RT-PCR of Total mRNA

Martin Offterdinger a8803349 at unet.univie.ac.at
Mon Jan 20 09:54:45 EST 1997


On Mon, 06 Jan 1997 11:01:46 GMT, charles.lawrie at trinity.ox.ac.uk
(charles lawrie) wrote:

>Please help . I have a limited quantity of tissue from which I will 

>prepare mRNA . My question is can I use RT-PCR to amplify the total mRNA 

>population for use in producing a labelled probe . Which primers should 

>I use would poly(A) and random hexamers be suitable for the PCR . Would 

>the amplified DNA be representitive enough to use as a probe for a cDNA 

>library . Does anybody have any recipes or know of references . Thank 

>you very much in advance .
>
>Cheers#:}

I do not think that this is an approbiate approach as random hexamers
have a low annealing temperature-so low that you will probably not get
any PCR product.
martin




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