Problems with Sigma enzymes

Jennifer Philhower sa83207 at
Mon Jan 20 14:30:22 EST 1997

Gavin Willis wrote:
We have had problems with the last two enzymes purchased from Sigma: Bgl
II and PN kinase. Has anyone else had similar problems?


I have been using MluI from Sigma, and it seems to work ok.  The
odd thing is that Sigma lists it as a dam-blocked enzyme, Promega
and NEB both don't.  And NEB indicates that heat incativates at 65 deg.C
for 20 min, and Promega only partial inactivation.  I don't know if 
I'd reccommend using MluI.

Jen Philhower

More information about the Methods mailing list