APS & TEMED in acid gels
sbc4 at PO.CWRU.EDU
Wed Jan 22 20:37:11 EST 1997
This does not make sense to me either. Perhaps the kinetics of the
polymerization will be increased, but it is the amount of Bis-Acrylamide and
total Acrylamide concentration that controls the quantity of cross-linking
which controls the porosity of the gel. Just like other catalysts, APS and
TEMED do not effect the final product but the speed in which equalibrium is
>>If you can the amounts of APS and TEMED, you will change the kinetics of
>>gelation (great, that's what you want).
>Agreed. You can also control kinetics by changing the temperature.
>> You will also change the porosity of
>>the gel - the relative amount of cross-linking.
>Is this really true? I was under the impression that the porosity
>was governed by the % acrylamide and by the ratio of acrylamide/bis
>(or other cross-linker). The concentration of TEMED and/or persulphate
>shouldn't have any effect unless they become limiting - so that the
>gel fails to polymerise completely no matter how long it is left.
>Since they only catalyse the polymerisation of the acrylamide monomer
>they play no real role in the eventual structure of the gel.
> Can someone correct or confirm my assumption?
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