Use of rTH polymerase in RACE

John Morrow johnm at am.alba.umc.akzonobel.nl
Wed Jan 8 09:04:32 EST 1997


I'm considering using rTH polymerase for the reverse transcription step of a 5'
RACE experiment in order to overcome the high GC content at the 5' end of the
gene I'm working on.   Does anyone out there have any experience with this, and
if so, could you send any protocols please?

Thanks,
John



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