No plasmid from Miniprep

Dr. Duncan Clark duncan at
Mon Jan 6 04:13:13 EST 1997

In article <zjons-0501971134560001 at>, "Zophonias O.
Jonsson" <zjons at> writes
>Additionally the pET's are low copy number so one will always have rather
>low yields.  I grow them in ultra rich, well buffered medium (Superbroth)
>for minipreps.

As they are based on pBR322, they can be amplified with chloramphenicol
or spectinomycin.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288

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