Subcloning PCR products

Christian Neri neri at cephb.fr
Fri Jan 10 04:41:57 EST 1997


Joy Richman wrote:
> 
> Dear Netters,
> 
> I would like to subclone some PCR products as quickly as possible. I am
> prepared to spend money on a kit but would like some reviews on the kits
> that are out there. Also a comparison of the blunt ended, TA cloning and
> cloning using specific primers provided with the kit would be useful.
> 
> Thanks very much for your help.
> 
> Joy Richman


One highly efficient and easy way of subcloning PCR products is to use
the UDG system from Life Technologies. One would just need to use
primers carrying a (AUG)4 tail at their 5'end. In our hands, this system
is better than TA cloning.

Chris Neri



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