Chloroplast DNA isolation/mutant detection?
slf8 at PO.CWRU.EDU
Fri Jan 17 17:25:54 EST 1997
One of my former professors is looking for a protocol to use in teaching
a laboratory in plant physiology. The lab manual that he is using has
an experiment in which the students isolate chloroplast DNA and search
for mutants using RFLP analysis. One problem: the protocol calls for
an ultracentrifuge, which the college does not possess.
What I would REALLY like is a protocol for isolating chloroplast
DNA which does not require an ultracentrifuge. If not, then perhaps
could you point me to a simple lab demonstrating mutant detection
in plants? The college does have a regular centrifuge, gel apparatus,
access to enzymes, etc., but I don't think they have a thermocycler.
Your help is much appreciated.
Case Western Reserve University, Dept. of Genetics
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