PCR help: D2S 120; won't amplify

John Wolff wolffie at u.washington.edu
Tue Jan 7 17:34:40 EST 1997


This is a Weissenbach marker.  I'd appreciate any help from anyone who's
used it successfully.

We're getting no amplification using conditions listed in the GDB.

96 deg.       5 min initial soak (also tried without this step)
94 (or 92)   30 sec
55 deg       30 sec
      35 cycles    
72 deg       10 min
         
25 uL volume, 1.5 mM Mg++, 100 ng DNA template, 0.3 uM or 1.0 uM primers



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