protein help needed

lachlan harris lachlan.harris at jcu.edu.au
Sat Jan 4 17:21:14 EST 1997


Can anyone help me with this problem? I have a pure microbial protein 
which when run on an SDS non-reduced (ie no mercaptoethanol) PAGE gel 
runs at approximately 50 KDa. When the same protein is run under 
SDS-reduced conditions two subunits appear- one running at approx 40 KDa 
and the other at approx 60 KDa.

It was my understanding that the two subunits should add up to be the 
same size as the protein run under SDS-non reduced conditons. But this 
isn't happening. Does anyone know why?



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