clontech pcr-select cDNA subtraction kit

Reza Zolfaghari rxz7 at
Thu Jan 23 23:40:23 EST 1997

>I am interested in comparing two cell populations (primary culture/ 
>diseased vs normal) using the clontech pcr-select cDNA subtraction 
>kit. Has anyone used this kit? how good is it? Any comments/opinions 
>would be much appreciated.

I have used the Clontech PCR-Select kit with two different lots four 
times. All I can say is that it did not work for me. Now I am trying to 
switch to another protocol. That is PCR-Based Subtractive cDNA Cloning 
which is described in the Protocols in Molecular Biology (red books). 
This method is based on the method described originally by Wang and 

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