Q: Does blunting w/ Klenow make PNK phosphorylation obsolete?
drm21 at mole.bio.cam.ac.uk
Sun Jan 5 11:44:30 EST 1997
In article <5aofsf$k5h at mserv1.dl.ac.uk>, Wolfgang Schechinger
<wgschech at med.uni-tuebingen.de> wrote:
>Does it? or does it not?
>Anny suggestions are welcome!
It doesn't. PNK puts on a _5'_ phosphate. Klenow will not affect the 5'
end of your DNA at all: 5' overhangs are _filled in_ by extending the
3'-OH of the other strand until the two strands are the same length. 3'
overhangs are chewed back from the 3'-end.
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