Sup F selection ?

Ashok Aiyar aiyar at ebv.oncology.wisc.edu
Wed Jan 8 12:20:38 EST 1997


On 8 Jan 1997 16:26:55 GMT, j.langner <j.langner at dkfz-heidelberg.de> wrote:
>Who knows which E.coli strain to use for Sup F selection of plasmids?

There are several auxotropic strains that can be used for supF
selection.  I prefer to use a strain called MC1061/p3 which contains
an episome, p3, that has Amp-amber and Tet-amber genes.  A simple
Amp/Tet selection is sufficient to select for the supF containing plasmid.

MC1061/p3 is available from Invitrogen.  I think that BRL also sells a 
strain with the p3 episome called DH10B/p3.

Ashok
-- 
Ashok Aiyar, Ph.D.
McArdle Laboratory for Cancer Research
University of Wisconsin-Madison



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