No plasmid from Miniprep

Dr. Duncan Clark duncan at genesys.demon.co.uk
Mon Jan 6 04:13:13 EST 1997


In article <zjons-0501971134560001 at 130.60.120.18>, "Zophonias O.
Jonsson" <zjons at vetbio.unizh.ch> writes
>Additionally the pET's are low copy number so one will always have rather
>low yields.  I grow them in ultra rich, well buffered medium (Superbroth)
>for minipreps.

As they are based on pBR322, they can be amplified with chloramphenicol
or spectinomycin.

Duncan
--------------------------------------------------------------------------------
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Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288
http://www.dnamp.com
http://www.genesys.demon.co.uk



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