Choice of mammalian expression vectors?

[Darren Tyson]

Alan Nighorn wrote:
> 
> Hi All,
> 
> We are going to be expressing some cloned proteins in COS-7 cells
> and wondered if any of you folks had any preferences for particular vectors.
> We are also interested in any vector horror stories out there so that we
> might avoid the same fate.
> 
> Cheers
> 
> Alan Nighorn

pCDNA3.1 from invitrogen is my vector of choice for overexpression of
proteins in eukaryotic cells.  They also have variant with different
tags.

Cheers,

Darren
-- 
Darren Tyson
Ph.D. Candidate in Cell and Molecular Biology
Saint Louis University
tysondr at slu.edu or darren at primary.net
http://www.scimart.com/darren/