Construct Microinjection

Sandra Pena De Ortiz spenadeo at
Thu Jan 2 12:23:23 EST 1997


I am planning to generate transgenic mice expressing a protein of 
interest under  the control of Tetracycline.  In these experiments people 
usuaklly generate two transgenes; one expressing tTA and the other with 
the regulated gene and then cross the mice to obtain animals that have 
both contructs.  Would it be feasible to inject two constructs 
simultaneously and obtain animals with both constructs this way?

Thanks for any coments

Sandra Pena de Ortiz, Ph.D.
University of Puerto Rico

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