DNA Conc by Fluoresence spectroscopy - method required.

Jim Litts littsj at zgi.com
Wed Jan 22 21:30:00 EST 1997


Try contacting Pharmacia which phagocytized Hoefer and now apparently sells
that technology.  They have a little application manual.

http://www.biotech.pharmacia.se/dyna/dyna.htm


 In article <32DE57EC.30A2 at abdn.ac.uk>, Gerome Breen <men106 at abdn.ac.uk> wrote:

> Hello,
> I'm currently investigating accurate ways of assessing DNA (human dsDNA)
> concentration and fluoresence spec. was recommended to me - but by
> someone who didn't have a copy of a method to do it. We have a dye
> called Hoechst 33342, is it suitable? What concentrations, dilutions,
> wavelengths, etc., should I use? Any help would be much appreciated.
> 
> Regards,
>         Gerome Breen.
> 
> E-Mail: g.breen at abdn.ac.uk
> University of Aberdeen.



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