Hoy to Transfect primary human fibroblasts?

jstrassw at OPAL.TUFTS.EDU jstrassw at OPAL.TUFTS.EDU
Fri Jan 17 17:41:09 EST 1997


Hello!
I did my homework and could not find a reference with a protocol for
efficient transient transfection of primary human fibroblasts.  So, I
tried CaPO4 and the lipofection optomization kit from invitrogen; only a
few rare positived in the dish.  We have an electroporator (I posted good
protocol for COS-7 cells a few weeks back), but I'd rather hear others'
experiences before trying to establish conditions myself.

Thanks,
John

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