Removal of E.coli non-specific binding

Pascal Mertens Pascal.Mertens at fundp.ac.be
Mon Jan 6 10:36:12 EST 1997


In article (Dans l'article)
<01bbf68b$58a33a80$cd7106c2 at david.pulford.pop-3.ukonline.co.uk>, "DAVID
PULFORD" <david.pulford at ukonline.co.uk> wrote (écrivait) :

> I'm looking for a quick and easy way of removing E.coli antibodies from a
> rabbit serum. I've found pseudoscreening an incomplete, time consuming and
> expensive way of removing this background activity for screening an E.coli
> expression library. I chanced upon a method a while back that used
> formalised cells but have no reference for this approach. Can anyone help?

There was a detailed protocol some months ago in BioTechniques with both
formalized and autoclaved bacteria.
Pascal

-- 
Pascal Mertens
Laboratoire d'Immunologie et Microbiologie
URBM-FUNDP
61 Rue de Bruxelles
5000 Namur, BELGIUM
Phone: 32 81 724438
Fax: 32 81 724420



More information about the Methods mailing list