[Q] How to anneal long (695b) heteroduplex DNA fragments?

[Dr. J.P. Clewley]

Peter.Rudolph at MPI-Dortmund.MPG.de (Peter Rudolph) wrote:

>we want to create heteroduplex DNA by annealing the WT with a single-base
>mutant. Both fragments are double-stranded DNA cutted out of a vector and gel
>purified.

The method known as HMA will do what you want, described in:

1.	Delwart EL, Herring B, Rodrigo AG, Mullins JI. 
Genetic subtyping of human immunodeficiency virus using a heteroduplex 
mobility assay. 
PCR Methods and Applications 1995;4:S202-216.


Jon Clewley