO on dsDNA?
Jerry Kropp
- Screening for Amp(s) bacteria.
Paul N Hengen
- anti-vinculin antibody
u.381 at inserm.u-strasbg.fr
- how to identify a gene on human chromsome 11
K-John Cheung
- Problems with synthetic gene synthesis
Gijs van Rooijen
- Cryostat sectioning...
Jen Robertson
- Problems with synthetic gene synthesis
The Great American Gene Company
- Anyone have a source for bovine cTnT?
Jil Tardiff
- Hirt Extractions
Lawrence Mayer
- (none)
Hung-yiu Ho
- (none)
Hung-yiu Ho
- test...
Gary Kucera
- Reference needed
Dr Banwari Lal
- Molecular clone of dehydroepiandrosterone
Mary P. Remington
- How to purify periplasmic fraction?
Hayssam Zakaria
- Collaboration offered: non-LTR retrotransposones study
Alexander Blinov
- Operon primer sequences
Anita Burger
- "Oligonucleotide purification method??"
Balwi
- pAb against GFP / EGFP
Gunther Rezniczek
- Checking labelling oligo
Balwi
- nylon mesh for hybridizations
Marc Cortese
- what kind of film?
Alexander Kraev
- Reference needed
Elise Palmer
- plasmid DNA needed (50-100 mg)
Paul N Hengen
- PCR and poly a sequences. - wai1.doc [1/2]
wlkl1j at clinmed.gla.ac.uk
- 2mL Concave Gradients???
Lou Cantolupo
- PCR and poly A sequences,
Wai Kwong Lee
- beads for cell culture
xxx
- Immunoblotting on colony lifts
Stephane Pasteau
- Plant m-gfp5-ER (Haseloff)
Mark Cock
- Smearing of PCR product
XIAO-MIN SU
- I need graphics of the number of IgG (y) in function of time (x)
ballet at enib.fr
- beads for cell culture
Jess H. Leber
- RNA Splicing Software
Jose Gonzales
- Limited digestion
Daniel Auerbach
- Are these primer dimers?
Hopkins, Charlotte
- Spectrophotometers
Karl A. Wilson
- GFP expressing cell lines
Jean-Philippe Hugnot
- PCR Techniques Workshop
Mr. Ken Carter
- rDNA Techniques Workshop
Mr. Ken Carter
- Quantitative RT-PCR
J Kennie
- FITC-Guard source?
Richard Vernell
- Application notes on respirometery of living organisms
JanCzek
- epitope tagging
Toai T. Nguyen
- DNA analysis software
WILL H SCOVILL
- test
Richard Daines
- CapFinder PCR cDNA library construction kit
swathi padmanabhan
- Disposal of Hoechst waste
J. G. Gibbings
- plasmid DNA needed (50-100 mg)
Vincent Mulholland
- Operon primer sequences
Dr Alok Adholaya
- GFP/time-lapse
Neil Hotchin
- in search of "Stains-All" staining protocol
doering_t at mpimg-berlin-dahlem.mpg.de
- Amplification from a single algal colony, any thoughts?
Airlie Sattler
- Looking for ColV plasmid
Steven Goldberg
- Application notes on respirometery of living organisms
JanCzek
- Quick transformation protocol wanted
Charles Brockhouse
- stability of GFP in E.coli?
Wolfgang Schechinger
- Q: How to remove small size RNA from total RNA in Bacteria?
Jinling Xie
- Mechanism of EMS Action?
Namjin Chung
- Request for pUC7
Miss S.L. Stowell
- Non-specific binding of avidin woes.
CLARKEY
- Quick transformation protocol wanted
Troels Wind
- Transposon mutagenesis
john.sokatch at CCLINK.NET.UOKHSC.EDU
- A freezer inventory software?
vcore
- XGAL & IPTG -- how low can you go?
David L. Haviland, Ph.D.
- HELP:PAGE Probes
Steve Scherer
- optimizing RT-PCR?
Stefano casalotti
- XGAL & IPTG -- how low can you go?
martin LEACH
- Q: How to remove small size RNA from total RNA in Bacteria?
martin LEACH
- Quick transformation protocol wanted
Jeremy Marcus
- Mechanism of EMS Action?
martin LEACH
- in search of "Stains-All" staining protocol
Hiranya Roychowdhury
- XGAL & IPTG -- how low can you go?
David L. Haviland, Ph.D.
- Quick transformation protocol wanted
A.P.N. de Boer
- Lamda phage - RIP?
David L. Haviland, Ph.D.
- 3'RACE PCR
czlq at musica.mcgill.ca
- GCG
Gurcharan Pahwa
- Transfection
Steve Cohen
- Human cDNA libraries for two-hybrid system
Sanjeeva Reddy
- LPS-free DNA
Thomas Adam
- Lamda phage - RIP?
martin LEACH
- availability of cell lines with t(11;14) translocation
Alex Dobrovic
- Low infection of WEHI231 cell line with eco/ampho retrovirus
tim
- (none)
cjvs at PUBLIC.CC.JL.CN
- Wheat germ agglutinin staining.
A.D. Delduca
- Transfection
brett
- Test..Just Ignore
Chang-Muk Lee
- Circular permutation assay
subtilis
- Phi 29 DNA polymerase
Gordon Munro
- protein phosphorylation
wellman
- 2D peptide mapping
Roney Graf
- Are there single letter codes for phosphoamino acids
Peter Friedhoff
- Transfection
Lou Cantolupo
- IL-4 assay on PBMC, help !
Jens Rainer Hansen
- paraformalduhyde fixation and membrane integrity
Andrew Doherty
- paraformalduhyde fixation and membrane integrity
Richard P Grant
- inositol phosphate enzymatic hydrolysis
Michel RIVIERE
- rubidium chloride for making competent cells?
Sai
- yeast two hybrid
R. Woodward
- Real time PCR measurement
Dr. J.P. Clewley
- protein's pI
czlq at musica.mcgill.ca
- direct genomic sequencing
hendrikb
- mutation analysis
hendrikb
- Fusion Protein Expression Systems
Steve
- Has anyone tried SNuPE ?
Bernard Murray
- protein's pI
Randy Willis
- Fusion Protein Expression Systems
Randy Willis
- FLAG epitope in yeast
Jose Bonner
- Sequencing????
Darren Mark Manley
- Fusion Protein Expression Systems
Ian A. York
- electrophoresis units recommendations?
peter
- Are there single letter codes for phosphoamino acids
Shaun D. Black
- Inexpensive Oligo Purification?
Namjin Chung
- 3' RACE PC
Dr. N.A. Salazar
- how much lambda vector do I need to use
Ken Soderstrom
- luciferase
Your Name
- Inexpensive Oligo Purification?
The Great American Gene Company
- Calcium Phosphate Precipitation
mennow at ruly46.medfac.leidenuniv.nl
- large amount of mRNA is needed
hendrikb
- Disposal of Hoechst waste
Wolfgang Schechinger
- A killer is needed
Alex Borovkov
- (none)
Matthew David Petroski
- My biology site (fwd)
Sudeesha Kunjibettu, by way of C. S.
Prakash
- Fusion Protein Expression Systems
Huang Ke-xue
- How can I assay translational rate ??
u8251012 at cc.kmc.edu.tw
- translational control ??
Yang Y.L.
- translational control ??
Karl Fischer
- e.mail subscription
Rubina Shafi
- AFLP plant mapping kit
Jimmy
- Transposon mutagenesis
Dr Banwari Lal
- Soluene-350 composition/source?
Mark
- IMPACT
Victor Han
- cDNA Library
Patrick HJ Falckh
- Peptide nucleic acids (PNA)
Ludla
- pEE14
Bjšrn Olde
- myelin
Sandra Supraha
- myelin
Sandra Supraha
- Translational control
Mr. G. Morley
- Help! PCR linearity
Joanne Rogers
- Help! PCR linearity
Joanne Rogers
- Ecdysone Inducible System
Chris Williams
- Invitation to BioMOO
Gustavo Glusman
- Washing slides for in situs
GILLIAN NI BHARLO BOTANY PG
- HELP:PAGE Probes
Wolfgang Schechinger
- Calcium Phosphate Precipitation
brett
- Kozak concensus sequence
jovermeyer at shrsys.hslc.org
- PCR detection of RETROVIRUSES
Raymond Henderson
- Antisense transcripts
Zhiyong Zhao
- (none)
vduke at ANCON.UP.AC.PA
- (none)
vduke at ANCON.UP.AC.PA
- Stop codon read-through?
Harris Laboratory
- Histone H1 size?
Rizwan Haq
- E. coli (Bl21DE3) lyses automatically
Huang Ke-xue
- Gene organization
Sigurd Ørstavik
- epitope mapping kit
Jimmy Loh
- Kozak concensus sequence
brett
- acid phenol extractions
sunfish
- E. coli (Bl21DE3) lyses automatically
Greg
- 293 cells in serum-free medium?
Lloyd Graham
- Knockout mice advice
Karen
- Knockout mice advice
Karen
- WANTED: Src construct
Mr. G. Morley
- RNA Ligase
Matt Thomas
- Kozak concensus sequence
Deborah A. Samac
- address
Jenny Allsop
- Help suppliers of biotinylated magnetic beads
a.molloy
- AATAAA signal
czlq at musica.mcgill.ca
- acid phenol extractions
brett
- IMPORTANT: Eukaryotic vector sequences!
Ole Gjoerup
- single tube nested pcr
gzk at ollamh.ucd.ie
- Transient transfection of Cos-7 (electroporation v DEAE dextran)
Kevin A. Mulcahy
- Request for integrin plasmids or antibodies. PLEASE.
CLARKEY
- fluorescent sequencing of cosmids
David D Morgan
- anti-prion protein
Julie Roberts
- Protein Expression
Klaus Lun
- Protein Expression
Klaus Lun
- E. coli (Bl21DE3) lyses automatically
Albert Crescenzo
- How much thrombin for GST fusions ?
Tom Haenel
- multiplying bands in gel purification
John G. Luz
- pH of water; dissolving RNA
Hildur V. Colot
- Monoclonal vs polyclonal antibody
Levon Fendekian
- Low RNA yield after Qiaquick column purification.
Zhongtang Yu
- Anyone Using Competimer (TM) Technology for RT-PCR?
Richard Rohan
- expression vector problem
wliu at receptor.pharm.uwa.edu.au
- pH of water; dissolving RNA
Alex Dobrovic
- acid phenol extractions
Ben Haskell
- removal of PEG from DNA disolved in TE?
Venkat Pisupati
- How much thrombin for GST fusions ?
Curt Ashendel
- pH of water; dissolving RNA
Curt Ashendel
- nonspecific primer extension products
Barr Lab
- Strange contaminant in cell culture
Obaid Y. Khan
- learning to use newsgroup
boukhb01 at mcrcr6.med.nyu.edu
- removal of PEG from DNA disolved in TE?
Richard P Grant
- Q:Measurement of protein's surface hydrophobicity?
Achim Recktenwald, PhD
- Cosmid cloning recommendations?
Volker Knoop
- refolding from inclusion bodies
juhi juneja
- Scanalytics RFLPscan
David Ley
- Strange contaminant in cell culture
Richard P Grant
- dam-/dcm- bugs and mutations??
Dr. Duncan Clark
- Strange contaminant in cell culture
brett
- Westerns and estrogen receptors
Aideen O Doherty
- SR-B1 (scanvenger receptor B1) required
Benoit Gauthier
- refolding from inclusion bodies
Gouzel Karimova
- DNA-quantitation
Joern Schwede
- refolding from inclusion bodies
bipin k. dalmia
- no-BglI plasmid
Peter Cheung
- 3-Amino triazole conc.?
Martin Gossen
- Free webzine for BioMedNet members
Huang Ke-xue
- Conductance Standards
Leon Scott
- Strange contaminant in cell culture
Dima Klenchin
- DNA sequence comparision
wjc at acsu.buffalo.edu
- Deglycosylation of proteins: questions
Dale Karlson
- GFP S65T vectors
Hildur V. Colot
- E. coli (Bl21DE3) lyses automatically
Huang Ke-xue
- ?-easiest clean-up for sequencing
Tom Duda
- epitope mapping kit
Ross Turbyfill
- Monoclonal vs polyclonal antibody
Ross Turbyfill
- multiplying bands in gel purification
Jill Kellogg
- refolding from inclusion bodies
Randy Willis
- DNA quantitation
Dick Haugland
- Q:Measurement of protein's surface hydrophobicity?
"Uncle Al" Schwartz
- Stable Thp1 transfections
Guriq Basi
- Q: Source of terminating ribonucleotides
Igor Sidorenkov
- 5'-psoralen-labelled primers
Karel Wernars
- Custom DNA-Sequencing in Europe
FABIAN MOEBIUS
- northern blotting
a.mccormick
- hyperfilm-MP/hyperfilm-ECL
Federici
- A review on bacterial promoter consensus sequence
Jean-Francois Mayaux
- SDF-1 antibodies to get or buy
Morgane BOMSEL
- SDF-1 antibodies
Morgane BOMSEL
- S.aureus primers
laban
- Long PCR & Joining 5' of sequence to the rest
Harry Witchel
- Reamplification pcr products after Ag-staining
Michael Vork
- biometra vs perkin elmer thermal cycler
Di James
- Available statistical programs
Mark Lamkin
- Pepsin: IU to FIP unit ????
H Chang
- protein solubility problems
Malcolm M. Campbell
- double labeling with BrDU
Danny Altschuler
- Wanted: Tie2 expressing cell line.
XXX
- YAC libraries
J. G. Gibbings
- Wanted: Tie2 expressing cell line
Medvinsky
- northern blotting
Bernard Murray
- Sequencing and sharkteeth
Jørgen Stenvik
- Protein Extraction Buffers...What's a good homogenization buffer for westerns?
Rob Wesley
- differentiating between genomic & plasmid DNA
E.Hillery
- double labeling with BrDU
Jorg Kirberg
- hot PCR
Caroline Szymeczek-Seay
- Desire recommendation re 2-D gel apparatus
Jerry Kropp
- trypsin magnetic beads
Mick Foley
- Jurkatt cell induction
rayj
- Immunohistochemistry help
melvnr at hg.uleth.ca
- hyperfilm
Federici
- SSC conc. in prehyb.
Denni Schnapp
- N-terminal sequence
Emile Schiltz
- Tris base vs. Trizma
Denni Schnapp
- screening of cDNA library: custom services
Myriam Onno
- Molecular modelling capabilities
Dr. Duncan Clark
- differentiating between genomic & plasmid DNA
Hiranya Roychowdhury
- Help: Bacterial Genomic DNA extraction and library construction
Joel Bader
- Geneskipper vs DNASIS
FABIAN MOEBIUS
- Primer design
Yang Y.L.
- Taq polymerase inhibition reference
Michael Douglas
- Proteinase K and detergent
Jill Kellogg
- test
Ray Boykiw
- subscription to a newsgroup
Francis ROBERT
- Na+/K+ ATPase assay
J Hance
- skatron?
Nancy Linford
- RNase Protection Assay
mennow at ruly46.medfac.leidenuniv.nl
- INDEX MEDICUS address
Jenny Williams
- PYMS Diagram
Jenny Williams
- quick question
Rani Geha
- error rate reverse transcriptases
F.Schaap at FYS.UNIMAAS.NL
- oligo biotinylation?
Yi Zhang (BIO)
- quick question
Hiranya Roychowdhury
- best total RNA and mRNA isolation kits
Karim Hyder
- transfection/transformation
E.Hillery
- LB contains glucose?
hbkg at hitel.kol.co.kr
- Expression of bcl-2 homologue bad
Reinhard Hoffmann
- Stability of Doxycycline?
Stephan Geley
- Q:measurement of protein's surface hydrophobicity
Kurt L Brillhart
- jwc-nondenaturing geo of protein
Loesch-Fries Lab
- phosphatase detection in yeast ...
Daniel O. Espinoza
- Help!Mouse fibroblast cell line dying!
Tori D. Williams
- The Antibody Resource Page
Kevin Shreder
- Hirt extractions
Graeme J. Dougherty
- help with pcr for dna from sediments
Guenter Jost
- BIOSCI/bionet miniFAQ & Fundraiser
BIOSCI Administrator
- mRNA isolation kits for plant culture tissue
nhwu at PUBLIC3.BTA.NET.CN
- PCR
czlq at musica.mcgill.ca
- Gancylovir Supplier
David Hills
- Adhesive temperature indicator / warning for reagants & buffers
Charles_Lasitter
- Antibody against T7 RNA Polymerase
Alain Kohl
- Acetamide in PCR reaction?
Anton Scott Goustin
- pLAFR1 cosmid restr. maps, anyone?
weezer Mccheese
- Where to be published
Michael Almeida
- Na+/K+ ATPase assay
J Hance
- Cutting DNA from non-E.coli
Eileen Hickey
- Antisense oligonucleotides
Sandra Pena De Ortiz
- long PCR with dUTP
Michael C. Gorry
- RNA from urine
nik
- constitutive gene in bacteria
NG WAI LEUNG
- Seeking secretion vector to express MFalpha-fusion protein
James Irving
- Green Fluorescent Protein Purificaton Short Course
Daniel Gonzalez
- WANTED: pTrc99 plasmids
Ed Schwartz
- Adhesive temperature indicator / warning for reagants & buff
Wolfgang Schechinger
- Help on bacterial analysis?
l95mn at river.tay.ac.uk
- Looking for cDNA sequence or accession number of IRS-2
Wolfgang Schechinger
- dot blot with bacteria
Stephane Pasteau
- ATP
SARAH LOUISE RAWLINGS
- rRNA contamination after RNA poli-A purification with oligo-dT
CARLOS ALEXANDRE SANCHEZ FERREIRA - 218
- Kodak Biomax Vs Fuji RX
frogs 'r' us
- PVP/PVPP
Hiranya Roychowdhury
- Q: PCR cloning
Jinling Xie
- test
XiaoYin Zhong
- blotting acrylamide gels
Constable Julie Lynn
- Gridded Membranes
Rory O'Brien. Biotechnology.
- meaning of acronym
Regina Shaw
- Help: cleaning sintered glass funnels
Guy Oshiro
- Sea Urchin and Manduca cDNA libraries
Greg Beck
- blotting acrylamide gels
Michelle Gleeson
- Custom DNA-Sequencing in Europe
Francois Coulier
- RER+ mouse cell lines
Bob Steinberg
- Gridded Membranes
Hiranya Roychowdhury
- Problem with nonspecific binding
DiverNLB
- Mac-based commercial mapping program
GEN282E at CCS1.cc.monash.edu.au
- Looking for cDNA sequence or accession number of IRS-2
martin LEACH
- WANTED: Human Genomic (Cosmid) Library
jxie at bi-pharm.com
- Rodent (mouse or rat) microsat. primers wanted....
Dave Edwards
- (none)
Takeshi SAITO
- Protein-protein interaction of His-tag of pET14b?
Jimmy S.H. Tsang
- **************Gancyclovir**********************
David Hills
- T7select phage display
Liselotte Kahns
- Disposable counting chamber
Eike
- Dissociating agents to prevent protein aggregation
newera at plaza.snu.ac.kr
- Looking for cDNA sequence or accession number of IRS-2
Peter Pichler
- VHD strain request
Stephane Pasteau
- Looking for cDNA sequence or accession number of IRS-2 - whole text
Peter Pichler
- Questions about DNA recovery and cloning
Wilson
- monoclonal antibody for HA
Krista Fehr
- Question about genomic library construction
Wilson
- ?-easiest clean-up for sequencing
David L. Haviland, Ph.D.
- Horseradish Peroxidase assay
Tom Parish/Cambridge/Biogen
- **************Gancyclovir**********************
Toumy Guettouche
- Horseradish Peroxidase assay
Sanjay Krishnaswamy
- TRAP assay
xin wei
- Northern blot RNA degradation
Jos
- Questions about DNA recovery and cloning
Alexander N. Kukushkin
- tempest on a toothpick
Tom Cooper
- Question about genomic library construction
Alexander N. Kukushkin
- Breakthroughs in SPM
Mark Flowers
- Clontech GFP
Alex Harvey
- Source of bismuth subcitrate in N. America?
REW
- TRAP ASSAY
Xin Wei
- WANTED: Vector to express MF-alpha fusion protein
James Irving
- Single Copy Plasmids
Brett Beitzel
- microsatellite loci amplification
kfeldh1 at ICARUS.CC.UIC.EDU
- PCR contamination
Stsarev
- yeast 2 hybrid screen on heart cDNA library
Malathi Hari
- does anyone have a spac-promoter vector
Neil Mattatall
- How to use BLAST
Zhencai Wu
- Making Nickel resin??
Stephen W Doughty
- Apparati for Northern Blotting
DiAnna L. Hynds
- Questions about DNA recovery and cloning
Hiranya Roychowdhury
- PCR contamination
Dr. J.P. Clewley
- Primary embryo fibroblasts transfection
Rizwan Haq
- Agrobacterium
M Allen
- Agrobacterium
M Allen
- Molecular Modellers - Wanted
Michael Parkinson
- Question about genomic library construction
Huang Ke-xue
- ADVANE IMMUNOFLUORESCENE COURSE
Paolo Castano
- T7 or SP6 RNA Pol promoter sequence
Gordon Munro
- Test Post
Ed Wang
- Mycoplasm test by PCR
S Von
- Making Nickel resin??
Curt Ashendel
- Single Copy Plasmids
Karl Fischer
- hydroxymethyl dCTP
Les Hoffman
- Cambridge Healthtech Institute’s MOLECULAR EVOLUTION
Cambridge Healthtech Institute
- Transformation protocol for Bacillus needed
Arul Jayaraman
- A BiaCore-like system
Randy Willis
- (none)
Benoit Gauthier
- guanylyl cyclase antibody
m hayden
- 2-D software for Mac?
Jerry Kropp
- RNA secondary structure
A. Kumar
- Random peptide library
Richard Vernell
- Hot plasmid + Cold Vector = Insert-specific probe????
Michael J. Prigge
- integrin antibody
ANNE GEORGE
- Best commercial Factor Xa?
alan friedman
- Hirt procedure pl.
Patil J G
- Help
Guy McDonnell
- Adhere cells onto slides for immunostaining
Susan Hubchak
- Adhere cells onto slides for immunostaining
Zheng Huanquan
- degenerate PCR
Dr Koen A.L. De Smet
- transgenic bacteria
Stephane Pasteau
- Biorad Quantum minipreps re ALF sequencers
Oliver S. DePeyer
- Ph.D. Assistantship Available
Dr. Jeffrey Dean
- Q: Clones from genomic libraries?
Bart Nelissen
- heart 2hybrid screen
Malathi Hari
- Methicillin suppliers?
Derron Blakely
- Dexamethasone-inducible pMAMneo vector
Marit Anthonsen
- How to separate vegf dimer into monomer?
Ziping Yang
- gel doc/photo imaging system
Hoyun_Lee at octrf.on.ca
- Sectioning mouse eyes Help!
Kevin Rattray
- Effect of Phenol degradation prodcust on bacterial bioluminescence
corbisip at vito.be
- biotinylation of BBMV
Edwin Alcantara
- Check
Shiwani Arora
- A little help here...
The Kindly One
Last message date:
Fri Feb 28 22:10:36 EST 1997
Archived on: Sun Mar 6 21:22:10 EST 2005
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