how to remove SDS from RNA sample?

Josh Gamse gamse at
Thu Jan 16 21:48:59 EST 1997

In article <federici-0201971632190001 at>,
federici at (Federici) wrote:

>In article (Dans l'article)
><Pine.A41.3.95b.961224133143.73648A-100000 at>,
>Seng-Lai Tan <sltan at> wrote (écrivait) :
>> Dear fellow researchers,
>> i'm trying to reverse transcribe my RNA but it's resuspended in water that
>> contains some SDS, which is inhibiting the RT reaction.  Does anyone know
>> of a easy method (e.g. precipitation) that I can use to remove the SDS in
>> my sample?  many thanks in advance.
>> seng-lai

Precipitate it with NaCl as the counter-ion.  The SDS won't precipitate.
Reference: Maniatas, v. 3, pg. E11.

Josh Gamse
gamse at
Whitehead Institute, MIT


Josh Gamse            Department of Biology, MIT
gamse at      617-258-5200

"16% of Perot voters believe "if dolphins were really smart, they 
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