GC-Melt

ku at helix.nih.gov ku at helix.nih.gov
Mon Jan 20 09:37:24 EST 1997


Tetramethyl and tetraethylammonium chloride have this effect at high
concentrations. They have been used for years in hybridizations and DNA
melting experiments of various sorts where it was desirable to eliminate
the effect of differing GC-content on hybridization/melting
temperatures.


Karen Usdin
National Institutes of Health
Bethesda, MD, USA



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