[Q] How to anneal long (695b) heteroduplex DNA fragments?
Dr. J.P. Clewley
jclewley at hgmp.mrc.ac.uk
Mon Jan 20 17:09:54 EST 1997
Peter.Rudolph at MPI-Dortmund.MPG.de (Peter Rudolph) wrote:
>we want to create heteroduplex DNA by annealing the WT with a single-base
>mutant. Both fragments are double-stranded DNA cutted out of a vector and gel
The method known as HMA will do what you want, described in:
1. Delwart EL, Herring B, Rodrigo AG, Mullins JI.
Genetic subtyping of human immunodeficiency virus using a heteroduplex
PCR Methods and Applications 1995;4:S202-216.
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