TA cloning and competent cells
goldberg at bms.com
Wed Jan 15 09:00:15 EST 1997
In article <Pine.A220.127.116.110113183839.20104H-100000 at aix1.uottawa.ca>,
sunfish <s535290 at aix1.uottawa.ca> wrote:
> Hi all,
> does anyone ever use homemade competent cells for use with TA cloning ?
> (we use the pCR2.1 by invitrogen) Sometimes we run out of the competent
> cells that come with the kit and if I'm in a hurry and can't wait for new
> cells to arrive, it'd be nice to know that the home made stuff is alright.
> If anyone does use their own competent cells, what strain do you use, and
> what method do you use for making the cells competent ?
> thanks in advance,
I will at times have problems with the competent cells from Invitrogen
(either I run out of them or they seem to lose their competency). In such
instances I substitute with home-made electrocompetent INValphaF' or
similar strain such as DH10B from BRL with no problems at all.
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