Sup F selection c

[Ian A. York]

In article <slrn5d7lr6.j13.aiyar at ebv.oncology.wisc.edu>,
Ashok Aiyar <aiyar at ebv.oncology.wisc.edu> wrote:
>
>MC1061/p3 is available from Invitrogen.  I think that BRL also sells a 
>strain with the p3 episome called DH10B/p3.

DH10B/P3 gave me much smaller (slower-growing) colonies than did
MC1061/P3.  I don't know if DH10B/P3 have some advantage over MC1061/P3 in
other ways.

Electrocompetent MC1061/P3 is also made by Clontech labs; I found that the
transformation efficiency of Clontech's was about 5-fold better than
Invitrogen's, and about twice as good as my home-made electrocomp
MC1061/P3. 

I use Amp/Tet selection for the SUpF plasmids as described by
Invitrogen.

No affiliation with any of the above. 

Ian 
-- 
      Ian York   (iayork at panix.com)  <http://www.panix.com/~iayork/>
      "-but as he was a York, I am rather inclined to suppose him a
       very respectable Man." -Jane Austen, The History of England