DNA Conc by Fluoresence spectroscopy - method required.

Gail Otulakowski gotulak at sickkids.on.ca
Fri Jan 17 18:14:46 EST 1997

Gerome Breen wrote:
> Hello,
> I'm currently investigating accurate ways of assessing DNA (human dsDNA)
> concentration and fluoresence spec. was recommended to me - but by
> someone who didn't have a copy of a method to do it. We have a dye
> called Hoechst 33342, is it suitable? What concentrations, dilutions,
> wavelengths, etc., should I use? Any help would be much appreciated.
> Regards,
>         Gerome Breen.
> E-Mail: g.breen at abdn.ac.uk
> University of Aberdeen.We have been using "Pico-green" reagent from Molecular Probes with 
excellent sensitivity.  You do have to set up a standard curve each time, 
though, so it is more time consuming than the standard 260 nm absorbance 
used for routine measurements.

Gail Otulakowski
(no affiliation, etc.etc.)

More information about the Methods mailing list