Q: NHS acivated Agarose (Sepharose): (in)compatitibilities?

Wolfgang Schechinger wgschech at med.uni-tuebingen.de
Thu Jan 2 04:50:00 EST 1997


Dear Bionetters, 

I would like to link a protein to agarose, using the N-hydroxy succinimide
activated sepharose columns from pharmacia. Since I need to dissolve my
protein from inclusion bodies with a buffer containing urea or guanidinium
salts, I'm afraid that this stuff will deactivate the activated column. 

you have any experience or suggestions?


Wolfgang




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