hybond

Szabadkai Gyorgy SZABADKAI at PUSKIN.SOTE.HU
Wed Jan 8 07:03:51 EST 1997


Hi,

I tried to use Hybond N membranes for dot blots of RNA and DNA, but 
the DNA signal has lost somehow. I denaturated the DNA by boiling for 
5 minutes and then fixed on the membrane by UV crosslinking (1200 
mJ/cm2). The probe was ok, I checked it.
What kind of denaturation and fixation do you use for this memebrane?


Szabadkai Gyorgy



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