Long PCR Primers
Sandra Pena De Ortiz
spenadeo at lonestar.jpl.utsa.edu
Fri Jan 10 16:15:42 EST 1997
I am thinking on using a 40 bp long pcr primer. I need to add a
consensus Kozak sequence for expression and want to add a bamhi site to
ease cloning. Does anyone know if a primer this long will work? The
longest I have tried is 26 and had no problems. Also, how many bp minimum
I add 5' to the bamhi site to ensure binding and cleavage by the enzyme?
Thanks in advance,
Sandra Pena de Ortiz, Ph.D.
Dept. of Biology, UPR
More information about the Methods