N-terminal blockage - can it be removed?

Regina Shaw regina at biotech.ufl.edu
Fri Jan 10 16:52:47 EST 1997

Dear Newsgroup -
We would like to get N-terminal sequence data for an antibody but, whereas
the light chain sequenced well, the heavy chain appears to be blocked.
I've heard that there is a chemical that can remove N-terminal blockage in
antibodies but have no reference at all as to what it's called and how to
use it etc. I would very much appreciate any helpful hints.
Thank you very much,
Regina Shaw
Recombinant Protein Expression Core

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