TA cloning and competent cells

Clemens un691cs at genius.embnet.dkfz-heidelberg.de
Thu Jan 16 12:34:54 EST 1997

In contribution <goldberg-1501970857520001 at>, the honourable 
goldberg at bms.com stated:

>In article <Pine.A41.3.95.970113183839.20104H-100000 at aix1.uottawa.ca>,
>sunfish <s535290 at aix1.uottawa.ca> wrote:

>> Hi all,

>> does anyone ever use homemade competent cells for use with TA cloning ? 

all the time, as it is perhaps 1 % of the cost of commercial
ones. the efficiency of my cells is around 1.000.000 colonies
per µg of pUC19, and that's enough for most applications 
(I get every PCR product cloned that I want)


More information about the Methods mailing list