Mammalian cell transfection

Tony Hodge tph at mrc-lmb.cam.ac.uk
Fri Jan 10 06:23:24 EST 1997


Mike Garbutt <mgarbutt at gpu.srv.ualberta.ca> wrote:

> LABO DE BIOCHIMIE wrote:
> > 
> > Hi folks,
> > I am planning to transfect mammalian cells by using the classical
> > calcium phosphate procedure with the recombinant expression vector
> > pCI-neo (Promega), which confers by its own G-418 resistance to
> > transfected cells.
> 
> I would suggest using "DOSPER" and do transients if you can get away with
> it; you can get almost 100% transfection efficiency, as long as you don't
> kill all your cells :-(
> Mike

OK call me thick but what is DOSPER, references etc please.

tia

tony


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Tony P Hodge                              MRC-LMB, Hills Road 
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