I need help removing IgG from protein homogenates!

Bob Steinberg RSTEINBE at ETOWAH.UOKHSC.EDU
Fri Jan 10 21:09:11 EST 1997


Jil Tardiff wrote:
> 
> What I would *like* to do is figure out a way to *remove* the IgG from
> the initial protein homogenate. The protein is isolated from whole mouse
> hearts in a low-conc KCl-KPO4 buffer. And yes, I try mightily to wash
> the blood out before homogenization.
> How about running your homogenates over a protein A-Sepharose column?



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