APS & TEMED in acid gels

Bernard Murray bernard at elsie.nci.nih.gov
Wed Jan 22 14:51:29 EST 1997


In article <1997Jan22.123448.9875 at news.iup.edu>, jford at grove.iup.edu says...
>
>If you can the amounts of APS and TEMED, you will change the kinetics of 
>gelation (great, that's what you want).

Agreed.  You can also control kinetics by changing the temperature.

>  You will also change the porosity of 
>the gel - the relative amount of cross-linking.

Is this really true?  I was under the impression that the porosity
was governed by the % acrylamide and by the ratio of acrylamide/bis
(or other cross-linker).  The concentration of TEMED and/or persulphate
shouldn't have any effect unless they become limiting - so that the
gel fails to polymerise completely no matter how long it is left.
Since they only catalyse the polymerisation of the acrylamide monomer
they play no real role in the eventual structure of the gel.
	Can someone correct or confirm my assumption?
		Bernard

Bernard Murray, Ph.D.
bernard at elsie.nci.nih.gov (National Cancer Institute, NIH, Bethesda MD, USA)




More information about the Methods mailing list