littsj at zgi.com
Wed Jan 22 21:45:05 EST 1997
The first eqn. is a good estimate for primers, but not as good as nearest
neighbor based estimations. The second is for large fragments of average
base composition and is inappropriate for oligos as a rule.
In article <Pine.SOL.3.91.970121133519.3846E-100000 at cmu>,
ratana at CMU.CHIANGMAI.AC.TH (Ratana Banjerdpongchai) wrote:
> > >
> > > I have the problem about primers's Tm. calculation. Between 2
> > > equatios we have, one is "Tm.=4(G+C)+2(A+T)" and the other one is Tm.=
> > > 85.1+0.41(log[Na])-(675/n). my primers are not shown the same or even
> > > neary value , for example, 80.0C for the equation 1. and 61.0 for the
> > > equation 2.
> > > Can anyone suggest me about these.? the different annealing
> > > temperatures i have give me the different results . it's seem to be that
> > > the annealing temperature calculated from equatiuon 1. is better.
> > > thank you very much
> > >
> > > Pongsathorn Dhumtanom
> > > biochemistry Medicine
> > > chiangmai U.
> > > Thailand
> > >
Seattle, WA USA
My comments are my own and not my employer's.
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