how to remove SDS from RNA sample?

Federici federici at icgm.cochin.inserm.fr
Thu Jan 2 10:23:03 EST 1997


In article (Dans l'article)
<Pine.A41.3.95b.961224133143.73648A-100000 at dante13.u.washington.edu>,
Seng-Lai Tan <sltan at u.washington.edu> wrote (écrivait) :

> Dear fellow researchers,
> 
> i'm trying to reverse transcribe my RNA but it's resuspended in water that
> contains some SDS, which is inhibiting the RT reaction.  Does anyone know
> of a easy method (e.g. precipitation) that I can use to remove the SDS in
> my sample?  many thanks in advance.
> 
> seng-lai

cetyl methyl ammonium bromide (CTAB) binds to SDS (1 mole /1 mole) at 37°C
(30mn). SDS-CTAB complex can be eliminated by centrifugation (10000g, 1hr,
20°c).



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