Prepping Plasmids for Automated Sequencing?
"Marianne Leverone ", BIO
leverone at CHUMA.CAS.USF.EDU
Mon Jan 6 16:36:48 EST 1997
Pat: The Qiagen columns work well. We use midi (~100 ug yield). It is
important to use a good host: E. coli DH5 alpha works well but we have
not had luck with E. coli JM 109. Marianne
On Fri, 3 Jan 1997, J. Pat Martinez wrote:
> Any suggestions on miniprep columns for purifying plasmid DNA to be used
> for automated sequencing? I was considering buying the mini prep spin
> columns from Qiagen or the perfect prep columns from 5 prime 3 prime.
> Pat M.
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