DNA Conc by Fluoresence spectroscopy - method required.
gotulak at sickkids.on.ca
Fri Jan 17 18:14:46 EST 1997
Gerome Breen wrote:
> I'm currently investigating accurate ways of assessing DNA (human dsDNA)
> concentration and fluoresence spec. was recommended to me - but by
> someone who didn't have a copy of a method to do it. We have a dye
> called Hoechst 33342, is it suitable? What concentrations, dilutions,
> wavelengths, etc., should I use? Any help would be much appreciated.
> Gerome Breen.
> E-Mail: g.breen at abdn.ac.uk
> University of Aberdeen.We have been using "Pico-green" reagent from Molecular Probes with
excellent sensitivity. You do have to set up a standard curve each time,
though, so it is more time consuming than the standard 260 nm absorbance
used for routine measurements.
(no affiliation, etc.etc.)
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