Problems with Sigma enzymes
sa83207 at odin.mdacc.tmc.edu
Mon Jan 20 14:30:22 EST 1997
Gavin Willis wrote:
We have had problems with the last two enzymes purchased from Sigma: Bgl
II and PN kinase. Has anyone else had similar problems?
I have been using MluI from Sigma, and it seems to work ok. The
odd thing is that Sigma lists it as a dam-blocked enzyme, Promega
and NEB both don't. And NEB indicates that heat incativates at 65 deg.C
for 20 min, and Promega only partial inactivation. I don't know if
I'd reccommend using MluI.
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