Does CIAP cause ligation problems?
stern100 at mail.concentric.net
Thu Jan 9 17:11:40 EST 1997
>For the last two months I am trying to perform a non-directed ligation.
>Therefore I tread my vector with CIAP (Promega). However, till now I
For what its worth, it has been my experience that inactivation of CIAP can
be the culprit. I think we routinely heat at 75 degrees in the presence of
EDTA for at least 10 minutes AND phenol extract.
More information about the Methods