Long PCR and Complete mt Genomes

Jim Cummins cummins at pbrc.hawaii.edu
Wed Jan 22 15:59:13 EST 1997


In article <32DCD929.67CA at umich.edu>, ajwolf at umich.edu wrote:

> There was a recent article on long PCR techniques and the amplification 
> of complete mitochondrial genomes.  
Try the following.
.  S. Melov, G.J. Lithgow, D.R. Fischer, P.M. Tedesco and  T.E. Johnson.
"Increased frequency of deletions in the mitochondrial genome with age of
Caenorhabditis elegans." Nucleic Acids Research  23. 1419 (1995).
2. B. Fromenty, G. Manfredi, J. Sadlock, L. Zhang, M.P. King and  E.A.
Schon. "Efficient and specific amplification of identified partial
duplications of human mitochondrial DNA by long PCR." Biochimica et
Biophysica Acta - Gene Structure & Expression  1308. 222 (1996).
3. F.Z. Sun, N. Arnheim and  M.S. Waterman. "Whole genome amplification of
single cells - mathematical analysis of PEP and tagged PCR." Nucleic Acids
Research  23. 3034 (1995).
4. M. Hayakawa, K. Katsumata, M. Yoneda, M. Tanaka, S. Sugiyama and  T.
Ozawa. "Mitochondrial DNA minicircles, lacking replication origins, exist
in the cardiac muscle of a young normal subject." Biochemical &
Biophysical Research Communications  215. 952 (1995).
5. M. Hayakawa, K. Katsumata, M. Yoneda, M. Tanaka, S. Sugiyama and  T.
Ozawa. "Age-related extensive fragmentation of mitochondrial DNAinto
minicircles." Biochemical & Biophysical Research Communications  226. 369
(1996).



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