Delta G values for would-be primers...
Edgar Valencia
edgar at CIFN.UNAM.MX
Wed Jan 15 17:35:03 EST 1997
Dear Oligonovice :)
>
> Can anyone tell me how to interpret delta G values with respect
> to formation of hairpins and dimers?
The most negative delta G value, the most stable hairpin ie the highest
temp to separate the strands and the harder to get a PCR product
Hope this helps
Edgar Valencia Morales
Departamento de Genetica Molecular
CIFN - UNAM
MEXICO
e-mail edgar at cifn.unam.mx
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