Redissolving Mini Prep pellets?

Edgar Valencia edgar at CIFN.UNAM.MX
Tue Jan 21 17:53:06 EST 1997


Hi Wolfgang

> Has anyone yet tried to redissolve a minprep pellet (SDS/NaOH, then NaOAc).
> Will TE or Tris do? Or do I need something special?

Yes, we have used TE or just water all depends on whether we will use the
DNA for digestion, Calcium transformation, electroporation (sp?) or
whatever. We
have to avoid salts using the electroporator.
Hope this helps
Edgar




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