Choice of mammalian expression vectors?

Darren Tyson tysondr at
Wed Jan 15 19:09:11 EST 1997

Alan Nighorn wrote:
> Hi All,
> We are going to be expressing some cloned proteins in COS-7 cells
> and wondered if any of you folks had any preferences for particular vectors.
> We are also interested in any vector horror stories out there so that we
> might avoid the same fate.
> Cheers
> Alan Nighorn

pCDNA3.1 from invitrogen is my vector of choice for overexpression of
proteins in eukaryotic cells.  They also have variant with different


Darren Tyson
Ph.D. Candidate in Cell and Molecular Biology
Saint Louis University
tysondr at or darren at

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