Calcium phosphate transfection

Tsunehisa Namba drnamba at kuhp.kyoto-u.ac.jp
Tue Jan 14 02:32:14 EST 1997


Hi
I am trying to assess my cDNA construct by transfecting cells with calcium 
phosphate method.  I made 2M CaCl2 solution, Hepes buffered saline (pH 
7.12) and phosphate solution.  It worked fine: 30-40% of the cells got 
transfected.  However in 3 weeks, the efficiency goes down to under 1%.  I 
store Hepes buffered saline at -20 degree.  Others at room temp.
   Any suggestions?  Thanks.

-- 
***************************************************
Tsunehisa Namba
Department of Anesthesia, Kyoto University Hospital
Sakyo-ku, Kyoto 606-01, Japan
Fax:+81-75-752-3259
E-mail: drnamba at kuhp.kyoto-u.ac.jp



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