Bacteriophage concentration

Dr. Duncan Clark duncan at
Thu Jan 2 11:12:40 EST 1997

Hi Folks,

I have a bateriophage that appears very sensitive to lysis. Pptation
with PEG/NaCl causes it to lyse. Running an aliqout of the phage lysate
on a TBE agarose gel also causes the phage to lyse. I need to
concentrate it as the highest titre one can ever get is about 10E8 per
ml. I haven't yet tried putting the lysate in dialysis tubing and
concentrating against solid PEG but that may be worth a go.

Any alternative pptation methods out there?

Many thanks

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288

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