PCR: magnesium vs annealing temperature

barbj at molgennn.demon.co.uk barbj at molgennn.demon.co.uk
Thu Jan 16 13:16:29 EST 1997

Can anyone suggest whether and why magnesium concentration and annealing 
temperature are not simply interdependant in the absence of magnesium 
sequestration. I have never found a PCR that could not be optimised by 
adjusting temperature alone.

Gavin Willis

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