Reagent request: mouse liver, heart or other cDNA library?
cbbccm2 at tthsc6.lubb.ttuhsc.edu
Mon Jan 20 19:13:13 EST 1997
I am doing a project on polyadenylation for which I will sequence 2-300
cDNAs from both a germ cell and a somatic cell cDNA library. I have germ
cell, but I need a small amount of a library from a somatic tissue (i.e.
not testis or germ cell). Good examples would be mouse liver, heart, or
kidney. And (as if I were not asking enough already :-) ), I would like
the library to be directionally cloned into the vector using oligo(dT) as
the primer. Our current germ cell library is in Stratagene's Uni-ZAP II
vector/system, and I have had good results with that. Does someone have
such a reagent?
I am aware that Stratagene (and perhaps other vendors?) has libraries
available, but as a new assistant professor my resources are a bit
limited, so I cannot justify the $700 such a library would cost.
Many thanks for your help!
\ Dr. Clinton C. MacDonald \ cbbccm2 at tthsc6.lubb.ttuhsc.edu
\ Cell Biology & Biochemistry\ 806/743-2703
\ Texas Tech University \ 806/743-2990 (FAX)
\ Lubbock, TX 79430 \
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