Delta G values for would-be primers...

Edgar Valencia edgar at CIFN.UNAM.MX
Wed Jan 15 17:35:03 EST 1997


Dear Oligonovice :)
> 
> Can anyone tell me how to interpret delta G values with respect
> to formation of hairpins and dimers?

The most negative delta G value, the most stable hairpin ie the highest 
temp to separate the strands and the harder to get a PCR product
Hope this helps

                          Edgar Valencia Morales
                    Departamento de Genetica Molecular
                               CIFN - UNAM
                                  MEXICO
                        e-mail edgar at cifn.unam.mx



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