Removal of E.coli non-specific binding

Pascal Mertens Pascal.Mertens at
Mon Jan 6 10:36:12 EST 1997

In article (Dans l'article)
<01bbf68b$58a33a80$cd7106c2 at>, "DAVID
PULFORD" <david.pulford at> wrote (écrivait) :

> I'm looking for a quick and easy way of removing E.coli antibodies from a
> rabbit serum. I've found pseudoscreening an incomplete, time consuming and
> expensive way of removing this background activity for screening an E.coli
> expression library. I chanced upon a method a while back that used
> formalised cells but have no reference for this approach. Can anyone help?

There was a detailed protocol some months ago in BioTechniques with both
formalized and autoclaved bacteria.

Pascal Mertens
Laboratoire d'Immunologie et Microbiologie
61 Rue de Bruxelles
5000 Namur, BELGIUM
Phone: 32 81 724438
Fax: 32 81 724420

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