long PCR Thermocycler

soenke behrends behrends at plexus.uke.uni-hamburg.de
Sun Jan 12 07:28:05 EST 1997

Hello methods-reagents people,
I have a question for "long PCR specialists":
Can you recommend any special Thermocycler for long PCR?
Is in your opinion the choice of the Thermocycler critical?
I want to set up long PCR for the first time. 
So I would also be happy about hints which enzyme (or kit) 
to choose, whether thin tubes are vital and whether the choice
and quality of primers is any different than in regular
PCR. Has anyone tried the capillary air cycler for long PCR?


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