Long PCR Primers
Sandra Pena De Ortiz
spenadeo at lonestar.jpl.utsa.edu
Fri Jan 10 16:15:42 EST 1997
I am thinking on using a 40 bp long pcr primer. I need to add a
consensus Kozak sequence for expression and want to add a bamhi site to
ease cloning. Does anyone know if a primer this long will work? The
longest I have tried is 26 and had no problems. Also, how many bp minimum
should
I add 5' to the bamhi site to ensure binding and cleavage by the enzyme?
Thanks in advance,
Sandra Pena de Ortiz, Ph.D.
Dept. of Biology, UPR
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