Does CIAP cause ligation problems?

Fiberhead stern100 at mail.concentric.net
Thu Jan 9 17:11:40 EST 1997


>Hi everybody,
>
>For the last two months I am trying to perform a non-directed ligation. 
>Therefore I tread my vector with CIAP (Promega). However, till now I
>wasn't 
>successful.
>

For what its worth, it has been my experience that inactivation of CIAP can
be the culprit.  I think we routinely heat at 75 degrees in the presence of
EDTA for at least 10 minutes AND phenol extract.

-seth





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